1887

Abstract

. Biochemical and molecular methods were used to identify 100 lactobacilli isolated from rectal swabs. Among these, ssp. LP5 and LP9 showed significant antibacterial activity against and . Accordingly, characterization of their bacteriocins, BacLP5 and BacLP9, was conducted to obtain information on their kinetic production, sensitivity to chemico-physical parameters and molecular weight. To investigate the possible use of the two strains as probiotics, their gastrointestinal resistance, cellular adhesiveness and sensitivity to antibiotics were also studied.

. The obtained data show that BacLP5 and BacLP9 most likely belong to class II bacteriocins and both have a molecular weight of approximately 3 kDa. The production of BacLP5 and BacLP9 started after 4 h (40 and 80 AU ml), respectively. Both of the strains survived gastric and intestinal juices well and showed adhesive capability on HEp-2 cells.

. Due to their peculiar antimicrobial characteristics, ssp. LP5 and LP9 are suitable for use in the treatment of vaginal disorders, through both oral and transvaginal administration.

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2019-09-01
2019-09-18
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