1887

Abstract

and , belonging to the phylogenetic group, are occasionally isolated from clinical samples, partly because they are often misidentified as in clinical laboratories. There are five reports describing carbapenem-resistant clinical isolates of these species. Carbapenem-resistant strains of and were isolated from stool samples. These isolates were sequenced using Illumina MiSeq and reidentified using average nucleotide identity (ANI) based on comparisons of their whole-genome sequences using the OrthoANI algorithm. The clonal relatedness of the isolates was assessed by pulse-field gel electrophoresis (PFGE). The size of plasmids conveying was examined by Southern blotting. A total of six carbapenem-resistant clinical isolates of (two isolates) and (four isolates) were obtained from stool samples from five patients in a Japanese hospital. All isolates harboured . The two isolates of had a different pattern in the PFGE analysis, with both having a 23 kb plasmid. Of the four isolates of with similar patterns in the PFGE analysis, three had 320 kb plasmids and one had a 240 kb plasmid. The genetic environments of the 320/240 kb and 23 kb plasmids differed. The results strongly indicated that carbapenem-resistant and producing metallo-β-lactamase are emerging in Japan. This is the first report of carbapenem-resistant and in Japan.

Funding
This study was supported by the:
  • JU Research Fund
    • Principle Award Recipient: Teruo Kirikae
  • Japan Agency for Medical Research and Development (Award 20fk0108061h0303)
    • Principle Award Recipient: Teruo Kirikae
  • Japan Society for the Promotion of Science (Award 20K16252)
    • Principle Award Recipient: Tomomi Hishinuma
  • Japan Society for the Promotion of Science (Award 19KK0203)
    • Principle Award Recipient: Teruo Kirikae
  • Japan Society for the Promotion of Science (Award 19K16652)
    • Principle Award Recipient: Mari Tohya
  • Japan Society for the Promotion of Science (Award 18K07121)
    • Principle Award Recipient: Teruo Kirikae
  • Japan Society for the Promotion of Science (Award 18K07120)
    • Principle Award Recipient: Tatsuya Tada
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2020-11-23
2021-10-16
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