1887

Abstract

Sepsis is one of the leading causes of death worldwide. The rapid identification (ID) of the causative micro-organisms is crucial for the patients’ clinical outcome. MALDI-TOF MS has been widely investigated to speed up the time-to-report for ID from positive blood cultures, and many different procedures and protocols were developed, all of them attributable either to the direct separation of microbial cells from the blood cells, or to a short subculture approach. In this study, the Rapid Sepsityper workflow (MBT Sepsityper IVD Kit, Bruker Daltonics GmbH and Co. KG, Bremen, Germany) was compared to three different short subculturing methods, established into the routine practice of three different clinical microbiology laboratories. A total of =503 routine samples were included in this study and tested in parallel with the two approaches. Results of the rapid procedures were finally compared to routine proceedings with Gram-staining and overnight subculture. Among monomicrobial samples, the Rapid Sepsityper workflow enabled overall the correct identification of 388/443 (87.6 %) micro-organisms, while the short subculturing methods of 267/435 (61.8 %). Except for the performance with , in each one of the three sites the Rapid Sepsityper workflow proved to be superior to the short subculture method, regardless of the protocol applied, and it delivered a result from 1 to 5 h earlier.

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2022-08-05
2022-08-14
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References

  1. Adhikari NKJ, Fowler RA, Bhagwanjee S, Rubenfeld GD. Critical care and the global burden of critical illness in adults. Lancet 2010; 376:1339–1346 [View Article] [PubMed]
    [Google Scholar]
  2. Kumar A, Roberts D, Wood KE, Light B, Parrillo JE et al. Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med 2006; 34:1589–1596 [View Article] [PubMed]
    [Google Scholar]
  3. Fraser A, Paul M, Almanasreh N, Tacconelli E, Frank U et al. Benefit of appropriate empirical antibiotic treatment: thirty-day mortality and duration of hospital stay. Am J Med 2006; 119:970–976 [View Article] [PubMed]
    [Google Scholar]
  4. Paul M, Shani V, Muchtar E, Kariv G, Robenshtok E et al. Systematic review and meta-analysis of the efficacy of appropriate empiric antibiotic therapy for sepsis. Antimicrob Agents Chemother 2010; 54:4851–4863 [View Article] [PubMed]
    [Google Scholar]
  5. Pogue JM, Kaye KS, Cohen DA, Marchaim D. Appropriate antimicrobial therapy in the era of multidrug-resistant human pathogens. Clin Microbiol Infect 2015; 21:302–312 [View Article] [PubMed]
    [Google Scholar]
  6. Rhodes A, Evans LE, Alhazzani W, Levy MM, Antonelli M et al. Surviving sepsis campaign: International guidelines for management of sepsis and septic shock: 2016. Intensive Care Med 2017; 43:304–377 [View Article] [PubMed]
    [Google Scholar]
  7. Cattoir L, Coorevits L, Leroux-Roels I, Claeys G, Verhasselt B et al. Improving timelines in reporting results from positive blood cultures: simulation of impact of rapid identification on therapy on a real-life cohort. Eur J Clin Microbiol Infect Dis 2018; 37:2253–2260 [View Article] [PubMed]
    [Google Scholar]
  8. Lagacé-Wiens PRS, Adam HJ, Karlowsky JA, Nichol KA, Pang PF et al. Identification of blood culture isolates directly from positive blood cultures by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and a commercial extraction system: analysis of performance, cost, and turnaround time. J Clin Microbiol 2012; 50:3324–3328 [View Article] [PubMed]
    [Google Scholar]
  9. Faron ML, Buchan BW, Ledeboer NA. Matrix-assisted laser desorption Ionization-time of flight mass spectrometry for use with positive blood cultures: methodology, performance, and optimization. J Clin Microbiol 2017; 55:3328–3338 [View Article] [PubMed]
