1887

Abstract

Patient-derived isolates of the opportunistic pathogen are frequently resistant to antibiotics due to the presence of sequence variants in resistance-associated genes. However, the frequency of antibiotic resistance and of resistance-associated sequence variants in environmental isolates of has not been well studied. Antimicrobial susceptibility testing (ciprofloxacin, ceftazidime, meropenem, tobramycin) of environmental (=50) and cystic fibrosis (=42) isolates was carried out. Following whole genome sequencing of all isolates, 25 resistance-associated genes were analysed for the presence of likely function-altering sequence variants. Environmental isolates were susceptible to all antibiotics with one exception, whereas patient-derived isolates had significant frequencies of resistance to each antibiotic and a greater number of likely resistance-associated genetic variants. These findings indicate that the natural environment does not act as a reservoir of antibiotic-resistant supporting a model in which antibiotic susceptible environmental bacteria infect patients and develop resistance during infection.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.001085
2019-11-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/68/11/1591.html?itemId=/content/journal/jmm/10.1099/jmm.0.001085&mimeType=html&fmt=ahah

References

  1. Gellatly SL, Hancock REW. Pseudomonas aeruginosa: new insights into pathogenesis and host defenses. Pathog Dis 2013; 67:159–173 [View Article]
    [Google Scholar]
  2. Elborn JS. Cystic fibrosis. Lancet 2016; 388:2519–2531 [View Article]
    [Google Scholar]
  3. Lister PD, Wolter DJ, Hanson ND. Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clin Microbiol Rev 2009; 22:582–610 [View Article]
    [Google Scholar]
  4. Decraene V, Ghebrehewet S, Dardamissis E, Huyton R, Mortimer K et al. An outbreak of multidrug-resistant Pseudomonas aeruginosa in a burns service in the North of England: challenges of infection prevention and control in a complex setting. J Hosp Infect 2018; 100:e239–e245 [View Article]
    [Google Scholar]
  5. Kidd TJ, Ritchie SR, Ramsay KA, Grimwood K, Bell SC et al. Pseudomonas aeruginosa exhibits frequent recombination, but only a limited association between genotype and ecological setting. PLoS One 2012; 7:e44199 [View Article]
    [Google Scholar]
  6. Purdy-Gibson ME, France M, Hundley TC, Eid N, Remold SK. Pseudomonas aeruginosa in CF and non-CF homes is found predominantly in drains. J Cyst Fibros 2015; 14:341–346 [View Article]
    [Google Scholar]
  7. Bhuiya M, Sarkar MKI, Sohag MH, Ali H, Roy CK et al. Enumerating Antibiotic Susceptibility Patterns of Pseudomonas aeruginosa Isolated from Different Sources in Dhaka City. Open Microbiol J 2018; 12:172–180 [View Article]
    [Google Scholar]
  8. Devarajan N, Köhler T, Sivalingam P, van Delden C, Mulaji CK et al. Antibiotic resistant Pseudomonas spp. in the aquatic environment: a prevalence study under tropical and temperate climate conditions. Water Res 2017; 115:256–265 [View Article]
    [Google Scholar]
  9. Ebrahimpour M, Nikokar I, Ghasemi Y, Sedigh Ebrahim-Saraie H, Araghian A et al. Antibiotic resistance and frequency of class 1 integrons among Pseudomonas aeruginosa isolates obtained from wastewaters of a burn center in Northern Iran. Ann Ig 2018; 30:112–119 [View Article]
    [Google Scholar]
  10. Kümmerer K. Antibiotics in the aquatic environment-a review-part II. Chemosphere 2009; 75:435–441 [View Article]
    [Google Scholar]
  11. Odumosu BT, Ajetunmobi O, Dada-Adegbola H, Odutayo I. Antibiotic susceptibility pattern and analysis of plasmid profiles of Pseudomonas aeruginosa from human, animal and plant sources. Springerplus 2016; 5:1381 [View Article]
    [Google Scholar]
  12. Olga P, Apostolos V, Alexis G, George V, Athena M. Antibiotic resistance profiles of Pseudomonas aeruginosa isolated from various Greek aquatic environments. FEMS Microbiol Ecol 2016; 92:fiw042 [View Article]
    [Google Scholar]
  13. Ruiz Lı́dia, Domı́nguez MA, Ruiz N, Viñas M. Relationship between clinical and environmental isolates of Pseudomonas aeruginosa in a hospital setting. Arch Med Res 2004; 35:251–257 [View Article]
    [Google Scholar]
  14. Spindler A, Otton LM, Fuentefria DB, Corção G. Beta-Lactams resistance and presence of class 1 integron in Pseudomonas spp. isolated from untreated Hospital effluents in Brazil. Antonie Van Leeuwenhoek 2012; 102:73–81 [View Article]
    [Google Scholar]
  15. Tuméo E, Gbaguidi-Haore H, Patry I, Bertrand X, Thouverez M et al. Are antibiotic-resistant Pseudomonas aeruginosa isolated from hospitalised patients recovered in the hospital effluents?. Int J Hyg Environ Health 2008; 211:200–204 [View Article]
    [Google Scholar]
  16. Walsh TR, Weeks J, Livermore DM, Toleman MA. Dissemination of NDM-1 positive bacteria in the new Delhi environment and its implications for human health: an environmental point prevalence study. Lancet Infect Dis 2011; 11:355–362 [View Article]
