1887

Journal of Medical Microbiology logo

Volume 70, Issue 1

Emergence of clinical isolates of and from Japan harbouring an acquired gene encoding a carbapenemase VIM-2 Free

Abstract

and , belonging to the phylogenetic group, are occasionally isolated from clinical samples, partly because they are often misidentified as in clinical laboratories. There are five reports describing carbapenem-resistant clinical isolates of these species. Carbapenem-resistant strains of and were isolated from stool samples. These isolates were sequenced using Illumina MiSeq and reidentified using average nucleotide identity (ANI) based on comparisons of their whole-genome sequences using the OrthoANI algorithm. The clonal relatedness of the isolates was assessed by pulse-field gel electrophoresis (PFGE). The size of plasmids conveying was examined by Southern blotting. A total of six carbapenem-resistant clinical isolates of (two isolates) and (four isolates) were obtained from stool samples from five patients in a Japanese hospital. All isolates harboured . The two isolates of had a different pattern in the PFGE analysis, with both having a 23 kb plasmid. Of the four isolates of with similar patterns in the PFGE analysis, three had 320 kb plasmids and one had a 240 kb plasmid. The genetic environments of the 320/240 kb and 23 kb plasmids differed. The results strongly indicated that carbapenem-resistant and producing metallo-β-lactamase are emerging in Japan. This is the first report of carbapenem-resistant and in Japan.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): carbapenem resistance , metallo-β-lactamase , Pseudomonas asiatica , Pseudomonas monteilii and VIM-2
Funding
This study was supported by the:
  • JU Research Fund
    • Principle Award Recipient: Teruo Kirikae
  • Japan Agency for Medical Research and Development (Award 20fk0108061h0303)
    • Principle Award Recipient: Teruo Kirikae
  • Japan Society for the Promotion of Science (Award 20K16252)
    • Principle Award Recipient: Tomomi Hishinuma
  • Japan Society for the Promotion of Science (Award 19KK0203)
    • Principle Award Recipient: Teruo Kirikae
  • Japan Society for the Promotion of Science (Award 19K16652)
    • Principle Award Recipient: Mari Tohya
  • Japan Society for the Promotion of Science (Award 18K07121)
    • Principle Award Recipient: Teruo Kirikae
  • Japan Society for the Promotion of Science (Award 18K07120)
    • Principle Award Recipient: Tatsuya Tada
© 2021 The Authors
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.001258
2020-11-23
2024-05-04
Loading full text...

Full text loading...

/deliver/fulltext/jmm/70/1/jmm001258.html?itemId=/content/journal/jmm/10.1099/jmm.0.001258&mimeType=html&fmt=ahah

