1887

Abstract

The resistance/nodulation/cell division (RND) family multidrug efflux pump, OqxAB, has been identified as one of the leading mechanisms of plasmid-mediated quinolone resistance and has become increasingly prevalent among in recent years. However, AB genes have not yet been reported in isolates. The aim of the present study was to identify the AB genes and investigate their prevalence among from swine manure in China.

The AB genes were screened in 87 isolates by PCR. The transferability of the AB genes in was determined by conjugation experiments. The genetic environment of AB genes was investigated by cloning experiments, PCR mapping and sequencing.

A high prevalence (86.2 %) of olaquindox resistance was observed in and 98.9 % isolates exhibited multidrug-resistance phenotypes. The occurrence of A and B in was also high (79.3 and 65.5 %, respectively). Sequence analysis of the cloned fragment indicated that the AB cassette was linked to an incomplete Tn5 transposon containing and flanked by IS [ISAB-IS]. The AB–-positive transconjugant or transformant showed resistance or reduced susceptibility to enrofloxacin, ciprofloxacin, olaquindox, mequindox, florfenicol, neomycin and kanamycin.

This is the first time that the AB genes have been identified in from swine manure. The genetic linkage of AB– in has not been described before. The high prevalence of AB genes in suggests that it may constitute a reservoir for AB genes and pose a potential threat to public health.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000736
2018-06-01
2020-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jmm/67/6/733.html?itemId=/content/journal/jmm/10.1099/jmm.0.000736&mimeType=html&fmt=ahah

