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Abstract

Community-associated meticillin-resistant (CA-MRSA) have emerged globally and have been associated with more severe disease than healthcare-associated MRSA (HA-MRSA). The purpose of this study was to determine whether laboratory measures of virulence can distinguish dominant CA-MRSA clones from HA-MRSA clones. We compared the production of phenol-soluble modulins (PSMs) and ability to kill caterpillars for a range of CA- and HA-MRSA strains. Twenty-two HA-MRSA strains [ST22-IV (EMRSA-15), ST36-II (EMRSA-16) and ST239-III] and 26 CA-MRSA strains [ST1-IV (PVL+ USA400), ST1-IV (PVL–), ST8-IV (USA300), ST22-IV (PVL+), ST30-IV, ST59-IV and ST80-IV] were analysed. PSM production was measured using and compared using -tests and ANOVA. A (caterpillar) pathogenicity model was performed, and differences were compared using survival analysis and the log-rank test. There was no significant difference in overall PSM production between HA and CA strains (=0.090), but there was significant variation between clones (=0.003). caterpillar killing varied significantly by clone (<0.001), and overall killing was greater for HA compared with CA clones (=0.007). The increased acute virulence phenotype of CA-MRSA clones in humans is not associated with increased PSM production or increased killing in an caterpillar pathogenicity model.

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2016-12-16
2024-12-09
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References

