1887

Abstract

The increasing incidence of with decreased susceptibility to quinolones (quinolone low-susceptible ) in Japan has raised concerns about therapeutic failure. Thus, assessment of effective antimicrobial agents is necessary to establish an effective therapeutic strategy against resulting infections. In this study, bactericidal effects of quinolones on low-susceptible strains were evaluated using time-kill curve analysis. For tosufloxacin, log reduction values for low-susceptible strains were significantly lower than those for susceptible strains at both C and 1/2 C. Conversely, although the log reduction values were lower for susceptible strains, the C of levofloxacin and β-lactams (amoxicillin and cefditoren) indicated bactericidal effects. In addition, higher concentrations of tosufloxacin at 2×C and 4×C had bactericidal effects on not only susceptible but also low-susceptible strains. These data strongly suggest that we should consider the presence of low-susceptible strains and reconsider the appropriate dosage of tosufloxacin for treatment, especially for paediatric patients.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.001376
2021-06-24
2021-07-29
Loading full text...

Full text loading...

References

  1. Kakuta R, Yano H, Hidaka H, Kanamori H, Endo S et al. Molecular epidemiology of ampicillin-resistant Haemophilus influenzae causing acute otitis media in Japanese infants and young children. Pediatr Infect Dis J 2016; 35:501–506 [View Article] [PubMed]
    [Google Scholar]
  2. Park C, Kim KH, Shin NY, Byun JH, Kwon EY et al. Genetic diversity of the ftsI gene in beta-lactamase-nonproducing ampicillin-resistant and beta-lactamase-producing amoxicillin-/clavulanic acid-resistant nasopharyngeal Haemophilus influenzae strains isolated from children in south korea. Microb Drug Resist 2013; 19:224–230 [View Article] [PubMed]
    [Google Scholar]
  3. Ouchi K, Sunakawa K. Effect of new oral antimicrobial agents in outpatient treatment of pneumonia in children. Jpn J Antibiotics 2014; 67:157–166
    [Google Scholar]
  4. Morozumi M, Chiba N, Okada T, Sakata H, Matsubara K et al. Antibiotic susceptibility in relation to genotype of Streptococcus pneumoniae, Haemophilus influenzae, and Mycoplasma pneumoniae responsible for community-acquired pneumonia in children. J Infect Chemother 2013; 19:432–440 [View Article] [PubMed]
    [Google Scholar]
  5. Tanaka T, Oishi T, Miyata I, Wakabayashi S, Kono M et al. Macrolide-resistant Mycoplasma pneumoniae infection, Japan, 2008-2015. Emerg Infect Dis 2017; 23:1703–1706 [View Article] [PubMed]
    [Google Scholar]
  6. Shoji H, Shirakura T, Fukuchi K, Takuma T, Hanaki H et al. A molecular analysis of quinolone-resistant Haemophilus influenzae: Validation of the mutations in quinolone resistance-determining regions. J Infect Chemother 2014; 20:250–255 [View Article] [PubMed]
    [Google Scholar]
  7. Seyama S, Wajima T, Yanagisawa Y, Nakaminami H, Ushio M et al. Rise in Haemophilus influenzae with reduced quinolone susceptibility and development of a simple screening method. Pediatr Infect Dis J 2017; 36:263–266 [View Article] [PubMed]
    [Google Scholar]
  8. Tanaka E, Hara N, Wajima T, Ochiai S, Seyama S et al. Emergence of Haemophilus influenzae with low susceptibility to quinolones and persistence in tosufloxacin treatment. J Glob Antimicrob Resist 2019; 18:104–108 [View Article] [PubMed]
    [Google Scholar]
  9. CLSI Performance Standards for Antimicrobial Susceptibility Testing, 29th ed. Wayne, PA: CLSI; 2019
    [Google Scholar]
  10. Hara N, Wajima T, Tanaka E, Noguchi N, Shirai A et al. Two cases in which tosufloxacin was administered for respiratory infections that may have been caused by Haemophilus influenzae less susceptible to quinolone. Jpn J Pharm Health Care Sci 2020; 46:211–214
    [Google Scholar]
  11. Tanaka E, Wajima T, Hara N, Shirai A, Shibata M et al. First outbreak of Haemophilus influenzae clone ST422 with low susceptibility to quinolones in paediatric patients in japan. J Med Microbiol 2020; 69:239–243 [View Article] [PubMed]
    [Google Scholar]
  12. Kanda Y. Investigation of the freely available easy-to-use software “EZR” for medical statistics. Bone Marrow Transplant 2013; 48:452–458 [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.001376
Loading
/content/journal/jmm/10.1099/jmm.0.001376
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error