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Volume 65, Issue 12

Comparative evaluation of the AdvanSure Mycobacteria GenoBlot assay and the GenoType Mycobacterium CM/AS assay for the identification of non-tuberculous mycobacteria Free

Abstract

In this study, to assess the performance of the AdvanSure Mycobacteria GenoBlot assay (AdvanSure assay), we compared its performance with that of the GenoType Mycobacterium CM/AS assay (GenoType assay) for the identification of non-tuberculous mycobacteria (NTM). Twenty-four reference strains and 103 consecutive clinical NTM isolates were analysed. The accuracy rates for the 24 reference strains were 87.5 and 95.8 % for the AdvanSure and GenoType assays, respectively. For the 103 clinical isolates, a 91.3 % (94/103) concordance rate was observed between the two assays. The majority (7/9) of discrepancies were isolates identified as complex (MAC) by only the AdvanSure assay. All of these isolates except one were confirmed as MAC by sequence-based typing. The AdvanSure assay showed comparable performance to the GenoType assay and can be useful as a routine method for NTM identification in the clinical setting, especially where MAC is the main cause of NTM infection.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Identification , Line probe assay , Mycobacterium avium complex , Nontuberculous mycobacteria and Validation
© 2016 The Authors
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/content/journal/jmm/10.1099/jmm.0.000376
2016-12-16
2023-05-27
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Comparative evaluation of the AdvanSure Mycobacteria GenoBlot assay and the GenoType Mycobacterium CM/AS assay for the identification of non-tuberculous mycobacteria
J. Med. Microbiol. 65, 1422 (2016); https://doi.org/10.1099/jmm.0.000376
/content/journal/jmm/10.1099/jmm.0.000376
/content/journal/jmm/10.1099/jmm.0.000376
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