1887

Abstract

. Biochemical and molecular methods were used to identify 100 lactobacilli isolated from rectal swabs. Among these, ssp. LP5 and LP9 showed significant antibacterial activity against and . Accordingly, characterization of their bacteriocins, BacLP5 and BacLP9, was conducted to obtain information on their kinetic production, sensitivity to chemico-physical parameters and molecular weight. To investigate the possible use of the two strains as probiotics, their gastrointestinal resistance, cellular adhesiveness and sensitivity to antibiotics were also studied.

. The obtained data show that BacLP5 and BacLP9 most likely belong to class II bacteriocins and both have a molecular weight of approximately 3 kDa. The production of BacLP5 and BacLP9 started after 4 h (40 and 80 AU ml), respectively. Both of the strains survived gastric and intestinal juices well and showed adhesive capability on HEp-2 cells.

. Due to their peculiar antimicrobial characteristics, ssp. LP5 and LP9 are suitable for use in the treatment of vaginal disorders, through both oral and transvaginal administration.

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2019-09-01
2024-12-06
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References

  1. Allsworth JE, Peipert JF. Severity of bacterial vaginosis and the risk of sexually transmitted infection. Am J Obstet Gynecol 2011; 205:113.e1–11113 [View Article]
    [Google Scholar]
  2. Denney JM, Culhane JF. Bacterial vaginosis: a problematic infection from both a perinatal and neonatal perspective. Semin Fetal Neonatal Med 2009; 14:200–203 [View Article]
    [Google Scholar]
  3. Ravel J, Gajer P, Abdo Z, Schneider GM, Koenig SSK et al. Vaginal microbiome of reproductive-age women. Proc Natl Acad Sci USA 2011; 108:4680–4687 [View Article]
    [Google Scholar]
  4. Srinivasan S, Liu C, Mitchell CM, Fiedler TL, Thomas KK et al. Temporal variability of human vaginal bacteria and relationship with bacterial vaginosis. PLoS One 2010; 5:e10197 [View Article]
    [Google Scholar]
  5. Walter J. Ecological role of lactobacilli in the gastrointestinal tract: implications for fundamental and biomedical research. Appl Environ Microbiol 2008; 74:4985–4996 [View Article]
    [Google Scholar]
  6. Walter J, Tannock GW, Tilsala-Timisjarvi A, Rodtong S, Loach DM et al. Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers. Appl Environ Microbiol 2000; 66:297–303 [View Article]
    [Google Scholar]
  7. Forestier C, De Champs C, Vatoux C, Joly B. Probiotic activities of Lactobacillus casei rhamnosus: in vitro adherence to intestinal cells and antimicrobial properties. Res Microbiol 2001; 152:167–173 [View Article]
    [Google Scholar]
  8. Allain T, Mansour NM, Bahr MMA, Martin R, Florent I et al. A new lactobacilli in vivo expression system for the production and delivery of heterologous proteins at mucosal surfaces. FEMS Microbiol Lett 2016; 363:fnw117–10 [View Article]
    [Google Scholar]
  9. Gänzle MG, Hertel C, van der Vossen JM, Hammes WP. Effect of bacteriocin-producing lactobacilli on the survival of Escherichia coli and Listeria in a dynamic model of the stomach and the small intestine. Int J Food Microbiol 1999; 48:21–35 [View Article]
    [Google Scholar]
  10. De Gregorio PR, Juárez Tomás MS, Leccese Terraf MC, Nader-Macías MEF. In vitro and in vivo effects of beneficial vaginal lactobacilli on pathogens responsible for urogenital tract infections. J Med Microbiol 2014; 63:685–696 [View Article]
    [Google Scholar]
  11. Davoodabadi A, Soltan Dallal MM, Lashani E, Tajabadi Ebrahimi M. Antimicrobial activity of lactobacillus spp. isolated from fecal flora of healthy breast-fed infants against diarrheagenic Escherichia coli . Jundishapur J Microbiol 2015; 8:e27852 [View Article]
    [Google Scholar]
  12. Jara S, Sánchez M, Vera R, Cofré J, Castro E. The inhibitory activity of Lactobacillus spp. isolated from breast milk on gastrointestinal pathogenic bacteria of nosocomial origin. Anaerobe 2011; 17:474–477 [View Article]
    [Google Scholar]
  13. Sabia C, Anacarso I, Bergonzini A, Gargiulo R, Sarti M et al. Detection and partial characterization of a bacteriocin-like substance produced by Lactobacillus fermentum CS57 isolated from human vaginal secretions. Anaerobe 2014; 26:41–45 [View Article]
