1887

Abstract

The mcr-1 gene encodes a phosphoethanolamine transferase, which confers resistance to colistin by transferring phosphoethanolamine to lipid A. This study describes the emergence of a colistin- and carbapenem-resistant clinical isolate of Escherichia coli harbouring mcr-1 and bla NDM-5 genes, located on 90 and 150 Kb plasmids, respectively. The isolate belonged to ST132. This is the first report of a clinical isolate in Japan co-harbouring mcr-1 and bla NDM-5.

Keyword(s): colistin resistance , CRE , Mcr-1 and NDM-5
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/content/journal/jmm/10.1099/jmm.0.000793
2018-07-04
2019-09-15
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References

  1. Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis 2016;16:161–168 [CrossRef][PubMed]
    [Google Scholar]
  2. Tada T, Uechi K, Nakasone I, Shimada K, Nakamatsu M et al. Emergence of a colistin-resistant Escherichia coli clinical isolate harboring mcr-1 in Japan. Int J Infect Dis 2017;63:21–22 [CrossRef][PubMed]
    [Google Scholar]
  3. Yong D, Toleman MA, Giske CG, Cho HS, Sundman K et al. Characterization of a new metallo-beta-lactamase gene, blaNDM-1, and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrob Agents Chemother 2009;53:5046–5054 [CrossRef][PubMed]
    [Google Scholar]
  4. Nordmann P, Poirel L. The difficult-to-control spread of carbapenemase producers among Enterobacteriaceae worldwide. Clin Microbiol Infect 2014;20:821–830 [CrossRef][PubMed]
    [Google Scholar]
  5. Khan AU, Maryam L, Zarrilli R. Structure, genetics and worldwide spread of New Delhi metallo-β-lactamase (NDM): a threat to public health. BMC Microbiol 2017;17:101 [CrossRef][PubMed]
    [Google Scholar]
  6. Poirel L, Jayol A, Nordmann P. Polymyxins: antibacterial activity, susceptibility testing, and resistance mechanisms encoded by plasmids or chromosomes. Clin Microbiol Rev 2017;30:557–596 [CrossRef][PubMed]
    [Google Scholar]
  7. Tada T, Miyoshi-Akiyama T, Shimada K, Kirikae T. Biochemical analysis of metallo-β-lactamase NDM-3 from a multidrug-resistant Escherichia coli strain isolated in Japan. Antimicrob Agents Chemother 2014;58:3538–3540 [CrossRef][PubMed]
    [Google Scholar]
  8. Zhang Y, Liao K, Gao H, Wang Q, Wang X et al. Decreased fitness and virulence in ST10 Escherichia coli harboring blaNDM-5 and mcr-1 against a ST4981 strain with blaNDM-5. Front Cell Infect Microbiol 2017;7:242 [CrossRef]
    [Google Scholar]
  9. Li A, Yang Y, Miao M, Chavda KD, Mediavilla JR et al. Complete sequences of mcr-1-harboring plasmids from extended-spectrum-β-lactamase- and carbapenemase-producing Enterobacteriaceae. Antimicrob Agents Chemother 2016;60:4351–4354 [CrossRef][PubMed]
    [Google Scholar]
  10. Mediavilla JR, Patrawalla A, Chen L, Chavda KD, Mathema B et al. Colistin- and carbapenem-resistant Escherichia coli harboring mcr-1 and blaNDM-5, causing a complicated urinary tract infection in a patient from the United States. MBio 2016;7:e01191-16 [CrossRef][PubMed]
    [Google Scholar]
  11. Zheng B, Yu X, Xu H, Guo L, Zhang J et al. Complete genome sequencing and genomic characterization of two Escherichia coli strains co-producing MCR-1 and NDM-1 from bloodstream infection. Sci Rep 2017;7:17885 [CrossRef][PubMed]
    [Google Scholar]
  12. Lai CC, Chuang YC, Chen CC, Tang HJ. Coexistence of MCR-1 and NDM-9 in a clinical carbapenem-resistant Escherichia coli isolate. Int J Antimicrob Agents 2017;49:517–518 [CrossRef][PubMed]
    [Google Scholar]
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