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Abstract

Multidrug-resistant tuberculosis (MDR-TB) is a major public health problem globally, including in Indonesia. Whole-genome sequencing (WGS) analysis has rarely been used for the study of TB and MDR-TB in Indonesia.

We evaluated the use of WGS for drug-susceptibility testing (DST) and to investigate the population structure of drug-resistant in Java, Indonesia.

Thirty suspected MDR-TB isolates were subjected to MGIT 960 system (MGIT)-based DST and to WGS. Phylogenetic analysis was done using the WGS data. Results obtained using MGIT-based DST and WGS-based DST were compared.

Agreement between WGS and MGIT was 93.33 % for rifampicin, 83.33 % for isoniazid and 76.67 % for streptomycin but only 63.33 % for ethambutol. Moderate WGS–MGIT agreement was found for second-line drugs including amikacin, kanamycin and fluoroquinolone (73.33–76.67 %). MDR-TB was more common in isolates of the East Asian Lineage (63.3%). No evidence of clonal transmission of DR-TB was found among members of the tested population.

Our study demonstrated the applicability of WGS for DST and molecular epidemiology of DR-TB in Java, Indonesia. We found no transmission of DR-TB in Indonesia.

Funding
This study was supported by the:
  • Kiatichai Faksri , National Research Council of Thailand , (Award Grant No. 60-057 and 62-003)
  • Angkana Chaiprasert , Fogarty International Center , (Award No. 5D43TW009522)
  • Pratiwi Sudarmono , Universitas Indonesia , (Award PITTA 2018)
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2020-06-24
2020-08-11
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