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Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 65, Issue 12

Comparative evaluation of the AdvanSure Mycobacteria GenoBlot assay and the GenoType Mycobacterium CM/AS assay for the identification of non-tuberculous mycobacteria No Access

Abstract

In this study, to assess the performance of the AdvanSure Mycobacteria GenoBlot assay (AdvanSure assay), we compared its performance with that of the GenoType Mycobacterium CM/AS assay (GenoType assay) for the identification of non-tuberculous mycobacteria (NTM). Twenty-four reference strains and 103 consecutive clinical NTM isolates were analysed. The accuracy rates for the 24 reference strains were 87.5 and 95.8 % for the AdvanSure and GenoType assays, respectively. For the 103 clinical isolates, a 91.3 % (94/103) concordance rate was observed between the two assays. The majority (7/9) of discrepancies were isolates identified as complex (MAC) by only the AdvanSure assay. All of these isolates except one were confirmed as MAC by sequence-based typing. The AdvanSure assay showed comparable performance to the GenoType assay and can be useful as a routine method for NTM identification in the clinical setting, especially where MAC is the main cause of NTM infection.

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Keyword(s): Identification , Line probe assay , Mycobacterium avium complex , Nontuberculous mycobacteria and Validation
© 2016 The Authors
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/content/journal/jmm/10.1099/jmm.0.000376
2016-12-16
2025-05-19
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References