    [Google Scholar]
  14. Tannock GW. The bowel microflora: an important source of urinary tract pathogens. World J Urol 1999; 17:339–344 [View Article]
    [Google Scholar]
  15. Hilton E, Isenberg HD, Alperstein P, France K, Borenstein MT. Ingestion of yogurt containing Lactobacillus acidophilus as prophylaxis for candidal vaginitis. Ann Intern Med 1992; 116:353–357 [View Article]
    [Google Scholar]
  16. Antonio MAD, Rabe LK, Hillier SL. Colonization of the rectum by Lactobacillus species and decreased risk of bacterial vaginosis. J Infect Dis 2005; 192:394–398 [View Article]
    [Google Scholar]
  17. Lakshminarayanan B, Guinane CM, O'Connor PM, Coakley M, Hill C et al. Isolation and characterization of bacteriocin-producing bacteria from the intestinal microbiota of elderly Irish subjects. J Appl Microbiol 2013; 114:886–898 [View Article]
    [Google Scholar]
  18. Saarela M, Mogensen G, Fondén R, Mättö J, Mattila-Sandholm T et al. Functional and technological properties. J. Biotechnol 2000; 84:197–215
    [Google Scholar]
  19. Pendharkar S, Brandsborg E, Hammarström L, Marcotte H, Larsson P-G. Vaginal colonisation by probiotic lactobacilli and clinical outcome in women conventionally treated for bacterial vaginosis and yeast infection. BMC Infect Dis 2015; 15:255 [View Article]
    [Google Scholar]
  20. Reid G, Bruce AW, Fraser N, Heinemann C, Owen J et al. Oral probiotics can resolve urogenital infections. FEMS Immunol Med Microbiol 2001; 30:49–52 [View Article]
    [Google Scholar]
  21. Reid G, Charbonneau D, Erb J, Kochanowski B. Oral use of Lactobacillus rhamnosus GR-1 and L. fermentum RC-14 signicantly alters vaginal flora: randomized, placebo-controlled trial in 64 healthy women. FEMS Immunol Med Microbiol 2003; 3:131–134
    [Google Scholar]
  22. Mastromarino P, Brigidi P, Macchia S, Maggi L, Pirovano F et al. Characterization and selection of vaginal Lactobacillus strains for the preparation of vaginal tablets. J Appl Microbiol 2002; 93:884–893 [View Article]
    [Google Scholar]
  23. Anukam K, Osazuwa E, Ahonkhai I, Ngwu M, Osemene G et al. Augmentation of antimicrobial metronidazole therapy of bacterial vaginosis with oral probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14: randomized, double-blind, placebo controlled trial. Microbes Infect 2006; 8:1450–1454 [View Article]
    [Google Scholar]
  24. Ya W, Reifer C, Miller LE. Efficacy of vaginal probiotic capsules for recurrent bacterial vaginosis: a double-blind, randomized, placebo-controlled study. Am J Obstet Gynecol 2010; 203:120.e1–12120 [View Article]
    [Google Scholar]
  25. Vujic G, Jajac Knez A, Despot Stefanovic V, Kuzmic Vrbanovic V. Efficacy of orally applied probiotic capsules for bacterial vaginosis and other vaginal infections: a double-blind, randomized, placebo-controlled study. Eur J Obstet Gynecol Reprod Biol 2013; 168:75–79 [View Article]
    [Google Scholar]
  26. Heczko PB, Tomusiak A, Adamski P, Jakimiuk AJ, Stefański G et al. Supplementation of standard antibiotic therapy with oral probiotics for bacterial vaginosis and aerobic vaginitis: a randomised, double-blind, placebo-controlled trial. BMC Womens Health 2015; 15:115 [View Article]
    [Google Scholar]
  27. Tan Z, Pang H, Duan Y, Qin G, Cai Y. 16S ribosomal DNA analysis and characterization of lactic acid bacteria associated with traditional Tibetan Qula cheese made from yak milk. Anim Sci J 2010; 81:706–713 [View Article]
    [Google Scholar]
  28. Kekessy DA, Piguet JD. New method for detecting bacteriocin production. Appl Microbiol 1970; 2:282–283
    [Google Scholar]
  29. Rogers AM, Montville TJ. Improved agar diffusion assay for nisin quantification. Food Biotechnol 1991; 5:161–168 [View Article]
    [Google Scholar]
  30. Hernández D, Cardell E, Zárate V. Antimicrobial activity of lactic acid bacteria isolated from Tenerife cheese: initial characterization of plantaricin TF711, a bacteriocin-like substance produced by Lactobacillus plantarum TF711. J Appl Microbiol 2005; 99:77–84 [View Article]
    [Google Scholar]