    [Google Scholar]
  17. Schiavano GF, Carloni E, Andreoni F, Magi S, Chironna M et al. Prevalence and antibiotic resistance of Pseudomonas aeruginosa in water samples in central Italy and molecular characterization of oprD in imipenem resistant isolates. PLoS One 2017; 12:e0189172 [View Article]
    [Google Scholar]
  18. Gad GF, El-Domany RA, Zaki S, Ashour HM. Characterization of Pseudomonas aeruginosa isolated from clinical and environmental samples in Minia, Egypt: prevalence, antibiogram and resistance mechanisms. J Antimicrob Chemother 2007; 60:1010–1017 [View Article]
    [Google Scholar]
  19. Kidd TJ, Ramsay KA, Vidmar S, Carlin JB, Bell SC et al. Pseudomonas aeruginosa genotypes acquired by children with cystic fibrosis by age 5-years. J Cyst Fibros 2015; 14:361–369 [View Article]
    [Google Scholar]
  20. Ranganathan SC, Skoric B, Ramsay KA, Carzino R, Gibson A-M et al. Geographical differences in first acquisition of Pseudomonas aeruginosa in cystic fibrosis. Ann Am Thorac Soc 2013; 10:108–114 [View Article]
    [Google Scholar]
  21. Rosenfeld M, Emerson J, McNamara S, Thompson V, Ramsey BW et al. Risk factors for age at initial Pseudomonas acquisition in the cystic fibrosis epic observational cohort. J Cyst Fibros 2012; 11:446–453 [View Article]
    [Google Scholar]
  22. Römling U, Wingender J, Müller H, Tümmler B. A major Pseudomonas aeruginosa clone common to patients and aquatic habitats. Appl Environ Microbiol 1994; 60:1734–1738
    [Google Scholar]
  23. Kittinger C, Lipp M, Baumert R, Folli B, Koraimann G et al. Antibiotic resistance patterns of Pseudomonas spp. isolated from the river Danube. Front Microbiol 2016; 7:586 [View Article]
    [Google Scholar]
  24. Liew SM, Rajasekaram G, Puthucheary SA, Chua KH. Antimicrobial susceptibility and virulence genes of clinical and environmental isolates of Pseudomonas aeruginosa . PeerJ 2019; 7:e6217 [View Article]
    [Google Scholar]
  25. Anuj SN, Whiley DM, Kidd TJ, Bell SC, Wainwright CE et al. Identification of Pseudomonas aeruginosa by a duplex real-time polymerase chain reaction assay targeting the ecfX and the gyrB genes. Diagn Microbiol Infect Dis 2009; 63:127–131 [View Article]
    [Google Scholar]
  26. Jolley KA, Bray JE, Maiden MCJ. Open-Access bacterial population genomics: BIGSdb software, the PubMLST.org website and their applications. Wellcome Open Res 2018; 3:124 [View Article]
    [Google Scholar]
  27. Matsuda K, Tsuji H, Asahara T, Kado Y, Nomoto K. Sensitive quantitative detection of commensal bacteria by rRNA-targeted reverse transcription-PCR. Appl Environ Microbiol 2007; 73:32–39 [View Article]
    [Google Scholar]
  28. Bassetti M, Vena A, Croxatto A, Righi E, Guery B. How to manage Pseudomonas aeruginosa infections. Drugs Context 2018; 7:1–18 [View Article]
    [Google Scholar]
  29. Freschi L, Vincent AT, Jeukens J, Emond-Rheault J-G, Kukavica-Ibrulj I et al. The Pseudomonas aeruginosa pan-genome provides new insights on its population structure, horizontal gene transfer, and pathogenicity. Genome Biol Evol 2019; 11:109–120 [View Article]
    [Google Scholar]
  30. Choi Y, Sims GE, Murphy S, Miller JR, Chan AP. Predicting the functional effect of amino acid substitutions and indels. PLoS One 2012; 7:e46688 [View Article]
    [Google Scholar]
  31. Camacho C, Coulouris G, Avagyan V, Ma N, Papadopoulos J et al. BLAST+: architecture and applications. BMC Bioinformatics 2009; 10:421 [View Article]
    [Google Scholar]
  32. IBM CR. IBM SPSS Statistics for Windows, Version 25.0. Armonk, NY: IBM Corp; 2017
    [Google Scholar]
  33. Sousa AM, Pereira MO. Pseudomonas aeruginosa diversification during infection development in cystic fibrosis lungs-a review. Pathogens 2014; 3:680–703 [View Article]
    [Google Scholar]
  34. López-Causapé C, Cabot G, Del Barrio-Tofiño E, Oliver A. The versatile mutational resistome of Pseudomonas aeruginosa . Front Microbiol 2018; 9:685 [View Article]
    [Google Scholar]
  35. Rehman A, Patrick WM, Lamont IL. Mechanisms of ciprofloxacin resistance in Pseudomonas aeruginosa: new approaches to an old problem. J Med Microbiol 2019; 68:1–10 [View Article]
    [Google Scholar]
  36. Breidenstein EBM, de la Fuente-Núñez C, Hancock REW. Pseudomonas aeruginosa: all roads lead to resistance. Trends Microbiol 2011; 19:419–426 [View Article]
    [Google Scholar]
  37. Chávez-Jacobo VM, Hernández-Ramírez KC, Romo-Rodríguez P, Pérez-Gallardo RV, Campos-García J et al. CrpP Is a novel ciprofloxacin-modifying enzyme encoded by the Pseudomonas aeruginosa pUM505 plasmid. Antimicrob Agents Chemother 2018; 62:e02629-17 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.001085
Loading
/content/journal/jmm/10.1099/jmm.0.001085
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

EXCEL
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error