  1. Adjemian J., Olivier K. N., Seitz A. E., Holland S. M., Prevots D. R. 2012; Prevalence of nontuberculous mycobacterial lung disease in U.S. Medicare beneficiaries. Am J Respir Crit Care Med 185:881–886 [View Article][PubMed]
     [Google Scholar]
  2. Bártfai Z., Somoskövi A., Ködmön C., Szabó N., Puskás E., Kosztolányi L., Faragó E., Mester J., Parsons L. M., Salfinger M. 2001; Molecular characterization of rifampin-resistant isolates of Mycobacterium tuberculosis from Hungary by DNA sequencing and the line probe assay. J Clin Microbiol 39:3736–3739 [View Article][PubMed]
     [Google Scholar]
  3. CLSI 2008; Interpretive Criteria for Identification of Bacteria and Fungi by DNA Target Sequencing; Approved Guideline. CLSI Document MM18-A. Wayne, PA: Clinical and Laboratory Standards Institute;
  4. De Beenhouwer H., Liang Z., De Rijk P., Van Eekeren C., Portaels F. 1995; Detection and identification of mycobacteria by DNA amplification and oligonucleotide-specific capture plate hybridization. J Clin Microbiol 33:2994–2998[PubMed]
     [Google Scholar]
  5. Della-Latta P. 2010; Digestion-decontamination procedures. In Clinical Microbiology Procedures Handbook pp. 7.2.1.1–7.2.1.7 Washington, DC: ASM Press;
     [Google Scholar]
  6. Franco-Alvarez de Luna F., Ruiz P., Gutiérrez J., Casal M. 2006; Evaluation of the GenoType Mycobacteria Direct assay for detection of Mycobacterium tuberculosis complex and four atypical mycobacterial species in clinical samples. J Clin Microbiol 44:3025–3027 [View Article][PubMed]
     [Google Scholar]
  7. García-Agudo L., Jesús I., Rodríguez-Iglesias M., García-Martos P. 2011; Evaluation of INNO-LiPA mycobacteria v2 assay for identification of rapidly growing mycobacteria. Braz J Microbiol 42:1220–1226 [View Article][PubMed]
     [Google Scholar]
  8. Giannini C., Giannelli F., Monti M., Careccia G., Marrocchi M. E., Laffi G., Gentilini P., Zignego A. L. 1999; Prevalence of mixed infection by different hepatitis C virus genotypes in patients with hepatitis C virus-related chronic liver disease. J Lab Clin Med 134:68–73 [View Article][PubMed]
     [Google Scholar]
  9. Gitti Z., Neonakis I., Fanti G., Kontos F., Maraki S., Tselentis Y. 2006; Use of the GenoType Mycobacterium CM and AS assays to analyze 76 nontuberculous mycobacterial isolates from Greece. J Clin Microbiol 44:2244–2246 [View Article][PubMed]
     [Google Scholar]
  10. Griffith D. E., Aksamit T., Brown-Elliott B. A., Catanzaro A., Daley C., Gordin F., Holland S. M., Horsburgh R., Huitt G. et al. 2007; An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. Am J Respir Crit Care Med 175:367–416 [View Article][PubMed]
     [Google Scholar]
  11. Griffith D. E. 2010; Nontuberculous mycobacterial lung disease. Curr Opin Infect Dis 23:185–190 [View Article][PubMed]
     [Google Scholar]
  12. Hain Lifescience 2011 GenoType Mycobacterium CM Package Insert Nehren, Germany: Hain Lifescience;
     [Google Scholar]
  13. Hofmann-Thiel S., Turaev L., Alnour T., Drath L., Müllerova M., Hoffmann H. 2011; Multi-centre evaluation of the speed-oligo Mycobacteria assay for differentiation of Mycobacterium spp. in clinical isolates. BMC Infect Dis 11:353 [View Article][PubMed]
     [Google Scholar]
  14. Huh H. J., Park K. S., Jang M. A., Kim J. Y., Kwon H. J., Ki C. S., Lee N. Y. 2014; Evaluation of performance of the Real-Q NTM-ID kit for rapid identification of eight nontuberculous mycobacterial species. J Clin Microbiol 52:4053–4055 [View Article][PubMed]
     [Google Scholar]
  15. Jang M. A., Koh W. J., Huh H. J., Kim S. Y., Jeon K., Ki C. S., Lee N. Y. 2014; Distribution of nontuberculous mycobacteria by multigene sequence-based typing and clinical significance of isolated strains. J Clin Microbiol 52:1207–1212 [View Article][PubMed]
     [Google Scholar]
  16. Kim B. J., Lee S. H., Lyu M. A., Kim S. J., Bai G. H., Chae G. T., Kim E. C., Cha C. Y., Kook Y. H. 1999; Identification of mycobacterial species by comparative sequence analysis of the RNA polymerase gene (rpoB). J Clin Microbiol 37:1714–1720[PubMed]
     [Google Scholar]
  17. Kim K. B., Park S. G., Park J. S., Lee W., Ha J. S., Ryoo N. H., Jeon D. S., Kim J. R. 2015; First case of pulmonary Mycobacterium parascrofulaceum infection in a patient with bronchiectasis in Korea. Ann Lab Med 35:379–381 [View Article][PubMed]
     [Google Scholar]
  18. Kodana M., Tarumoto N., Kawamura T., Saito T., Ohno H., Maesaki S., Ikebuchi K. 2016; Utility of the MALDI-TOF MS method to identify nontuberculous mycobacteria. J Infect Chemother 22:32–35 [View Article][PubMed]
     [Google Scholar]
  19. Lee A. S., Jelfs P., Sintchenko V., Gilbert G. L. 2009; Identification of non-tuberculous mycobacteria: utility of the GenoType Mycobacterium CM/AS assay compared with HPLC and 16S rRNA gene sequencing. J Med Microbiol 58:900–904 [View Article][PubMed]
     [Google Scholar]
  20. Legrand E., Sola C., Verdol B., Rastogi N. 2000; Genetic diversity of Mycobacterium avium recovered from AIDS patients in the Caribbean as studied by a consensus IS1245-RFLP method and pulsed-field gel electrophoresis. Res Microbiol 151:271–283 [View Article][PubMed]
     [Google Scholar]
  21. LG Life Sciences 2013 AdvanSure™ Mycobacteria GenoBlot Assay Package Insert Seoul, Korea: LG Life Sciences;
     [Google Scholar]
  22. Mäkinen J., Marjamäki M., Marttila H., Soini H. 2006; Evaluation of a novel strip test, GenoType Mycobacterium CM/AS, for species identification of mycobacterial cultures. Clin Microbiol Infect 12:481–483 [View Article][PubMed]
