1887

Journal of Medical Microbiology logo

Volume 71, Issue 5

JMM profile: Loop-mediated isothermal amplification (LAMP): for the rapid detection of nucleic acid targets in resource-limited settings Open Access

This article is part of the JMM Profiles collection.

Abstract

Loop-mediated isothermal amplification (LAMP) is a rapid alternative to PCR, in which the reaction occurs at one temperature and uses a polymerase with high displacement activity, e.g. DNA polymerase I () or homologues. Since the discovery of LAMP in 2000, several applications have been developed to employ this technique in the rapid detection of nucleic acid targets and enhance its performance. Improvements to the LAMP technique and a variety of innovative detection methods have led to its application for a wide range of targets in medical and veterinary microbiology, particularly in resource-poor settings. The key advantages of LAMP-based diagnostics include the ability to rapidly detect target nucleic acid sequences within 30 min and its ease of use, facilitating its application in field, bedside, pen-side, point-of-care and point-of-need diagnostic settings. LAMP can be a valuable tool to aid in the detection and management of disease outbreaks.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): AMR , diagnostics , LAMP and nucleic acid detection
Funding
This study was supported by the:
  • Innovate UK
    • Principle Award Recipient: AuroreC Poirier
  • Innovate UK
    • Principle Award Recipient: RobertoM La Ragione
  • Biotechnology and Biological Sciences Research Council
    • Principle Award Recipient: RobertoM La Ragione
  • Biotechnology and Biological Sciences Research Council
    • Principle Award Recipient: AuroreC Poirier
  • Liphook Equine Hospital/Vet Partners Ltd
    • Principle Award Recipient: LucyF Grist
  • One Health European Joint Programme (OHEJP); WorldCOM project (Award European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 773830)
    • Principle Award Recipient: MarwaM Hassan
  • One Health European Joint Programme (OHEJP); WorldCOM project (Award European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 773830)
    • Principle Award Recipient: RobertoM La Ragione
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.001522
2022-05-19
2024-05-13
Loading full text...

Full text loading...

/deliver/fulltext/jmm/71/5/jmm001522.html?itemId=/content/journal/jmm/10.1099/jmm.0.001522&mimeType=html&fmt=ahah