    [Google Scholar]
  10. Idelevich EA, Schüle I, Grünastel B, Wüllenweber J, Peters G et al. Rapid identification of microorganisms from positive blood cultures by MALDI-TOF mass spectrometry subsequent to very short-term incubation on solid medium. Clin Microbiol Infect 2014; 20:1001–1006 [View Article] [PubMed]
    [Google Scholar]
  11. Bhatti MM, Boonlayangoor S, Beavis KG, Tesic V. Rapid identification of positive blood cultures by matrix-assisted laser desorption ionization-time of flight mass spectrometry using prewarmed agar plates. J Clin Microbiol 2014; 52:4334–4338 [View Article] [PubMed]
    [Google Scholar]
  12. Kohlmann R, Hoffmann A, Geis G, Gatermann S. MALDI-TOF mass spectrometry following short incubation on a solid medium is a valuable tool for rapid pathogen identification from positive blood cultures. Int J Med Microbiol 2015; 305:469–479 [View Article] [PubMed]
    [Google Scholar]
  13. Verroken A, Defourny L, Lechgar L, Magnette A, Delmée M et al. Reducing time to identification of positive blood cultures with MALDI-TOF MS analysis after a 5-h subculture. Eur J Clin Microbiol Infect Dis 2015; 34:405–413 [View Article] [PubMed]
    [Google Scholar]
  14. Chen Y, Porter V, Mubareka S, Kotowich L, Simor AE. Rapid identification of bacteria directly from positive blood cultures by use of a serum separator tube, smudge plate preparation, and matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2015; 53:3349–3352 [View Article] [PubMed]
    [Google Scholar]
  15. Curtoni A, Cipriani R, Marra ES, Barbui AM, Cavallo R et al. Rapid identification of microorganisms from positive blood culture by maldi-tof ms after short-term incubation on solid medium. Curr Microbiol 2017; 74:97–102 [View Article] [PubMed]
    [Google Scholar]
  16. Altun O, Botero-Kleiven S, Carlsson S, Ullberg M, Özenci V. Rapid identification of bacteria from positive blood culture bottles by MALDI-TOF MS following short-term incubation on solid media. J Med Microbiol 2015; 64:1346–1352 [View Article] [PubMed]
    [Google Scholar]
  17. Torres I, Gimenez E, Pascual T, Bueno F, Huntley D et al. Short-term incubation of positive blood cultures in brain-heart infusion broth accelerates identification of bacteria by matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry. J Med Microbiol 2017; 66:1752–1758 [View Article] [PubMed]
    [Google Scholar]
  18. Idelevich EA, Seifert H, Sundqvist M, Scudeller L, Amit S et al. ESCMID study group for bloodstream infections endocarditis and sepsis (ESGBIES) microbiological diagnostics of bloodstream infections in europe-an ESGBIES survey. Clin Microbiol Infect 2019; Nov;25(11:1399–1407 [View Article]
    [Google Scholar]
  19. Yonezawa T, Watari T, Ashizawa K, Hanada D, Yanagiya T et al. Development of an improved rapid BACpro® protocol and a method for direct identification from blood-culture-positive bottles using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. J Microbiol Methods 2018; 148:138–144 [View Article] [PubMed]
    [Google Scholar]
  20. Schubert S, Weinert K, Wagner C, Gunzl B, Wieser A et al. Novel, improved sample preparation for rapid, direct identification from positive blood cultures using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. J Mol Diagn 2011; 13:701–706 [View Article] [PubMed]
    [Google Scholar]
  21. Morgenthaler NG, Kostrzewa M. Rapid identification of pathogens in positive blood culture of patients with sepsis: review and meta-analysis of the performance of the sepsityper kit. Int J Microbiol 2015; 2015:827416 [View Article] [PubMed]
    [Google Scholar]
  22. Egli A, Osthoff M, Goldenberger D, Halter J, Schaub S et al. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) directly from positive blood culture flasks allows rapid identification of bloodstream infections in immunosuppressed hosts. Transpl Infect Dis 2015; 17:481–487 [View Article] [PubMed]
