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Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 70, Issue 6

Evaluation of the rapid ResaPolymyxin / NP test for rapid colistin resistance detection in lactose non-fermenting Gram-negative bacteria No Access

Abstract

Colistin is one of the last-resort antibiotics for treating multidrug-resistant (MDR) or extensively drug-resistant (XDR) lactose non-fermenting Gram-negative bacteria such as and .

As the rate of colistin resistance is steadily rising, there is a need for rapid and accurate antimicrobial susceptibility testing methods for colistin. The Rapid ResaPolymyxin / NP test has recently been developed for rapid detection of colistin resistance in and .

The present study aimed to evaluate the performance of the Rapid ResaPolymyxin / NP test in comparison with the reference broth microdilution (BMD) method.

The Rapid ResaPolymyxin / NP test was performed using a total of 135 . (17 colistin-resistant and 118 colistin-susceptible) and 66 isolates (32 colistin-resistant and 34 colistin-susceptible), in comparison with the reference BMD method.

The categorical agreement of the Rapid ResaPolymyxin / NP test with the reference BMD method was 97.5 % with a major error rate of 0 % (0/152) and a very major error (VME) rate of 10.2 %. The VME rate was higher (23.5 %) when calculated separately for isolates. The overall sensitivity and specificity were 89.8 and 100 %, respectively.

The Rapid ResaPolymyxin / NP test performed better for than for .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Acinetobacter baumannii , colistin , Pseudomonas aeruginosa and rapid resistance detection test
© 2021 The Authors
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2021-06-24
2025-02-14
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Evaluation of the rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test for rapid colistin resistance detection in lactose non-fermenting Gram-negative bacteria
J. Med. Microbiol. 70, 001373 (2021); https://doi.org/10.1099/jmm.0.001373
/content/journal/jmm/10.1099/jmm.0.001373
/content/journal/jmm/10.1099/jmm.0.001373
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