1887

Journal of Medical Microbiology logo

Volume 70, Issue 6

Characterization of a carbapenem-resistant coharbouring and genes No Access

Abstract

Members of the genus are facultative anaerobic Gram-negative bacilli belonging to the [Janda 1994; 32(8):1850–1854; Arens 1997;3(1):53–57]. Formerly, were occasionally reported as nosocomial pathogens with low virulence [Pepperell 2002;46(11):3555–60]. Now, they are consistently reported to cause nosocomial infections of the urinary tract, respiratory tract, bone, peritoneum, endocardium, meninges, intestines, bloodstream and central nervous system. Among species, the most common isolates are and , while has seldom been isolated [Janda 1994; 32(8):1850–1854; Marak 2017;49(7):532–9]. Further, spp. are usually susceptible to carbapenems, aminoglycosides, tetracyclines and colistin [Marak 2017;49(7):532–9].

As is rare, only one clinical isolate, coharbouring carbapenem resistance gene and quinolone resistance gene , has been reported.

To characterize a carbapenem-resistant strain from PR China coharbouring and .

Three hundred and forty nonrepetitive carbapenem-resistant (CRE) strains were collected during 2011–2018. A carbapenem-resistant strain was detected and confirmed using a VITEK mass spectrometry-based microbial identification system and 16S rRNA sequencing. Minimum inhibitory concentrations (MICs) for clinical antimicrobials were obtained by the broth microdilution method. Whole-genome sequencing (WGS) was performed for antibiotic resistance gene analysis, and a phylogenetic tree of strains was constructed using the Bacterial Pan Genome Analysis (BPGA) tool. The transferability of the resistance plasmid was verified by conjugal transfer.

A rare carbapenem-resistant strain (CA71) was recovered from a patient with cerebral obstruction and the sequences of 16S rRNA gene shared more than 99 % similarity with CITRO86, FDAARGOS 165. CA71 is resistant to β-lactam, quinolone and aminoglycoside antibiotics, and even imipenem and meropenem (MICs of 2 and 4 mg l respectively), and is only sensitive to polymyxin B and tigecycline. Six antibiotic resistance genes were detected via WGS, including the β-lactam genes , and , the quinolone gene , and the aminoglycoside genes . Interestingly, and coexist on an IncN1-type plasmid (pCA71-IMP) and successfully transferred to J53 via conjugal transfer. Phylogenetic analysis showed that CA71 is most similar to strain CJ25 and belongs to the same evolutionary cluster along with seven other strains.

To the best of our knowledge, this is the first report of a carbapenem-resistant isolate coharbouring and .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): carbapenem resistance , Citrobacter amalonaticus and whole-genome sequencing analysis
Funding
This study was supported by the:
  • Technology Planning Project for Medicine and Healthcare of Zhejiang Province (Award 2021KY1237)
    • Principle Award Recipient: ZhigangZhao
  • the Major Research and Development Project of Lishui City of China (Award 2020ZDYF11)
    • Principle Award Recipient: ZhigangZhao
  • the Major Research and Development Project of Lishui City of China (Award 2017ZDYF13)
    • Principle Award Recipient: JianshengHuang
  • National Natural Science Foundation of China (Award 81802044)
    • Principle Award Recipient: JianshengHuang
© 2021 The Authors
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.001364
2021-06-25
2024-05-13
Loading full text...

Full text loading...

