1887

Abstract

Methicillin-resistant (MRSA) can be classified into hospital-acquired MRSA (HA-MRSA) and community-associated MRSA (CA-MRSA), based on the associated epidemiological risk factors and their SCC types. We therefore studied the diversity and distribution of SCC elements among MRSA isolates in our region and also evaluated SCC typing as a tool for the classification of MRSA.

Two hundred isolates of MRSA obtained from various clinical specimens were included. The clinical and demographic details of the patients and the epidemiological risk factors for MRSA acquisition were documented. Multiplex PCR was optimized for all the major SCC types (I to V). Subtyping of SCC type IV (IVb, IVc, IVd, IVh) was carried out by simplex PCR.

Based on epidemiological criteria, CA-MRSA constituted 57  % (114/200) of the the test isolates and HA-MRSA made up 43  % (86/200). The predominant SCC type found in our study was type III (62%), followed by type V (52.5%) and type I (47.5%), while type II was carried by a single isolate. Of the 200 isolates, 118 carried multiple SCC types and 3 were non-typable.

The existence of multiple SCC types in individual MRSA isolates resulted in our inability to categorize many of these isolates as either CA-MRSA or HA-MRSA as defined by the SCC type criterion.

The major limitation of the study was that the SCC element of MRSA isolates exhibiting multiple types was not sequenced and hence this finding could not be confirmed.

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2019-05-01
2019-12-05
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