1887

Abstract

A recently identified colistin resistance gene, , has been reported in many countries. In this study, we established a new real-time PCR method to detect it.

We used a real-time PCR method to detect the gene in a variety of isolates and faecal samples from 20 provinces and municipal cities in China.

Of the 2330 isolates (from 10 species) screened, 54 (2.3 %) isolates were positive for . All of the -positive isolates that were identified belonged to strains, among which 9, 1, and 44 were identified as enteropathogenic , enteroadherent , and non-pathogenic , respectively. The majority of the -positive isolates were obtained from farm animals from eight provinces and municipal cities across China. A total of 337 faecal samples, including 229 human and 108 pet animal faecal samples, were also screened for the gene. Of the 337 samples analyzed, six and eight human and pet animal faecal samples were positive for the gene, respectively.

The data demonstrate that the gene is highly prevalent in human and animal populations in China. This occurrence suggests that active surveillance of the gene is imperative in curtailing its spread.

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/content/journal/jmm/10.1099/jmm.0.000425
2017-02-01
2020-08-04
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