Emergence of carbapenem-resistant Klebsiella pneumoniae harbouring blaOXA-48-like genes in China

Ying Chen; Li Fang; Yunxing Yang; Rushuang Yan; Ying Fu; Ping Shen; Dongdong Zhao; Yan Chen; Xiaoting Hua; Yan Jiang; Robert A. Moran; Willem van Schaik; Yunsong Yu

doi:10.1099/jmm.0.001306

Volume 70, Issue 3

Short Communication

Open Access

Emergence of carbapenem-resistant Klebsiella pneumoniae harbouring bla OXA-48-like genes in China

Ying Chen^1,2,3,†, Li Fang^1,2,3,†, Yunxing Yang^4,†, Rushuang Yan^1,2,3, Ying Fu⁵, Ping Shen⁶, Dongdong Zhao^1,2,3, Yan Chen^1,2,3, Xiaoting Hua^1,2,3, Yan Jiang^1,2,3, Robert A. Moran⁷, Willem van Schaik⁷ and Yunsong Yu^1,2,3
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Affiliations: ¹ Department of Infectious Disease, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, PR China ² Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, PR China ³ Regional Medical Center for National Institute of Respiratory Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, PR China ⁴ Department of Clinical Laboratory, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou, PR China ⁵ Department of Clinical Laboratory, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, PR China ⁶ State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, PR China ⁷ Institute of Microbiology and Infection, University of Birmingham, Birmingham, UK
*Correspondence: Willem van Schaik, [email protected] *Correspondence: Yunsong Yu, [email protected]

† These authors contributed equally to this work
Published: 28 January 2021 https://doi.org/10.1099/jmm.0.001306

Abstract

Klebsiella pneumoniae strains carrying OXA-48-like carbapenemases are increasingly prevalent across the globe. There is thus an urgent need to better understand the mechanisms that underpin the dissemination of bla OXA-48-like carbapenemases. To this end, four ertapenem-resistant K. pneumoniae isolates producing OXA-48-like carbapenemases were isolated from two patients. Genome sequencing revealed that one sequence type (ST) 17 isolate carried bla OXA-181, whilst three isolates from a single patient, two ST76 and one ST15, carried bla OXA-232. The 50514 bp bla OXA-181-harbouring plasmid, pOXA-181_YML0508, was X3-type with a conjugation frequency to Escherichia coli of 1.94×10−4 transconjugants per donor. The bla OXA-232 gene was located on a 6141 bp ColKP3-type plasmid, pOXA-232_WSD, that was identical in the ST76 and ST15 K. pneumoniae isolates. This plasmid could be transferred from K. pneumoniae to E. coli at low frequency, 8.13×10−6 transconjugants per donor. Comparative analysis revealed that the X3 plasmid acquired the bla OXA-48-like gene via IS3000-mediated co-integration of the ColKP3-type plasmid. Our study highlights how plasmid integration and rearrangements can contribute to the spread of bla OXA-48-like genes, which provides important clues for clinical prevention of the dissemination of K. pneumoniae strains carrying bla OXA-48-like carbapenemases.

Received: 18/08/2020
Accepted: 15/12/2020
Published Online: 28/01/2021

Keyword(s): carbapenem-resistant Klebsiella pneumoniae , China , Klebsiella pneumoniae , OXA-181 and OXA-232

Funding

This study was supported by the:

the Medical Research Council (Award MR/S013660/1)
- Principle Award Recipient: Willemvan Schaik
the National Science and Technology Major Project (Award 2018ZX10714002)
- Principle Award Recipient: PingShen
the Key Research and Development Programme of Zhejiang (Award 2015C03046)
- Principle Award Recipient: YunsongYu
the National Natural Science Foundation of China (Award 81802043)
- Principle Award Recipient: DongdongZhao
the National Natural Science Foundation of China (Award 81830069)
- Principle Award Recipient: YunsongYu

This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.

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Emergence of carbapenem-resistant Klebsiella pneumoniae harbouring blaOXA-48-like genes in China

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Volume 70, Issue 3

Short Communication

Open Access

Emergence of carbapenem-resistant Klebsiella pneumoniae harbouring bla OXA-48-like genes in China

Abstract

Funding

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