References

  1. Hansen LH, Sørensen SJ, Jørgensen HS, Jensen LB. The prevalence of the OqxAB multidrug efflux pump amongst olaquindox-resistant Escherichia coli in pigs. Microb Drug Resist 2005;11:378–382 [CrossRef][PubMed]
    [Google Scholar]
  2. Kim HB, Wang M, Park CH, Kim EC, Jacoby GA et al. oqxAB encoding a multidrug efflux pump in human clinical isolates of Enterobacteriaceae. Antimicrob Agents Chemother 2009;53:3582–3584 [CrossRef][PubMed]
    [Google Scholar]
  3. Li L, Liao X, Yang Y, Sun J, Li L et al. Spread of oqxAB in Salmonella enterica serotype Typhimurium predominantly by IncHI2 plasmids. J Antimicrob Chemother 2013;68:2263–2268 [CrossRef][PubMed]
    [Google Scholar]
  4. Perez F, Rudin SD, Marshall SH, Coakley P, Chen L et al. OqxAB, a quinolone and olaquindox efflux pump, is widely distributed among multidrug-resistant Klebsiella pneumoniae isolates of human origin. Antimicrob Agents Chemother 2013;57:4602–4603 [CrossRef][PubMed]
    [Google Scholar]
  5. Park KS, Kim MH, Park TS, Nam YS, Lee HJ et al. Prevalence of the plasmid-mediated quinolone resistance genes, aac(6')-Ib-cr, qepA, and oqxAB in clinical isolates of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae in Korea. Ann Clin Lab Sci 2012;42:191–197[PubMed]
    [Google Scholar]
  6. Rodríguez-Martínez JM, Díaz de Alba P, Briales A, Machuca J, Lossa M et al. Contribution of OqxAB efflux pumps to quinolone resistance in extended-spectrum-β-lactamase-producing Klebsiella pneumoniae. J Antimicrob Chemother 2013;68:68–73 [CrossRef][PubMed]
    [Google Scholar]
  7. Wong MH, Chen S. First detection of oqxAB in Salmonella spp. isolated from food. Antimicrob Agents Chemother 2013;57:658–660 [CrossRef][PubMed]
    [Google Scholar]
  8. Rodríguez-Martínez JM, Díaz de Alba P, Briales A, Machuca J, Lossa M et al. Contribution of OqxAB efflux pumps to quinolone resistance in extended-spectrum-β-lactamase-producing Klebsiella pneumoniae. J Antimicrob Chemother 2013;68:68–73 [CrossRef][PubMed]
    [Google Scholar]
  9. Yuan J, Xu X, Guo Q, Zhao X, Ye X et al. Prevalence of the oqxAB gene complex in Klebsiella pneumoniae and Escherichia coli clinical isolates. J Antimicrob Chemother 2012;67:1655–1659 [CrossRef][PubMed]
    [Google Scholar]
  10. Zhao J, Chen Z, Chen S, Deng Y, Liu Y et al. Prevalence and dissemination of oqxAB in Escherichia coli isolates from animals, farmworkers, and the environment. Antimicrob Agents Chemother 2010;54:4219–4224 [CrossRef][PubMed]
    [Google Scholar]
  11. Wong MH, Chan EW, Liu LZ, Chen S. PMQR genes oqxAB and aac(6')Ib-cr accelerate the development of fluoroquinolone resistance in Salmonella typhimurium. Front Microbiol 2014;5:521 [CrossRef][PubMed]
    [Google Scholar]
  12. Zhong X, Xu H, Chen D, Zhou H, Hu X et al. First emergence of acrAB and oqxAB mediated tigecycline resistance in clinical isolates of Klebsiella pneumoniae pre-dating the use of tigecycline in a Chinese hospital. PLoS One 2014;9:e115185 [CrossRef][PubMed]
    [Google Scholar]
  13. Wang LH, Dh L, Zhang H, Yang B, Wei WC et al. Distribution of plasmid mediated quinlone resistant genes in Staphylococcus aureus isolated from animals. Chin J Vet Sci 2014;34:606–611
    [Google Scholar]
  14. Wong MH, Chan EW, Chen S. Evolution and dissemination of OqxAB-like efflux pumps, an emerging quinolone resistance determinant among members of Enterobacteriaceae. Antimicrob Agents Chemother 2015;59:3290–3297 [CrossRef][PubMed]
    [Google Scholar]
  15. Chow JW, Kak V, You I, Kao SJ, Petrin J et al. Aminoglycoside resistance genes aph(2")-Ib and aac(6')-Im detected together in strains of both Escherichia coli and Enterococcus faecium. Antimicrob Agents Chemother 2001;45:2691–2694 [CrossRef][PubMed]
    [Google Scholar]
  16. Wang YB, Zhang X, Ql H, Meng ZB, Chen LY et al. Studies on biological characteristics of Enterococcus faecalis caused by piglet’s arthritis. J Henan AgricUniv 2011;45:183–187
    [Google Scholar]
  17. CLSI Performance Standards for Antimicrobial Susceptibility Testing, CLSI Document M100-S26. Wayne, PA: Clinical and Laboratory Standards Institute; 2016
    [Google Scholar]
  18. CLSI Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria isolated from Animals, Approved Standard-Fourth Edition and Supplement, CLSI Documents VET01-A4E and VET01-S2E. Wayne, PA: Clinical and Laboratory Standards Institute; 2015
    [Google Scholar]
  19. Al-Badah AS, Ibrahim ASS, Al-Salamah AA, Ibrahim SSS. Clonal diversity and antimicrobial resistance of Enterococcus faecalis isolated from endodontic infections. Electron J Biotechn 2015;18:175–180 [CrossRef]
    [Google Scholar]
  20. Werner G, Willems RJ, Hildebrandt B, Klare I, Witte W. Influence of transferable genetic determinants on the outcome of typing methods commonly used for Enterococcus faecium. J Clin Microbiol 2003;41:1499–1506 [CrossRef][PubMed]
    [Google Scholar]
  21. Norman A, Hansen LH, She Q, Sørensen SJ. Nucleotide sequence of pOLA52: a conjugative IncX1 plasmid from Escherichia coli which enables biofilm formation and multidrug efflux. Plasmid 2008;60:59–74 [CrossRef][PubMed]
    [Google Scholar]
  22. Cheng L, Cao XL, Zhang ZF, Ning MZ, Xu XJ et al. Clonal dissemination of KPC-2 producing Klebsiella pneumoniae ST11 clone with high prevalence of oqxAB and rmtB in a tertiary hospital in China: results from a 3-year period. Ann Clin Microbiol Antimicrob 2016;15:1 [CrossRef][PubMed]
    [Google Scholar]
  23. Martínez-Martínez L, Pascual A, Jacoby GA. Quinolone resistance from a transferable plasmid. Lancet 1998;351:797–799 [CrossRef][PubMed]
    [Google Scholar]
  24. Robicsek A, Jacoby GA, Hooper DC. The worldwide emergence of plasmid-mediated quinolone resistance. Lancet Infect Dis 2006;6:629–640 [CrossRef][PubMed]
    [Google Scholar]
  25. Rodríguez-Martínez JM, Cano ME, Velasco C, Martínez-Martínez L, Pascual A. Plasmid-mediated quinolone resistance: an update. J Infect Chemother 2011;17:149–182 [CrossRef][PubMed]
    [Google Scholar]
  26. Strahilevitz J, Jacoby GA, Hooper DC, Robicsek A. Plasmid-mediated quinolone resistance: a multifaceted threat. Clin Microbiol Rev 2009;22:664–689 [CrossRef][PubMed]
    [Google Scholar]
  27. Campos J, Mourão J, Marçal S, Machado J, Novais C et al. Clinical Salmonella Typhimurium ST34 with metal tolerance genes and an IncHI2 plasmid carrying oqxAB-aac(6')-Ib-cr from Europe. J Antimicrob Chemother 2016;71:843–845 [CrossRef][PubMed]
    [Google Scholar]
  28. Liu BT, Wang XM, Liao XP, Sun J, Zhu HQ et al. Plasmid-mediated quinolone resistance determinants oqxAB and aac(6')-Ib-cr and extended-spectrum β-lactamase gene blaCTX-M-24 co-located on the same plasmid in one Escherichia coli strain from China. J Antimicrob Chemother 2011;66:1638–1639 [CrossRef][PubMed]
    [Google Scholar]
  29. Liu BT, Yang QE, Li L, Sun J, Liao XP et al. Dissemination and characterization of plasmids carrying oqxAB-blaCTX-M genes in Escherichia coli isolates from food-producing animals. PLoS One 2013;8:e73947 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000736
Loading
/content/journal/jmm/10.1099/jmm.0.000736
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error