1887

Abstract

We have cultured and phenotyped human adipose tissue-derived mesenchymal stem/stromal cells (AT MSCs) and inoculated these cultures with bacteria common to infected skin wounds, i.e. Staphylococcus aureus and Pseudomonas aeruginosa. Cell interactions were examined by scanning electron microscopy (SEM), whilst bacterial growth was measured by colony forming unit (c.f.u.) and biofilm assays. AT MSCs appeared to attach to the bacteria and to engulf S. aureus. Significantly fewer bacterial c.f.u. were present in AT MSC : bacterial co-cultures compared with bacteria cultured alone. Antibacterial activity, including an inhibition of P. aeruginosa biofilm formation, was observed when bacteria were treated with conditioned medium harvested from the AT MSC :  bacterial co-cultures, irrespective of the bacterial species to which the AT MSCs had been exposed to previously. Hence, we have demonstrated that AT MSCs inhibit the growth of two common bacterial species. This was associated with bacterial adhesion, potential engulfment or phagocytosis, and the secretion of antibacterial factors.

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2018-10-23
2024-12-04
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References

  1. Walter MN, Wright KT, Fuller HR, MacNeil S, Johnson WE. Mesenchymal stem cell-conditioned medium accelerates skin wound healing: an in vitro study of fibroblast and keratinocyte scratch assays. Exp Cell Res 2010; 316:1271–1281 [View Article][PubMed]
    [Google Scholar]
  2. Wright KT, El Masri W, Osman A, Roberts S, Chamberlain G et al. Bone marrow stromal cells stimulate neurite outgrowth over neural proteoglycans (CSPG), myelin associated glycoprotein and Nogo-A. Biochem Biophys Res Commun 2007; 354:559–566 [View Article][PubMed]
    [Google Scholar]
  3. Tan Y, Uchida K, Nakajima H, Guerrero AR, Watanabe S et al. Blockade of interleukin 6 signaling improves the survival rate of transplanted bone marrow stromal cells and increases locomotor function in mice with spinal cord injury. J Neuropathol Exp Neurol 2013; 72:980–993 [View Article][PubMed]
    [Google Scholar]
  4. Krasnodembskaya A, Song Y, Fang X, Gupta N, Serikov V et al. Antibacterial effect of human mesenchymal stem cells is mediated in part from secretion of the antimicrobial peptide LL-37. Stem Cells 2010; 28:2229–2238 [View Article][PubMed]
    [Google Scholar]
  5. Caplan AI, Correa D. The MSC: an injury drugstore. Cell Stem Cell 2011; 9:11–15 [View Article][PubMed]
    [Google Scholar]
  6. Maxson S, Lopez EA, Yoo D, Danilkovitch-Miagkova A, Leroux MA. Concise review: role of mesenchymal stem cells in wound repair. Stem Cells Transl Med 2012; 1:142–149 [View Article][PubMed]
    [Google Scholar]
  7. Salgado AJ, Gimble JM. Secretome of mesenchymal stem/stromal cells in regenerative medicine. Biochimie 2013; 95:2195 [View Article][PubMed]
    [Google Scholar]
  8. Johnson V, Webb T, Dow S. Activated mesenchymal stem cells amplify antibiotic activity against chronic Staphylococcus aureus infection (P5056). J Immunol 2013; 190:180.11
    [Google Scholar]
  9. Liew LJ, Ong HT, Dilley RJ. Isolation and culture of adipose-derived stromal cells from subcutaneous fat. In Rittié L. (editor) Fibrosis: Methods and Protocols New York, NY: Springer New York; 2017 pp. 193–203
    [Google Scholar]
  10. Ren H, Sang Y, Zhang F, Liu Z, Qi N et al. Comparative analysis of human mesenchymal stem cells from umbilical cord, dental pulp, and menstrual blood as sources for cell therapy. Stem Cells Int 2016; 2016:1–13 [View Article][PubMed]
    [Google Scholar]
  11. Lee SH, Jin SY, Song JS, Seo KK, Cho KH. Paracrine effects of adipose-derived stem cells on keratinocytes and dermal fibroblasts. Ann Dermatol 2012; 24:136–143 [View Article][PubMed]
    [Google Scholar]
  12. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006; 8:315–317 [View Article][PubMed]
    [Google Scholar]
  13. Kohli N, Wright KT, Sammons RL, Jeys L, Snow M et al. An in vitro comparison of the incorporation, growth, and chondrogenic potential of human bone marrow versus adipose tissue mesenchymal stem cells in clinically relevant cell scaffolds used for cartilage repair. Cartilage 2015; 6:252–263 [View Article][PubMed]
    [Google Scholar]
  14. Miles AA, Misra SS, Irwin JO. The estimation of the bactericidal power of the blood. J Hyg 1938; 38:732–749 [View Article][PubMed]
    [Google Scholar]
  15. Wei Q, Ma LZ. Biofilm matrix and its regulation in Pseudomonas aeruginosa. Int J Mol Sci 2013; 14:20983–21005 [View Article][PubMed]
    [Google Scholar]
  16. Sung DK, Chang YS, Sung SI, Yoo HS, Ahn SY et al. Antibacterial effect of mesenchymal stem cells against Escherichia coli is mediated by secretion of beta- defensin- 2 via toll- like receptor 4 signalling. Cell Microbiol 2016; 18:424–436 [View Article][PubMed]
    [Google Scholar]
  17. Rashedi I, Gómez-Aristizábal A, Wang XH, Viswanathan S, Keating A et al. TLR3 or TLR4 activation enhances mesenchymal stromal cell-mediated treg induction via notch signaling. Stem Cells 2017; 35:265–275 [View Article][PubMed]
    [Google Scholar]
  18. Caplan AI, Dennis JE. Mesenchymal stem cells as trophic mediators. J Cell Biochem 2006; 98:1076–1084 [View Article][PubMed]
    [Google Scholar]
  19. Spees JL, Lee RH, Gregory CA. Mechanisms of mesenchymal stem/stromal cell function. Stem Cell Res Ther 2016; 7:125 [View Article][PubMed]
    [Google Scholar]
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