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Abstract

Catheter-related bloodstream infections (CRBSIs) are among the most common hospital-acquired infections. We aimed to survey methicillin resistance, biofilm production and susceptibility to vancomycin, linezolid and other antibiotics for staphylococci isolated from CRBSIs.

Fifty-eight isolates [20  and 38 coagulase-negative staphylococci (CoNS; 20 , nine , three , two and four )] were tested for methicillin resistance by cefoxitin disk diffusion and detection of the gene by PCR; biofilm-forming ability using Congo red agar and tissue culture plate methods; susceptibility to ciprofloxacin, clindamycin, cotrimoxazole, erythromycin, gentamicin, linezolid, rifampicin and tetracycline; and MIC determination for vancomycin.

Cefoxitin resistance was detected among 40 % (8/20) isolates, 70 % (14/20) isolates and 16.7 % (3/18) of other CoNS, although the gene was detected in 45 % (9/20) isolates, 35 % (7/20) isolates and 16.7 % (3/18) of other CoNS. Biofilm-forming ability ranged from 45 to 75 %. Methicillin-resistant and other CoNS were considered to be more virulent than methicillin-resistant due to the higher biofilm forming abilities of the former. All tested isolates exhibited 100 % sensitivity to vancomycin and linezolid, irrespective of their methicillin resistance or biofilm-forming ability. Rifampicin showed overall sensitivity of 75.9 %. Varying degrees of multi-resistance were found for the other antibiotics.

Vancomycin, linezolid and rifampicin could be used effectively against methicillin-resistant staphylococci isolated from CRBSIs.

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2017-06-01
2024-10-06
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