  1. Anderson L., Hunter C. L. 2006; Quantitative mass spectrometric multiple reaction monitoring assays for major plasma proteins. Mol Cell Proteomics 5:573588 [View Article][PubMed]
    [Google Scholar]
  2. Boakes E., Kearns A. M., Ganner M., Perry C., Warner M., Hill R. L., Ellington M. J. 2011; Molecular diversity within clonal complex 22 methicillin-resistant Staphylococcus aureus encoding Panton-Valentine leukocidin in England and Wales. Clin Microbiol Infect 17:140145 [View Article][PubMed]
    [Google Scholar]
  3. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72:248254 [View Article][PubMed]
    [Google Scholar]
  4. Bubeck Wardenburg J., Palazzolo-Ballance A. M., Otto M., Schneewind O., DeLeo F. R., Bubeck W. J. 2008; Panton- Valentine leukocidin is not a virulence determinant in murine models of community-associated methicillin-resistant Staphylococcus aureus disease. J Infect Dis 198:11661170 [View Article][PubMed]
    [Google Scholar]
  5. Chambers H. F. 2005; Community-associated MRSA-resistance and virulence converge. N Engl J Med 352:14851487 [View Article][PubMed]
    [Google Scholar]
  6. Chambers H. F., Deleo F. R. 2009; Waves of resistance: Staphylococcus aureus in the antibiotic era. Nat Rev Microbiol 7:629641 [View Article][PubMed]
    [Google Scholar]
  7. Cheung G. Y., Wang R., Khan B. A., Sturdevant D. E., Otto M. 2011; Role of the accessory gene regulator agr in community-associated methicillin-resistant Staphylococcus aureus pathogenesis. Infect Immun 79:19271935 [View Article][PubMed]
    [Google Scholar]
  8. Chua K. Y., Seemann T., Harrison P. F., Monagle S., Korman T. M., Johnson P. D., Coombs G. W., Howden B. O., Davies J. K. et al. 2011; The dominant Australian community-acquired methicillin-resistant Staphylococcus aureus clone ST93-IV [2B] is highly virulent and genetically distinct. PLoS One 6:e25887 [View Article][PubMed]
    [Google Scholar]
  9. David M. Z., Daum R. S. 2010; Community-associated methicillin-resistant Staphylococcus aureus: epidemiology and clinical consequences of an emerging epidemic. Clin Microbiol Rev 23:616687 [View Article][PubMed]
    [Google Scholar]
  10. HPA 2008 Guidance on the diagnosis and management of PVL-associated Staphylococcus aureus infections (PVL-SA) in England.
  11. Joo H. S., Chan J. L., Cheung G. Y., Otto M. 2010; Subinhibitory concentrations of protein synthesis-inhibiting antibiotics promote increased expression of the agr virulence regulator and production of phenol-soluble modulin cytolysins in community-associated methicillin-resistant Staphylococcus aureus . Antimicrob Agents Chemother 54:49424944 [View Article][PubMed]
    [Google Scholar]
  12. Joo H. S., Cheung G. Y., Otto M. 2011; Antimicrobial activity of community-associated methicillin-resistant Staphylococcus aureus is caused by phenol-soluble modulin derivatives. J Biol Chem 286:89338940 [View Article][PubMed]
    [Google Scholar]
  13. Labandeira-Rey M., Couzon F., Boisset S., Brown E. L., Bes M., Benito Y., Barbu E. M., Vazquez V., Höök M. et al. 2007; Staphylococcus aureus Panton-Valentine leukocidin causes necrotizing pneumonia. Science 315:11301133 [View Article][PubMed]
    [Google Scholar]
  14. Li M., Diep B. A., Villaruz A. E., Braughton K. R., Jiang X., DeLeo F. R., Chambers H. F., Lu Y., Otto M. 2009; Evolution of virulence in epidemic community-associated methicillin-resistant Staphylococcus aureus . Proc Natl Acad Sci U S A 106:58835888 [View Article]
    [Google Scholar]
  15. Montgomery C. P., Boyle-Vavra S., Adem P. V., Lee J. C., Husain A. N., Clasen J., Daum R. S. 2008; Comparison of virulence in community-associated methicillin-resistant Staphylococcus aureus pulsotypes USA300 and USA400 in a rat model of pneumonia. J Infect Dis 198:561570 [View Article][PubMed]
    [Google Scholar]
  16. Naimi T. S., LeDell K. H., Como-Sabetti K., Borchardt S. M., Boxrud D. J., Etienne J., Johnson S. K., Vandenesch F., Fridkin S. et al. 2003; Comparison of community- and health care-associated methicillin-resistant Staphylococcus aureus infection. J Am Med Assoc 290:29762984 [View Article][PubMed]
    [Google Scholar]
  17. Otter J. A., French G. L. 2010; Molecular epidemiology of community-associated meticillin-resistant Staphylococcus aureus in Europe. Lancet Infect Dis 10:227239 [View Article][PubMed]
    [Google Scholar]
  18. Otter J. A., French G. L. 2011; Community-associated meticillin-resistant Staphylococcus aureus strains as a cause of healthcare-associated infection. J Hosp Infect 79:189193 [View Article][PubMed]
    [Google Scholar]
  19. Otter J. A., French G. L. 2012; Community-associated meticillin-resistant Staphylococcus aureus: the case for a genotypic definition. J Hosp Infect 81:143148 [View Article][PubMed]
    [Google Scholar]
  20. Otto M. 2010; Basis of virulence in community-associated methicillin-resistant Staphylococcus aureus . Annu Rev Microbiol 64:143162 [View Article][PubMed]
    [Google Scholar]
  21. Otto M. 2013; Community-associated MRSA: what makes them special?. Int J Med Microbiol 303:324330 [View Article][PubMed]
    [Google Scholar]
  22. Peleg A. Y., Monga D., Pillai S., Mylonakis E., Moellering R. C. Jr., Eliopoulos G. M. 2009; Reduced susceptibility to vancomycin influences pathogenicity in Staphylococcus aureus infection. J Infect Dis 199:532536 [View Article][PubMed]
    [Google Scholar]
  23. Ramarao N., Nielsen-Leroux C., Lereclus D. 2012; The insect Galleria mellonella as a powerful infection model to investigate bacterial pathogenesis. J Vis Exp 70:e4392
    [Google Scholar]
  24. Rasigade J. P., Trouillet-Assant S., Ferry T., Diep B. A., Sapin A., Lhoste Y., Ranfaing J., Badiou C., Benito Y. et al. 2013; PSMs of hypervirulent Staphylococcus aureus act as intracellular toxins that kill infected osteoblasts. PLoS One 8:e63176 [View Article][PubMed]
    [Google Scholar]
  25. Rautenberg M., Joo H. S., Otto M., Peschel A. 2011; Neutrophil responses to staphylococcal pathogens and commensals via the formyl peptide receptor 2 relates to phenol-soluble modulin release and virulence. FASEB J 25:12541263 [View Article][PubMed]
    [Google Scholar]
  26. Richards R. L., Haigh R. D., Pascoe B., Sheppard S. K., Price F., Jenkins D., Rajakumar K., Morrissey J. A. 2015; Persistent Staphylococcus aureus isolates from two independent cases of bacteremia display increased bacterial fitness and novel immune evasion phenotypes. Infect Immun 83:33113324 [View Article][PubMed]
    [Google Scholar]
  27. Tenover F. C., Goering R. V. 2009; Methicillin-resistant Staphylococcus aureus strain USA300: origin and epidemiology. J Antimicrob Chemother 64:441446 [View Article][PubMed]
    [Google Scholar]
  28. Thurlow L. R., Joshi G. S., Richardson A. R. 2012; Virulence strategies of the dominant USA300 lineage of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA). FEMS Immunol Med Microbiol 65:522 [View Article][PubMed]
    [Google Scholar]
  29. Tsompanidou E., Denham E. L., van Dijl J. M. 2013; Phenol-soluble modulins, hellhounds from the staphylococcal virulence-factor pandemonium. Trends Microbiol 21:313315 [View Article][PubMed]
    [Google Scholar]
  30. Wang R., Braughton K. R., Kretschmer D., Bach T. H., Queck S. Y., Li M., Kennedy A. D., Dorward D. W., Klebanoff S. J. et al. 2007; Identification of novel cytolytic peptides as key virulence determinants for community-associated MRSA. Nat Med 13:15101514 [View Article][PubMed]
    [Google Scholar]
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