References

  1. Gniadek TJ, Carroll KC, Simner PJ. Carbapenem-resistant non-glucose-fermenting Gram-negative bacilli: the missing piece to the puzzle. J Clin Microbiol 2016; 54:1700–1710 [View Article][PubMed]
     [Google Scholar]
  2. Tohya M, Watanabe S, Teramoto K, Uechi K, Tada T et al. Pseudomonas asiatica sp. nov., isolated from hospitalized patients in Japan and Myanmar. Int J Syst Evol Microbiol 2019; 69:1361–1368 [View Article][PubMed]
     [Google Scholar]
  3. Keshavarz-Tohid V, Vacheron J, Dubost A, Prigent-Combaret C, Taheri P et al. Genomic, phylogenetic and catabolic re-assessment of the Pseudomonas putida clade supports the delineation of Pseudomonas alloputida sp. nov., Pseudomonas inefficax sp. nov., Pseudomonas persica sp. nov., and Pseudomonas shirazica sp. nov. Syst Appl Microbiol 2019; 42:468–480 [View Article][PubMed]
     [Google Scholar]
  4. Tohya M, Watanabe S, Tada T, Tin HH, Kirikae T. Genome analysis-based reclassification of Pseudomonas fuscovaginae and Pseudomonas shirazica as later heterotypic synonyms of Pseudomonas asplenii and Pseudomonas asiatica, respectively. Int J Syst Evol Microbiol 2020; 70:3547–3552 [View Article][PubMed]
     [Google Scholar]
  5. Tohya M, Tada T, Watanabe S, Kuwahara-Arai K, Zin KN et al. Emergence of carbapenem-resistant Pseudomonas asiatica producing NDM-1 and VIM-2 metallo-β-lactamases in Myanmar. Antimicrob Agents Chemother 2019; 63:e00475–19 [View Article][PubMed]
     [Google Scholar]
  6. Elomari M, Coroler L, Verhille S, Izard D, Leclerc H. Pseudomonas monteilii sp. nov., isolated from clinical specimens. Int J Syst Bacteriol 1997; 47:846–852 [View Article][PubMed]
     [Google Scholar]
  7. Barka A, Jansens H, Elst E, Wojciechowski M. A Pseudomonas monteilii unilateral inguinofemoral granulomatous lymphadenitis in a two-year-old girl. Acta Clin Belg 2020; 17:1–3 [View Article]
     [Google Scholar]
  8. Taboada M, Barbeito-Castiñeiras G, Almeida X, Caruezo V, Cereijo J et al. Unusual infective prosthetic valve endocarditis due to Pseudomonas monteilii and Acinetobacter nosocomialis with a fatal result. Rev Esp Anestesiol Reanim 2019; 66:487–489 [View Article][PubMed]
     [Google Scholar]
  9. Ocampo-Sosa AA, Guzmán-Gómez L, Fernández-Martínez M, Román E, Rodríguez C et al. Isolation of VIM-2-producing Pseudomonas monteilii clinical strains disseminated in a tertiary hospital in northern Spain. Antimicrob Agents Chemother 2015; 59:1334–1336 [View Article][PubMed]
     [Google Scholar]
  10. Shariff M, Beri K, Aditi SM. Exacerbation of bronchiectasis by Pseudomonas monteilii: a case report. BMC Infect Dis 2017; 17:51 [View Article]
     [Google Scholar]
  11. Scotta C, Juan C, Cabot G, Oliver A, Lalucat J et al. Environmental microbiota represents a natural reservoir for dissemination of clinically relevant metallo-beta-lactamases. Antimicrob Agents Chemother 2011; 55:5376–5379 [View Article][PubMed]
     [Google Scholar]
  12. Bogaerts P, Bouchahrouf W, Lissoir B, Denis O, Glupczynski Y. IMP-13-producing Pseudomonas monteilii recovered in a hospital environment. J Antimicrob Chemother 2011; 66:2434–2435 [View Article][PubMed]
     [Google Scholar]
  13. National Committee for Clinical Laboratory Standards Performance Standards for Antimicrobial Susceptibility Testing; 28rd Informational Supplement, M100-S28 Wayne, PA: Clinical and Laboratory Standards Institute; 2018
     [Google Scholar]
  14. Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017; 110:1281–1286 [View Article][PubMed]
     [Google Scholar]
  15. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461–466 [View Article][PubMed]
     [Google Scholar]
  16. Bonnin RA, Poirel L, Nordmann P, Eikmeyer FG, Wibberg D et al. Complete sequence of broad-host-range plasmid pNOR-2000 harbouring the metallo-β-lactamase gene blaVIM-2 from Pseudomonas aeruginosa. J Antimicrob Chemother 2013; 68:1060–1065 [View Article][PubMed]
     [Google Scholar]
  17. Tada T, Miyoshi-Akiyama T, Shimada K, Shiroma A, Nakano K et al. A carbapenem-resistant Pseudomonas aeruginosa isolate harboring two copies of blaIMP-34 encoding a metallo-β-lactamase. PLoS One 2016; 11:e0149385 [View Article][PubMed]
     [Google Scholar]
  18. Botelho J, Roberts AP, León-Sampedro R, Grosso F, Peixe L. Carbapenemases on the move: it’s good to be on ICEs. Mob DNA 2018; 9:37 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.001258
Loading
Emergence of clinical isolates of Pseudomonas asiatica and Pseudomonas monteilii from Japan harbouring an acquired gene encoding a carbapenemase VIM-2
J. Med. Microbiol. 70, 001258 (2021); https://doi.org/10.1099/jmm.0.001258
/content/journal/jmm/10.1099/jmm.0.001258
/content/journal/jmm/10.1099/jmm.0.001258
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error