1887

Abstract

Outbreaks of carbapenem-resistant and have occurred worldwide in healthcare settings. Rapid and reliable molecular typing of bacterial isolates is vital for the effective surveillance of institutional outbreaks. The Pan-PCR and OXA-PCR assays are two multiplex PCR-based assays for the molecular typing of species.

However, few studies have investigated the discriminatory power of two multiplex PCR assays in in the genotyping of species.

We aimed to evaluate the efficacies of the Pan-PCR and OXA-PCR assays for molecular typing of and .

A total of 105 carbapenem-resistant isolates (CRABs) and 93 carbapenem-resistant isolates (CRANs) obtained from blood cultures were used for molecular typing by the Pan-PCR and OXA-PCR assays and two multilocus sequence typing (MLST) schemes.

The isolates were individually divided into 12 and 21 different sequence types via the Pasteur and Oxford MLST schemes, respectively. Additionally, these isolates were distinguished into 18 different types by the Pan-PCR and OXA-PCR assays. The results of the Pan-PCR and OXA-PCR assays distinguished CRABs and CRANs with a sensitivity of 98.13 % and a specificity of 100 %.

The Pan-PCR and OXA-PCR assays are promising alternative methods for rapid molecular typing of CRABs and CRANs in a routine laboratory setting.

Funding
This study was supported by the:
  • Tri-Service General Hospital (Award TSGH-D-109049)
    • Principle Award Recipient: Chuan-ChungChuang
  • Tri-Service General Hospital (Award TSGH-C106-024)
    • Principle Award Recipient: Chuan-ChungChuang
  • Ministry of Science and Technology (Award MOST 108-2320-B-016-009)
    • Principle Award Recipient: JunRen Sun
  • Taipei Hospital (Award TPCH-110-14)
    • Principle Award Recipient: Hsing-YuChen
  • Taipei Hospital (Award TPCH-109-20)
    • Principle Award Recipient: Hsing-YuChen
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2021-07-08
2021-07-27
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