  31. Zárate G, Chaia AP, González S, Oliver G. Viability and beta-galactosidase activity of dairy propionibacteria subjected to digestion by artificial gastric and intestinal fluids. J Food Prot 2000; 63:1214–1221 [View Article]
    [Google Scholar]
  32. Grey PA, Kirov SM. Adherence to IlEp-2 ceils, and enteropathogenic potential of Aeromonas spp. Epidemiol Infect 1993; 11:279–287
    [Google Scholar]
  33. CLSI Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard. CLSI document M07-A9, 9th ed. Wayn PA: Clinical and Laboratory Standards Institute; 2015
    [Google Scholar]
  34. European Food Safety Authority Guidance on the assessment of bacterial susceptibility to antimicrobials of human and veterinary importance. EFSA J 2012; 10:2740
    [Google Scholar]
  35. Tolinački M, Kojić M, Lozo J, Terzić-Vidojević A, Topisirovic L et al. Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9. Arch biol sci 2010; 62:889–899 [View Article]
    [Google Scholar]
  36. Guidone A, Zotta T, Ross RP, Stanton C, Rea MC et al. Functional properties of Lactobacillus plantarum strains: a multivariate screening study. LWT - Food Science and Technology 2014; 56:69–76 [View Article]
    [Google Scholar]
  37. Roos S, Engstrand L, Jonsson H. Lactobacillus gastricus sp. nov., Lactobacillus antri sp. nov., Lactobacillus kalixensis sp. nov. and Lactobacillus ultunensis sp. nov., isolated from human stomach mucosa. Int J Syst Evol Microbiol 2005; 55:77–82 [View Article]
    [Google Scholar]
  38. Delgado S, Cabrera-Rubio R, Mira A, Suárez A, Mayo B. Microbiological survey of the human gastric ecosystem using culturing and pyrosequencing methods. Microb Ecol 2013; 65:763–772 [View Article]
    [Google Scholar]
  39. Ryan KA, Jayaraman T, Daly P, Canchaya C, Curran S et al. Isolation of lactobacilli with probiotic properties from the human stomach. Lett Appl Microbiol 2008; 47:269–274 [View Article]
    [Google Scholar]
  40. de Klerk N, Maudsdotter L, Gebreegziabher H, Saroj SD, Eriksson B et al. Lactobacilli reduce Helicobacter pylori attachment to host gastric epithelial cells by inhibiting adhesion gene expression. Infect Immun 2016; 84:1526–1535 [View Article]
    [Google Scholar]
  41. Klaenhammer TR. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol Rev 1993; 12:39–85 [View Article]
    [Google Scholar]
  42. Nes IF, Diep DB, Håvarstein LS, Brurberg MB, Eijsink V et al. Biosynthesis of bacteriocins in lactic acid bacteria. Antonie Van Leeuwenhoek 1996; 70:113–128 [View Article]
    [Google Scholar]
  43. Mirkovic N, Polovic N, Vukotic G, Jovcic B, Miljkovic M et al. Lactococcus lactis LMG2081 produces two bacteriocins, a nonlantibiotic and a novel lantibiotic. Appl Environ Microbiol 2016; 82:2555–2562 [View Article]
    [Google Scholar]
  44. Ming L, Zhang Q, Yang L, Huang JA. Comparison of antibacterial effects between antimicrobial peptide and bacteriocins isolated from Lactobacillus plantarum on three common pathogenic bacteria. Int J Clin Exp Med 2015; 8:5806–5811
    [Google Scholar]
  45. European Food Safety Authority The maintenance of the list of QPs microorganisms intentionally added to food or feed. Efsa J 2008; 923:1–48
    [Google Scholar]
  46. Witkin SS. The vaginal microbiome, vaginal anti-microbial defence mechanisms and the clinical challenge of reducing infection-related preterm birth. BJOG: Int J Obstet Gy 2015; 122:213–218 [View Article]
    [Google Scholar]
  47. Cribby S, Taylor M, Reid G. Vaginal microbiota and the use of probiotics. Interdiscip Perspect Infect Dis 2008; 2008:1–9 [View Article]
    [Google Scholar]
  48. Macklaim JM, Clemente JC, Knight R, Gloor GB, Reid G. Changes in vaginal microbiota following antimicrobial and probiotic therapy. Microb Ecol Health Dis 2015; 26:27799 [View Article]
    [Google Scholar]
  49. Kim J-M, Park YJ. Probiotics in the prevention and treatment of postmenopausal vaginal infections: review article. J Menopausal Med 2017; 23:139–145 [View Article]
    [Google Scholar]
  50. Mogha VK, Prajapati JB. Probiotics for the treatment of bacterial vaginosis. J Med Microbiol 2016; 27:87–94
    [Google Scholar]
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