1887

Abstract

It is known that the (or ) gene encoding uridine 5′-(beta-1-threo-pentapyranosyl-4-ulose diphosphate) aminotransferase plays a critical role in colistin resistance in through the addition of 4-amino-4-deoxy--arabinose (-Ara4N) to lipid A. In this study, we attempted to obtain a colistin-resistant mutant from an -deleted mutant through exposure to colistin.

We constructed an deletion mutant (P5Δ:: ) from a colistin-susceptible strain (P5) by allelic replacement mutagenesis, and colistin-resistant mutants were selected using P5 and P5Δ:: . The growth rate, lipid A structure, biofilm-forming activity and cell viability in diverse stressful conditions (osmotic, oxidative, acidic and heat stress) were investigated. Expression of and was evaluated by qRT-PCR.

An deletion mutant was shown to develop colistin resistance through the addition of -Ara4N to lipid A, despite a low survival rate (over 1000-fold lower than that of the wild-type strain) in the media with colistin. Two colistin-resistant mutants showed higher survival rates than colistin-susceptible strains against 5 % NaCl. In the presence of acidic and heat stress, P5Δ:: -Cst exhibited higher survival rates during conditions of 1 % HCl and 42 °C than the other strains. Both and genes were overexpressed significantly in both colistin-resistant mutants, but and genes were not.

We revealed that colistin resistance could be developed despite deletion in through the addition of -Ara4N to lipid A, which was accompanied by diverse physiological changes.

Keyword(s): arnB , colistin and lipid A
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2017-06-01
2020-12-02
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