    [Google Scholar]
  23. Scohy A, Noël A, Boeras A, Brassinne L, Laurent T et al. Evaluation of the Bruker® MBT Sepsityper IVD module for the identification of polymicrobial blood cultures with MALDI-TOF MS. Eur J Clin Microbiol Infect Dis 2018; 37:2145–2152 [View Article] [PubMed]
    [Google Scholar]
  24. Cordovana M, Zignoli A, Ambretti S. Rapid Sepsityper in clinical routine: 2 years’ successful experience. J Med Microbiol 2020; 69:1398–1404 [View Article] [PubMed]
    [Google Scholar]
  25. Ponderand L, Pavese P, Maubon D, Giraudon E, Girard T et al. Evaluation of rapid sepsityper® protocol and specific MBT-sepsityper module (bruker daltonics) for the rapid diagnosis of bacteremia and fungemia by MALDI-TOF-MS. Ann Clin Microbiol Antimicrob 2020; 19:60 [View Article] [PubMed]
    [Google Scholar]
  26. Saffert RT, Cunningham SA, Mandrekar J, Patel R. Comparison of three preparatory methods for detection of bacteremia by MALDI-TOF mass spectrometry. Diagn Microbiol Infect Dis 2012; 73:21–26 [View Article] [PubMed]
    [Google Scholar]
  27. Klein S, Zimmermann S, Köhler C, Mischnik A, Alle W et al. Integration of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in blood culture diagnostics: a fast and effective approach. J Med Microbiol 2012; 61:323–331 [View Article] [PubMed]
    [Google Scholar]
  28. Jamal W, Saleem R, Rotimi VO. Rapid identification of pathogens directly from blood culture bottles by bruker matrix-assisted laser desorption laser ionization-time of flight mass spectrometry versus routine methods. Diagn Microbiol Infect Dis 2013; 76:404–408 [View Article] [PubMed]
    [Google Scholar]
  29. Torres I, Gimenez E, Pascual T, Bueno F, Huntley D et al. Short-term incubation of positive blood cultures in brain-heart infusion broth accelerates identification of bacteria by matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry. J Med Microbiol 2017; 66:1752–1758 [View Article] [PubMed]
    [Google Scholar]
  30. Martín-Pujol O, Tosco-Nuñez T, de Miguel-Martinez I. Comparison of three procedures for the rapid identification of bacteraemia-causing microorganisms. evaluation of their effectiveness and applicability to microbiology laboratories. Enferm Infecc Microbiol Clin (Engl Ed) 2019; 37:319–323 [View Article] [PubMed]
    [Google Scholar]
  31. Fang C, Zhou Z, Li J, Chen X, Zhou M. Rapid identification of microorganisms from positive blood cultures in pediatric patients by MALDI-TOF MS: sepsityper kit versus short-term subculture. J Microbiol Methods 2020; 172:105894 [View Article] [PubMed]
    [Google Scholar]
  32. Ibrahim EH, Sherman G, Ward S, Fraser VJ, Kollef MH. The influence of inadequate antimicrobial treatment of bloodstream infections on patient outcomes in the ICU setting. Chest 2000; 118:146–155 [View Article] [PubMed]
    [Google Scholar]
  33. Buehler SS, Madison B, Snyder SR, Derzon JH, Cornish NE et al. effectiveness of practices to increase timeliness of providing targeted therapy for inpatients with bloodstream infections: a laboratory medicine best practices systematic review and meta-analysis. Clin Microbiol Rev 2016; 29:59–103 [View Article] [PubMed]
    [Google Scholar]
  34. Idelevich EA, Grunewald CM, Wüllenweber J, Becker K. Rapid identification and susceptibility testing of Candida spp. from positive blood cultures by combination of direct MALDI-TOF mass spectrometry and direct inoculation of Vitek 2. PLoS One 2014; 9:e114834 [View Article]
    [Google Scholar]
  35. Hazelton B, Thomas LC, Olma T, Kok J, O’Sullivan M et al. Rapid and accurate direct antibiotic susceptibility testing of blood culture broths using MALDI sepsityper combined with the BD Phoenix automated system. J Med Microbiol 2014; 63:1590–1594 [View Article] [PubMed]
    [Google Scholar]
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