1887

Journal of Medical Microbiology logo

Volume 72, Issue 1

Next-generation sequencing for the diagnosis of meningoencephalitis: a case series of five consecutive patients No Access

Abstract

The prompt and specific diagnosis of meningoencephalitis (LMM) is challenging. Next-generation sequencing (NGS) of cerebrospinal fluid (CSF) is an emerging technique for diagnosing infrequent causative pathogens.

We hypothesized that NGS of CSF is an effective approach for diagnosing LMM.

To evaluate the effectiveness of NGS, we present five cases of LMM diagnosed using NGS of the CSF.

Between August 2017 and 30 September 2020, we used NGS of the CSF to detect pathogens in patients with clinically suspected central nervous system infections. The clinical characteristics, laboratory tests, imaging findings and NGS results are reviewed.

Five patients were diagnosed with LMM using NGS of the CSF within 2 to 4 days, although the clinical manifestations, medical history and imaging findings varied strikingly. NGS of CSF showed sequence reads corresponding to species ranging from 118 to 1997 bp, genomic coverage of 0.29–5.96 %, relative abundance of 14.83–32.16 % and sequencing depth of 1.12 to 1.35. The prompt diagnosis resulted in targeted and effective treatment with the appropriate antibiotics, although two patients with the most severe cerebral parenchymal lesions showed little improvement.

Our results demonstrate the power of NGS of CSF for the prompt diagnosis of LMM. NGS of CSF is an important complementary tool for identifying .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): effectiveness , Listeria monocytogenes meningoencephalitis , next-generation sequencing and prompt diagnosis
Funding
This study was supported by the:
  • Science and Technology Project for People's Livelihood (Award 20377790D)
    • Principle Award Recipient: HuiBu
© 2023 The Authors
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.001641
2023-01-09
2024-04-26
Loading full text...

Full text loading...

