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Volume 65, Issue 12

Comparative analysis of phenol-soluble modulin production and killing by community-associated and healthcare-associated meticillin-resistant strains Free

Abstract

Community-associated meticillin-resistant (CA-MRSA) have emerged globally and have been associated with more severe disease than healthcare-associated MRSA (HA-MRSA). The purpose of this study was to determine whether laboratory measures of virulence can distinguish dominant CA-MRSA clones from HA-MRSA clones. We compared the production of phenol-soluble modulins (PSMs) and ability to kill caterpillars for a range of CA- and HA-MRSA strains. Twenty-two HA-MRSA strains [ST22-IV (EMRSA-15), ST36-II (EMRSA-16) and ST239-III] and 26 CA-MRSA strains [ST1-IV (PVL+ USA400), ST1-IV (PVL–), ST8-IV (USA300), ST22-IV (PVL+), ST30-IV, ST59-IV and ST80-IV] were analysed. PSM production was measured using and compared using -tests and ANOVA. A (caterpillar) pathogenicity model was performed, and differences were compared using survival analysis and the log-rank test. There was no significant difference in overall PSM production between HA and CA strains (=0.090), but there was significant variation between clones (=0.003). caterpillar killing varied significantly by clone (<0.001), and overall killing was greater for HA compared with CA clones (=0.007). The increased acute virulence phenotype of CA-MRSA clones in humans is not associated with increased PSM production or increased killing in an caterpillar pathogenicity model.

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Keyword(s): community-associated , Galleria melonella , healthcare-associated , MRSA , PSM and virulence
© 2016 The Authors
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/content/journal/jmm/10.1099/jmm.0.000379
2016-12-16
2023-06-05
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Comparative analysis of phenol-soluble modulin production and Galleria mellonella killing by community-associated and healthcare-associated meticillin-resistant Staphylococcusaureus strains
J. Med. Microbiol. 65, 1429 (2016); https://doi.org/10.1099/jmm.0.000379
/content/journal/jmm/10.1099/jmm.0.000379
/content/journal/jmm/10.1099/jmm.0.000379
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