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Abstract
Pseudomonas asiatica and Pseudomonas monteilii , belonging to the Pseudomonas putida phylogenetic group, are occasionally isolated from clinical samples, partly because they are often misidentified as P. putida in clinical laboratories. There are five reports describing carbapenem-resistant clinical isolates of these species. Carbapenem-resistant strains of P. asiatica and P. monteilii were isolated from stool samples. These isolates were sequenced using Illumina MiSeq and reidentified using average nucleotide identity (ANI) based on comparisons of their whole-genome sequences using the OrthoANI algorithm. The clonal relatedness of the isolates was assessed by pulse-field gel electrophoresis (PFGE). The size of plasmids conveying bla VIM-2 was examined by Southern blotting. A total of six carbapenem-resistant clinical isolates of P. asiatica (two isolates) and P. monteilii (four isolates) were obtained from stool samples from five patients in a Japanese hospital. All isolates harboured blaVIM-2 . The two isolates of P. asiatica had a different pattern in the PFGE analysis, with both having a 23 kb plasmid. Of the four isolates of P. monteilii with similar patterns in the PFGE analysis, three had 320 kb plasmids and one had a 240 kb plasmid. The genetic environments of the 320/240 kb and 23 kb plasmids differed. The results strongly indicated that carbapenem-resistant P. asiatica and P. monteilii producing metallo-β-lactamase are emerging in Japan. This is the first report of carbapenem-resistant P. asiatica and P. monteilii in Japan.
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Funding
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JU Research Fund
- Principle Award Recipient: Teruo Kirikae
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Japan Agency for Medical Research and Development
(Award 20fk0108061h0303)
- Principle Award Recipient: Teruo Kirikae
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Japan Society for the Promotion of Science
(Award 20K16252)
- Principle Award Recipient: Tomomi Hishinuma
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Japan Society for the Promotion of Science
(Award 19KK0203)
- Principle Award Recipient: Teruo Kirikae
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Japan Society for the Promotion of Science
(Award 19K16652)
- Principle Award Recipient: Mari Tohya
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Japan Society for the Promotion of Science
(Award 18K07121)
- Principle Award Recipient: Teruo Kirikae
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Japan Society for the Promotion of Science
(Award 18K07120)
- Principle Award Recipient: Tatsuya Tada