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Abstract

Purpose . This study evaluated in-house PCR testing for local identification of bacteria carrying the major carbapenemase genes (bla OXA-48-like, bla VIM, bla NDM, bla KPC and bla IMP).

Methodology . Carbapenemase-producing organisms (CPOs) isolated from patients managed in two tertiary care hospitals in Scotland from September 2014–January 2017 were investigated. A combination of chromogenic screening agar (ChromID CARBA SMART), a carbapenem hydrolysis test (Rapidec Carba NP) and in-house real-time PCR for the bla OXA-48-like, bla VIM, bla NDM, bla KPC and bla IMP genes were utilized. All isolates were sent to the AMRHAI reference unit for confirmatory testing.

Results . During the 29-month study period 39 CPO were isolated from 34 patients. The average turnaround time for a workflow involving phenotypic and molecular testing was 4.2 days. PCR had a sensitivity and specificity of 100 %. The most common carbapenemase genes were bla OXA-48-like (31%), bla VIM (23%) and bla NDM (20%). Resistance to antimicrobials other than beta-lactams was common; the most active agents were colistin, amikacin and fosfomycin. Twenty-seven patients were considered to be colonized (although CPO detection influenced empiric antimicrobials in five) and a CPO was implicated in infection in seven patients (bacteraemia in immunocompromised patients, n=2; surgical site infections, n=2; osteomyelitis in a patient with diabetes mellitus, n=1; and urinary tract infections, n=2). All patients survived infection.

Conclusion . In a lowincidence setting we demonstrate the efficacy of a combined local laboratory workflow for rapid detection of CPOs, incorporating phenotypic and molecular testing. In 7/34 patients the CPO was implicated as a pathogen and detection influenced antimicrobial decision-making in five colonized patients.

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/content/journal/jmm/10.1099/jmm.0.000931
2019-03-14
2019-08-18
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