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Abstract

strains carrying OXA-48-like carbapenemases are increasingly prevalent across the globe. There is thus an urgent need to better understand the mechanisms that underpin the dissemination of -like carbapenemases. To this end, four ertapenem-resistant isolates producing OXA-48-like carbapenemases were isolated from two patients. Genome sequencing revealed that one sequence type (ST) 17 isolate carried , whilst three isolates from a single patient, two ST76 and one ST15, carried . The 50514 bp -harbouring plasmid, pOXA-181_YML0508, was X3-type with a conjugation frequency to of 1.94×10 transconjugants per donor. The gene was located on a 6141 bp ColKP3-type plasmid, pOXA-232_WSD, that was identical in the ST76 and ST15 isolates. This plasmid could be transferred from to at low frequency, 8.13×10 transconjugants per donor. Comparative analysis revealed that the X3 plasmid acquired the -like gene via IS-mediated co-integration of the ColKP3-type plasmid. Our study highlights how plasmid integration and rearrangements can contribute to the spread of -like genes, which provides important clues for clinical prevention of the dissemination of strains carrying -like carbapenemases.

Funding
This study was supported by the:
  • Willemvan Schaik , the Medical Research Council , (Award MR/S013660/1)
  • PingShen , the National Science and Technology Major Project , (Award 2018ZX10714002)
  • YunsongYu , the Key Research and Development Programme of Zhejiang , (Award 2015C03046)
  • DongdongZhao , the National Natural Science Foundation of China , (Award 81802043)
  • YunsongYu , the National Natural Science Foundation of China , (Award 81830069)
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/content/journal/jmm/10.1099/jmm.0.001306
2021-01-28
2021-03-02
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