1887

Abstract

Acquired resistance against the antibiotics that are active against species has been described.

Diagnostics combined with antimicrobial sensitivity testing are required for therapeutic guidance.

To report the prevalence of antimicrobial resistance among Cuban isolates and the related molecular mechanisms of resistance.

Traditional broth microdilution assays were used for antimicrobial sensitivity testing in 262 clinical species isolates from Cuban patients between 2013 and 2018, and a subset of samples were investigated in parallel with the commercial MYCO WELL D-ONE rapid culture diagnostic assay. The underlying molecular mechanisms for resistance were determined by PCR and sequencing for all resistant isolates.

Among the tested isolates, the tetracycline and erythromycin resistance rates were 1.9 and 1.5%, respectively, while fluoroquinolone resistance was not found. The (M) gene was found in all tetracycline-resistant isolates, but also in two tetracycline-susceptible linical isolates. We were unable to determine the underlying mechanism of erythromycin resistance. The MYCO WELL D-ONE kit overestimated tetracycline and erythromycin resistance in spp. isolates.

Although low levels of antibiotic resistance were detected in Cuban patients over a 5-year period, continued surveillance of the antibiotic susceptibility of is necessary to monitor possible changes in resistance patterns.

Funding
This study was supported by the:
  • Cardiff University
    • Principle Award Recipient: nadiaRodríguez rodriguez
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/content/journal/jmm/10.1099/jmm.0.001477
2022-02-17
2022-07-06
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