1887

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Volume 69, Issue 2

Presence of genes and characterization of s, i and m regions in isolated from Alaskans and their association with clinical pathologies Free

Abstract

Gastric cancer is a health disparity in the Alaska Native people. The incidence of infection, a risk factor for non-cardia gastric adenocarcinoma, is also high. Gastric cancer is partially associated with the virulence of the infecting strain.

To genotype the s, m and i and pathogenicity island () genes in from Alaskans and investigate associations with gastropathy.

We enrolled patients with gastritis, peptic ulcer disease (PUD) and intestinal metaplasia (IM) in 1998–2005 and patients with gastric cancer in 2011–2013. Gastric biopsies were collected and cultured and PCR was performed to detect the presence of the right and left ends of the , the , , and genes and to genotype the s, m and i regions.

We recruited 263 people; 22 (8 %) had no/mild gastritis, 121 (46 %) had moderate gastritis, 40 (15%) had severe gastritis, 38 (14 %) had PUD, 30 (11 %) had IM and 12 (5 %) had gastric cancer. isolates from 150 (57%) people had an intact ; those were associated with a more severe gastropathy (≤0.02 for all comparisons). isolates from 77 % of people had either the s1/i1/m1 (40 %; 94/234) or s2/i2/m2 (37 %; 86/234) genotype. s1/i1/m1 was associated with a more severe gastropathy (≤0.03 for all comparisons).

In this population with high rates of gastric cancer, we found that just over half of the contained an intact and 40 % had the s1/i1/m1 genotype. Infection with these strains was associated with a more severe gastropathy.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Alaska , cagPAI , gastric cancer , Helicobacter pylori and vacA
Funding
This study was supported by the:
  • Alaska Science and Technology Foundation (Award n/a)
    • Principle Award Recipient: Not Applicable
  • Indian Health Service (Award U269400005)
    • Principle Award Recipient: Not Applicable
  • Indian Health Service (Award U26IHS300410)
    • Principle Award Recipient: Not Applicable
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2020-02-03
2024-04-27
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Presence of cagPAI genes and characterization of vacA s, i and m regions in Helicobacter pylori isolated from Alaskans and their association with clinical pathologies
J. Med. Microbiol. 69, 218 (2020); https://doi.org/10.1099/jmm.0.001123
/content/journal/jmm/10.1099/jmm.0.001123
/content/journal/jmm/10.1099/jmm.0.001123
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