References

  1. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 2000; 28:12 [View Article] [PubMed]
     [Google Scholar]
  2. Mori Y, Nagamine K, Tomita N, Notomi T. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun 2001; 289:150–154 [View Article] [PubMed]
     [Google Scholar]
  3. Mori Y, Notomi T. Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases. J Infect Chemother 2009; 15:62–69 [View Article] [PubMed]
     [Google Scholar]
  4. Diribe O, North S, Sawyer J, Roberts L, Fitzpatrick N et al. Design and application of a loop-mediated isothermal amplification assay for the rapid detection of Staphylococcus pseudintermedius. J Vet Diagn Invest 2014; 26:42–48 [View Article] [PubMed]
     [Google Scholar]
  5. Rohaim MA, Clayton E, Sahin I, Vilela J, Khalifa ME et al. Artificial intelligence-assisted loop mediated isothermal amplification (AI-LAMP) for rapid detection of SARS-CoV-2. Viruses 2020; 12:E972 [View Article] [PubMed]
     [Google Scholar]
  6. Chen H-T, Zhang J, Sun D-H, Ma L-N, Liu X-T et al. Development of reverse transcription loop-mediated isothermal amplification for rapid detection of H9 avian influenza virus. J Virol Methods 2008; 151:200–203 [View Article] [PubMed]
     [Google Scholar]
  7. Higgins O, Smith TJ. Loop-primer endonuclease cleavage-loop-mediated isothermal amplification technology for multiplex pathogen detection and single-nucleotide polymorphism identification. J Mol Diagn 2020; 22:640–651 [View Article] [PubMed]
     [Google Scholar]
  8. Wang Y, Wang Y, Lan R, Xu H, Ma A et al. Multiple endonuclease restriction real-time loop-mediated isothermal amplification: a novel analytically rapid, sensitive, multiplex loop-mediated isothermal amplification detection technique. J Mol Diagn 2015; 17:392–401 [View Article] [PubMed]
     [Google Scholar]
  9. Wang Y, Wang Y, Luo L, Liu D, Luo X et al. Rapid and sensitive detection of Shigella spp. and Salmonella spp. by multiple endonuclease restriction real-time loop-mediated isothermal amplification technique. Front Microbiol 2015; 6:1400 [View Article] [PubMed]
     [Google Scholar]
  10. Liu N, Zou D, Dong D, Yang Z, Ao D et al. Development of a multiplex loop-mediated isothermal amplification method for the simultaneous detection of Salmonella spp. and Vibrio parahaemolyticus. Sci Rep 2017; 7:45601 [View Article] [PubMed]
     [Google Scholar]
  11. Zhang H, Xu Y, Fohlerova Z, Chang H, Iliescu C et al. LAMP-on-a-chip: Revising microfluidic platforms for loop-mediated DNA amplification. Trends Analyt Chem 2019; 113:44–53 [View Article] [PubMed]
     [Google Scholar]
  12. Nguyen HQ, Bui HK, Phan VM, Seo TS. An internet of things-based point-of-care device for direct reverse-transcription-loop mediated isothermal amplification to identify SARS-CoV-2. Biosens Bioelectron 2022; 195:113655 [View Article] [PubMed]
     [Google Scholar]
  13. Wang J, Staheli JP, Wu A, Kreutz JE, Hu Q et al. Detection of 14 high-risk human papillomaviruses using digital LAMP assays on a self-digitization chip. Anal Chem 2021; 93:3266–3272 [View Article] [PubMed]
     [Google Scholar]
  14. Cao X, Zhao L, Zhang J, Chen X, Shi L et al. Detection of viable but nonculturable Vibrio parahaemolyticus in shrimp samples using improved real-time PCR and real-time LAMP methods. Food Control 2019; 103:145–152 [View Article]
     [Google Scholar]
  15. Becherer L, Borst N, Bakheit M, Frischmann S, Zengerle R et al. Loop-mediated isothermal amplification (LAMP) – review and classification of methods for sequence-specific detection. Anal Methods 2020; 12:717–746 [View Article]
     [Google Scholar]
  16. Wang X, Seo DJ, Lee MH, Choi C. Comparison of conventional PCR, multiplex PCR, and loop-mediated isothermal amplification assays for rapid detection of Arcobacter species. J Clin Microbiol 2014; 52:557–563 [View Article] [PubMed]
     [Google Scholar]
  17. Kaneko H, Kawana T, Fukushima E, Suzutani T. Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances. J Biochem Biophys Methods 2007; 70:499–501 [View Article] [PubMed]
     [Google Scholar]
  18. Ikadai H, Tanaka H, Shibahara N, Matsuu A, Uechi M et al. Molecular evidence of infections with Babesia gibsoni parasites in Japan and evaluation of the diagnostic potential of a loop-mediated isothermal amplification method. J Clin Microbiol 2004; 42:2465–2469 [View Article] [PubMed]
     [Google Scholar]
  19. Kuboki N, Inoue N, Sakurai T, Di Cello F, Grab DJ et al. Loop-mediated isothermal amplification for detection of African trypanosomes. J Clin Microbiol 2003; 41:5517–5524 [View Article] [PubMed]
     [Google Scholar]
  20. Parida M, Horioke K, Ishida H, Dash PK, Saxena P et al. Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay. J Clin Microbiol 2005; 43:2895–2903 [View Article] [PubMed]
     [Google Scholar]
  21. Seki M, Yamashita Y, Torigoe H, Tsuda H, Sato S et al. Loop-mediated isothermal amplification method targeting the lytA gene for detection of Streptococcus pneumoniae. J Clin Microbiol 2005; 43:1581–1586 [View Article] [PubMed]
     [Google Scholar]
  22. Chander Y, Koelbl J, Puckett J, Moser MJ, Klingele AJ et al. A novel thermostable polymerase for RNA and DNA loop-mediated isothermal amplification (LAMP). Front Microbiol 2014; 5:395 [View Article] [PubMed]
     [Google Scholar]
  23. Kashir J, Yaqinuddin A. Loop mediated isothermal amplification (LAMP) assays as a rapid diagnostic for COVID-19. Med Hypotheses 2020; 141:109786 [View Article] [PubMed]
     [Google Scholar]
  24. Tharmakulasingam M, Chaudhry NS, Branavan M, Balachandran W, Poirier AC et al. An artificial intelligence-assisted portable low-cost device for the rapid detection of SARS-CoV-2. Electronics 2021; 10:2065 [View Article]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.001522
Loading
JMM profile: Loop-mediated isothermal amplification (LAMP): for the rapid detection of nucleic acid targets in resource-limited settings
J. Med. Microbiol. 71, 001522 (2022); https://doi.org/10.1099/jmm.0.001522
/content/journal/jmm/10.1099/jmm.0.001522
/content/journal/jmm/10.1099/jmm.0.001522
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error