     [Google Scholar]
  23. Mareković I., Bošnjak Z., Jakopović M., Boras Z., Janković M., Popović-Grle S. 2016; Evaluation of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in identification of nontuberculous mycobacteria. Chemotherapy 61:167–170 [View Article][PubMed]
     [Google Scholar]
  24. Padilla E., González V., Manterola J. M., Pérez A., Quesada M. D., Gordillo S., Vilaplana C., Pallarés M. A., Molinos S. et al. 2004; Comparative evaluation of the new version of the INNO-LiPA Mycobacteria and GenoType Mycobacterium assays for identification of Mycobacterium species from MB/BacT liquid cultures artificially inoculated with mycobacterial strains. J Clin Microbiol 42:3083–3088 [View Article][PubMed]
     [Google Scholar]
  25. Park J. H., Shim T. S., Lee S. A., Lee H., Lee I. K., Kim K., Kook Y. H., Kim B. J. 2010; Molecular characterization of Mycobacterium intracellulare-related strains based on the sequence analysis of hsp65, internal transcribed spacer and 16S rRNA genes. J Med Microbiol 59:1037–1043 [View Article][PubMed]
     [Google Scholar]
  26. Pfyffer G. E. 2015; Mycobacterium: general characteristics, laboratory detection, and staining procedures. In Manual of Clinical Microbiology pp. 536–569 Washington, DC: ASM Press;
     [Google Scholar]
  27. Prevots D. R., Marras T. K. 2015; Epidemiology of human pulmonary infection with nontuberculous mycobacteria: a review. Clin Chest Med 36:13–34 [View Article][PubMed]
     [Google Scholar]
  28. Ramis I. B., Cnockaert M., Von Groll A., Mathys V., Simon A., Tortoli E., Palomino J. C., Almeida da Silva P. E., Vandamme P. et al. 2015; Evaluation of the Speed-Oligo Mycobacteria assay for the identification of nontuberculous mycobacteria. J Med Microbiol 64:283–287 [View Article][PubMed]
     [Google Scholar]
  29. Richter E., Rüsch-Gerdes S., Hillemann D. 2006; Evaluation of the GenoType Mycobacterium assay for identification of mycobacterial species from cultures. J Clin Microbiol 44:1769–1775 [View Article][PubMed]
     [Google Scholar]
  30. Rodríguez-Sánchez B., Ruiz-Serrano M. J., Ruiz A., Timke M., Kostrzewa M., Bouza E. 2016; Evaluation of MALDI biotyper Mycobacteria Library v3.0 for identification of nontuberculous mycobacteria. J Clin Microbiol 54:1144–1147 [View Article][PubMed]
     [Google Scholar]
  31. Russo C., Tortoli E., Menichella D. 2006; Evaluation of the new GenoType Mycobacterium assay for identification of mycobacterial species. J Clin Microbiol 44:334–339 [View Article][PubMed]
     [Google Scholar]
  32. Sarkola A., Mäkinen J., Marjamäki M., Marttila H. J., Viljanen M. K., Soini H. 2004; Prospective evaluation of the GenoType assay for routine identification of mycobacteria. Eur J Clin Microbiol Infect Dis 23:642–645 [View Article][PubMed]
     [Google Scholar]
  33. Schweickert B., Goldenberg O., Richter E., Göbel U. B., Petrich A., Buchholz P., Moter A. 2008; Occurrence and clinical relevance of Mycobacterium chimaera sp. nov., Germany. Emerg Infect Dis 14:1443–1446 [View Article][PubMed]
     [Google Scholar]
  34. Simons S., van Ingen J., Hsueh P. R., Van Hung N., Dekhuijzen P. N., Boeree M. J., van Soolingen D. 2011; Nontuberculous mycobacteria in respiratory tract infections, eastern Asia. Emerg Infect Dis 17:343–349 [View Article][PubMed]
     [Google Scholar]
  35. Singh A. K., Maurya A. K., Umrao J., Kant S., Kushwaha R. A., Nag V. L., Dhole T. N. 2013; Role of GenoType(®) Mycobacterium common mycobacteria/additional species assay for rapid differentiation between Mycobacterium tuberculosis complex and different species of non-tuberculous mycobacteria. J Lab Physicians 5:83–89 [View Article][PubMed]
     [Google Scholar]
  36. Somoskövi A., Ködmön C., Lantos A., Bártfai Z., Tamási L., Füzy J., Magyar P., Fuzy J. 2000; Comparison of recoveries of Mycobacterium tuberculosis using the automated BACTEC MGIT 960 system, the BACTEC 460 TB system, and Löwenstein-Jensen medium. J Clin Microbiol 38:2395–2397[PubMed]
     [Google Scholar]
  37. Telenti A., Marchesi F., Balz M., Bally F., Böttger E. C., Bodmer T. 1993; Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 31:175–178[PubMed]
     [Google Scholar]
  38. Tortoli E. 2003; Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s. Clin Microbiol Rev 16:319–354 [View Article][PubMed]
     [Google Scholar]
  39. van Ingen J., Bendien S. A., de Lange W. C., Hoefsloot W., Dekhuijzen P. N., Boeree M. J., van Soolingen D. 2009; Clinical relevance of non-tuberculous mycobacteria isolated in the Nijmegen-Arnhem region, The Netherlands. Thorax 64:502–506 [View Article][PubMed]
     [Google Scholar]
  40. van Ingen J. 2013; Diagnosis of nontuberculous mycobacterial infections. Semin Respir Crit Care Med 34:103–109 [View Article][PubMed]
     [Google Scholar]
  41. Weiss C. H., Glassroth J. 2012; Pulmonary disease caused by nontuberculous mycobacteria. Expert Rev Respir Med 6:597–612 [View Article][PubMed]
     [Google Scholar]
/content/journal/jmm/10.1099/jmm.0.000376
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Comparative evaluation of the AdvanSure Mycobacteria GenoBlot assay and the GenoType Mycobacterium CM/AS assay for the identification of non-tuberculous mycobacteria
J. Med. Microbiol. 65, 1422 (2016); https://doi.org/10.1099/jmm.0.000376
/content/journal/jmm/10.1099/jmm.0.000376
/content/journal/jmm/10.1099/jmm.0.000376
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