References

  1. Wayne P. Performance Standards for Antimicrobial Susceptibility Testing [S] Twenty-seventh Informational Supplement Clinical and Laboratory Standards InstituteM100‐S127;
     [Google Scholar]
  2. Chaudhari NM, Gupta VK, Dutta C. BPGA- an ultra-fast pan-genome analysis pipeline. Sci Rep 2016; 6:24373 [View Article] [PubMed]
     [Google Scholar]
  3. Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004; 32:1792–1797 [View Article] [PubMed]
     [Google Scholar]
  4. Janda JM, Abbott SL, Cheung WK, Hanson DF. Biochemical identification of Citrobacteria in the clinical laboratory. J Clin Microbiol 1994; 32:1850–1854 [View Article] [PubMed]
     [Google Scholar]
  5. Lipsky BA, Hook EW, Smith AA, Plorde JJ. Citrobacter infections in humans: Experience at the Seattle Veterans Administration Medical Center and a review of the literature. Clin Infect Dis 1980; 2:746–760 [View Article]
     [Google Scholar]
  6. Lai CC, Tan CK, Lin SH, Liu WL, Liao CH et al. Bacteraemia caused by non-freundii, non-koseri Citrobacter species in Taiwan. J Hosp Infect 2010; 76:332–335 [View Article] [PubMed]
     [Google Scholar]
  7. Aschbacher R, Pagani L, Doumith M, Pike R, Woodford N et al. Metallo-β-lactamases among Enterobacteriaceae from routine samples in an Italian tertiary-care hospital and long-term care facilities during 2008. Clin Microbiol Infect 2011; 17:181–189 [View Article] [PubMed]
     [Google Scholar]
  8. Maraki S, Vardakas KZ, Mavromanolaki V-E, Kyriakidou M, Spais G et al. In vitro susceptibility and resistance phenotypes in contemporary Citrobacter isolates in a university hospital in Crete, Greece. Infect Dis (Lond) 2017; 49:532–539 [View Article] [PubMed]
     [Google Scholar]
  9. Arana DM, Ortega A, González-Barberá E, Lara N, Bautista V et al. Carbapenem-resistant Citrobacter spp. isolated in Spain from 2013 to 2015 produced a variety of carbapenemases including VIM-1, OXA-48, KPC-2, NDM-1 and VIM-2. J Antimicrob Chemother 2017; 72:3283–3287 [View Article]
     [Google Scholar]
  10. Faccone D, Albornoz E, Tijet N, Biondi E, Gomez S et al. Characterization of a multidrug-resistant Citrobacter amalonaticus clinical isolate harboring blaNDM-1 and mcr-1.5genes. Infect Genet Evol 2019; 67:51–54 [View Article] [PubMed]
     [Google Scholar]
  11. Chu YW, Afzal-Shah M, Houang ET, Palepou MI, Lyon DJ et al. IMP-4, a novel metallo-beta-lactamase from nosocomial Acinetobacter spp. collected in Hong Kong between 1994 and 1998. Antimicrob Agents Chemother 2001; 45:710–714 [View Article] [PubMed]
     [Google Scholar]
  12. Peleg AY, Franklin C, Bell J, Spelman DW. Emergence of IMP-4 metallo-beta-lactamase in a clinical isolate from Australia. J Antimicrob Chemother 2004; 54:699–700 [View Article] [PubMed]
     [Google Scholar]
  13. Kizny Gordon A, Phan HTT, Lipworth SI, Cheong E, Gottlieb T et al. Genomic dynamics of species and mobile genetic elements in a prolonged blaIMP-4-associated carbapenemase outbreak in an Australian hospital. J Antimicrob Chemother 2020; 75:873–882 [View Article] [PubMed]
     [Google Scholar]
  14. Limbago BM, Rasheed JK, Anderson KF, Zhu W, Kitchel B et al. IMP-producing carbapenem-resistant Klebsiella pneumoniae in the United States. J Clin Microbiol 2011; 49:4239–4245 [View Article] [PubMed]
     [Google Scholar]
  15. Bert F, Vanjak D, Leflon-Guibout V, Mrejen S, Delpierre S et al. IMP-4-producing Pseudomonas aeruginosa in a French patient repatriated from Malaysia: impact of early detection and control measures. Clin Infect Dis 2007; 44:764–765 [View Article] [PubMed]
     [Google Scholar]
  16. Koh TH, Sng LH, Wang GC, Hsu LY, Zhao Y. IMP-4 and OXA beta-lactamases in Acinetobacter baumannii from Singapore. J Antimicrob Chemother 2007; 59:627–632 [View Article] [PubMed]
     [Google Scholar]
  17. Khosravi Y, Tay ST, Vadivelu J. Analysis of integrons and associated gene cassettes of metallo-β-lactamase-positive Pseudomonas aeruginosa in Malaysia. J Med Microbiol 2011; 60:988–994 [View Article] [PubMed]
     [Google Scholar]
  18. Dolejska M, Masarikova M, Dobiasova H, Jamborova I, Karpiskova R et al. High prevalence of Salmonella and IMP-4-producing Enterobacteriaceae in the silver gull on Five Islands, Australia. J Antimicrob Chemother 2016; 71:63–70 [View Article] [PubMed]
     [Google Scholar]
  19. Liang Q, Jiang X, Hu L, Yin Z, Gao B et al. Sequencing and genomic diversity analysis of IncHI5 plasmids. Front Microbiol 2018; 9:3318 [View Article] [PubMed]
     [Google Scholar]
  20. PL H, WU L, Chan J, Cheung YY, Chow KH et al. pIMP-PH114 carrying blaIMP-4 in a Klebsiella pneumoniae strain is closely related to other multidrug-resistant IncA/C2 plasmids. Curr Microbiol 2014; 68:227–232
     [Google Scholar]
  21. Wang Y, W-U L, RW-M L, CW-S T, Lee RA et al. INCN ST7 epidemic plasmid carrying blaimp-4 in Enterobacteriaceae isolates with epidemiological links to multiple geographical areas in China. J Antimicrobial Chem 2017; 72:99–103
     [Google Scholar]
  22. Tamang MD, Seol SY, Oh J-Y, Kang HY, Lee JC et al. Plasmid-mediated quinolone resistance determinants qnrA, qnrB, and qnrS among clinical isolates of Enterobacteriaceae in a Korean hospital. Antimicrob Agents Chemother 2008; 52:4159–4162 [View Article]
     [Google Scholar]
  23. Dolejska M, Villa L, Hasman H, Hansen L, Alessandra C. Characterization of INCN plasmids carrying BLACTX-M-1 and QNR genes in Escherichia coli and salmonella from animals, the environment, and humans. J Antimicrob Chemother 2013; 68:333–339 [View Article] [PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.001364
Loading
Characterization of a carbapenem-resistant Citrobacter amalonaticus coharbouring blaIMP-4 and qnrs1 genes
J. Med. Microbiol. 70, 001364 (2021); https://doi.org/10.1099/jmm.0.001364
/content/journal/jmm/10.1099/jmm.0.001364
/content/journal/jmm/10.1099/jmm.0.001364
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error