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Volume 69, Issue 2

A mouse model of small intestinal infection Open Access

Abstract

is a recognised cause of foodborne intoxication and antibiotic-associated diarrhoea (AAD), which are both mediated by staphylococcal enterotoxins. However, unlike foodborne intoxication, AAD appears to require infection of the host. While intoxication is widely studied, little is known about pathogenesis in the context of gastrointestinal infection.

To develop a mouse model of gastrointestinal infection.

An established AAD mouse model was adapted for infection, and damage observed via histopathological analysis and immunostaining of intestinal tissues.

Various strains colonised the mouse model, and analysis showed that although clinical signs of disease were not seen, infection induced damage in the small intestine, disrupting host structures essential for epithelial integrity. Studies using a staphylococcal enterotoxin B mutant showed that this toxin may contribute to damage during gastrointestinal infection.

This work presents a new mouse model of gastrointestinal infection, while also providing insight into the pathogenesis of in the gut.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antibiotic-associated diarrhoea , bacterial pathogenesis , gastrointestinal infection , infection model , mouse model and Staphylococcus aureus
Funding
This study was supported by the:
  • Australian Government Research Training Program Scholarship (Award PhD Scholarship)
    • Principle Award Recipient: Sarah Larcombe
  • Australian Research Council (Award FT120100779)
    • Principle Award Recipient: Dena Lyras
© 2020 Not applicable
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2020-01-31
2024-05-06
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A mouse model of Staphylococcus aureus small intestinal infection
J. Med. Microbiol. 69, 290 (2020); https://doi.org/10.1099/jmm.0.001163
/content/journal/jmm/10.1099/jmm.0.001163
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