References

  1. Pagliano P, Ascione T, Boccia G, De Caro F, Esposito S. Listeria monocytogenes meningitis in the elderly: epidemiological, clinical and therapeutic findings. Infez Med 2016; 24:105–111
     [Google Scholar]
  2. Reuter G, Fodor D, Forgách P, Kátai A, Szucs G. Characterization and zoonotic potential of endemic hepatitis E virus (HEV) strains in humans and animals in Hungary. J Clin Virol 2009; 44:277–281 [View Article]
     [Google Scholar]
  3. Disson O, Lecuit M. Targeting of the central nervous system by Listeria monocytogenes. Virulence 2012; 3:213–221 [View Article]
     [Google Scholar]
  4. Mailles A, Lecuit M, Goulet V, Leclercq A, Stahl J-P et al. Listeria monocytogenes encephalitis in France. Med Mal Infect 2011; 41:594–601 [View Article]
     [Google Scholar]
  5. Lim S, Chung DR, Kim Y-S, Sohn KM, Kang S-J et al. Predictive risk factors for Listeria monocytogenes meningitis compared to pneumococcal meningitis: a multicenter case-control study. Infection 2017; 45:67–74 [View Article]
     [Google Scholar]
  6. Acewicz A, Witkowski G, Rola R, Ryglewicz D, Sienkiewicz-Jarosz H. An unusual presentation of Listeria monocytogenes rhombencephalitis. Neurol Neurochir Pol 2017; 51:180–183 [View Article]
     [Google Scholar]
  7. Mylonakis E, Hohmann EL, Calderwood SB. Central nervous system infection with Listeria monocytogenes. 33 years’ experience at a general hospital and review of 776 episodes from the literature. Medicine 1998; 77:313–336 [View Article]
     [Google Scholar]
  8. Paul ML, Dwyer DE, Chow C, Robson J, Chambers I et al. Listeriosis — a review of eighty‐four cases. Med JAust 1994; 160:489–493 [View Article]
     [Google Scholar]
  9. Choi MH, Park YJ, Kim M, Seo YH, Kim YA et al. Increasing incidence of Listeriosis and infection-associated clinical outcomes. Ann Lab Med 2018; 38:102–109 [View Article]
     [Google Scholar]
  10. Li N, Huang HQ, Zhang GS, Hua W, Shen HH. Encephalitis caused by Listeria monocytogenes in a healthy adult male in China: a case report. Medicine 2019; 98:e16145 [View Article]
     [Google Scholar]
  11. Brown JR, Bharucha T, Breuer J. Encephalitis diagnosis using metagenomics: application of next generation sequencing for undiagnosed cases. J Infect 2018; 76:225–240 [View Article]
     [Google Scholar]
  12. Miao Q, Ma Y, Wang Q, Pan J, Zhang Y et al. Microbiological diagnostic performance of metagenomic next-generation sequencing when applied to clinical practice. Clin Infect Dis 2018; 67:S231–S240 [View Article]
     [Google Scholar]
  13. Fan S, Ren H, Wei Y, Mao C, Ma Z et al. Next-generation sequencing of the cerebrospinal fluid in the diagnosis of neurobrucellosis. Int J Infect Dis 2018; 67:20–24 [View Article]
     [Google Scholar]
  14. Jin K WX, Guan H, Qin L, Zhou K, Jia Y et al. Application of next-generation sequencing in the brucella infection in the central nervous system. Chin J Clin Infect Dis 2020; 13:195–198
     [Google Scholar]
  15. Lan ZW, Xiao MJ, Guan YL, Zhan YJ, Tang XQ. Detection of Listeria monocytogenes in a patient with meningoencephalitis using next-generation sequencing: a case report. BMC Infect Dis 2020; 20:721 [View Article]
     [Google Scholar]
  16. Yao M, Zhou J, Zhu Y, Zhang Y, Lv X et al. Detection of Listeria monocytogenes in CSF from three patients with Meningoencephalitis by next-generation sequencing. J Clin Neurol 2016; 12:446–451 [View Article]
     [Google Scholar]
  17. Deneke C, Rentzsch R, Renard BY. PaPrBaG: a machine learning approach for the detection of novel pathogens from NGS data. Sci Rep 2017; 7:39194 [View Article]
     [Google Scholar]
  18. Li M LY, Hu B, Guo L, Guo X, Guan H et al. Clinical characteristics and next generation sequencing of three cases of Listeria monocytogenes meningitis with complications. Chin J Pediatr 2019; 57:
     [Google Scholar]
  19. Merle-Melet M, Dossou-Gbete L, Maurer P, Meyer P, Lozniewski A et al. Is amoxicillin-cotrimoxazole the most appropriate antibiotic regimen for listeria meningoencephalitis? Review of 22 cases and the literature. J Infect 1996; 33:79–85 [View Article]
     [Google Scholar]
  20. Mylonakis E, Paliou M, Hohmann EL, Calderwood SB, Wing EJ. Listeriosis during pregnancy: a case series and review of 222 cases. Medicine 2002; 81:260–269 [View Article]
     [Google Scholar]
  21. Carryn S, Van Bambeke F, Mingeot-Leclercq MP, Tulkens PM. Comparative intracellular (THP-1 macrophage) and extracellular activities of beta-lactams, azithromycin, gentamicin, and fluoroquinolones against Listeria monocytogenes at clinically relevant concentrations. Antimicrob Agents Chemother 2002; 46:2095–2103 [View Article]
     [Google Scholar]
  22. Mongkolrattanothai K, Naccache SN, Bender JM, Samayoa E, Pham E et al. Neurobrucellosis: unexpected answer from metagenomic next-generation sequencing. J Pediatric Infect Dis Soc 2017; 6:393–398 [View Article]
     [Google Scholar]
  23. Pokorski M. Respiratory Treatment and Prevention. In Sensitivity of Next-Generation Sequencing Metagenomic Analysis for Detection of RNA and DNA Viruses in Cerebrospinal Fluid: The Confounding Effect of Background Contamination Cham: Respiratory Treatment and Prevention; 2017 [View Article]
     [Google Scholar]
  24. Wilson MR, Naccache SN, Samayoa E, Biagtan M, Bashir H et al. Actionable diagnosis of neuroleptospirosis by next-generation sequencing. N Engl J Med 2014; 370:2408–2417 [View Article]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.001641
Loading
Next-generation sequencing for the diagnosis of Listeria monocytogenes meningoencephalitis: a case series of five consecutive patients
J. Med. Microbiol. 72, 001641 (2023); https://doi.org/10.1099/jmm.0.001641
/content/journal/jmm/10.1099/jmm.0.001641
/content/journal/jmm/10.1099/jmm.0.001641
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error