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Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 65, Issue 11

NDM-producing in a Chinese hospital, 2014–2015: identification of NDM-producing and acquisition of -carrying plasmid in a clinical isolate No Access

Abstract

New Delhi metallo-β-lactamase (NDM)-producing (NPE) shows prevalence in China. Little is known about the mechanisms related to the spread of NPE. Recently, a total of 51 non-duplicated NPE isolates were collected from a tertiary-care hospital in China and analysed for genetic relatedness by PFGE, antimicrobial susceptibility by Etest and sequence type by multilocus sequence typing. S1-PFGE and Southern blot analysis or PCR amplification were used for plasmid profiling. Between 2014 and 2015, 22 , 10 , 9 , 2 , 3 , 1 , 1 , 1 , 1 and 1 were identified as NPE. Results of PFGE and multilocus sequence typing showed that most strains were genetically unrelated. Among the 45 -carrying plasmids, there were 25 IncX3 plasmids with a size of about 30 to 50 kb, one 100 kb IncX3 plasmid, 11 IncA/C plasmids with a size range from 70 to 300 kb, six 90 to 120 kb IncB/O plasmids, one IncN plasmid with a size of 100 kb and one 140 kb IncFrep plasmid. An NDM-1-producing isolate of was identified, which had not been reported previously. An strain was found acquiring a -carrying IncFrep plasmid during infection. In conclusion, an NDM-1-producing isolate of was identified. An strain acquired a -carrying plasmid . IncX3 and IncA/C plasmids with various sizes might have emerged as the main platforms mediating the spread of the genes in China.

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Keyword(s): Citrobacter werkmanii , Enterobacteriaceae and NDM-1
© 2016 The Authors
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/content/journal/jmm/10.1099/jmm.0.000357
2016-11-16
2026-01-18

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/content/journal/jmm/10.1099/jmm.0.000357
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NDM-producing Enterobacteriaceae in a Chinese hospital, 2014–2015: identification of NDM-producing Citrobacterwerkmanii and acquisition of blaNDM-1-carrying plasmid in vivo in a clinical Escherichia coli isolate
J. Med. Microbiol. 65, 1253 (2016); https://doi.org/10.1099/jmm.0.000357
/content/journal/jmm/10.1099/jmm.0.000357
/content/journal/jmm/10.1099/jmm.0.000357
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