1887

Abstract

New Delhi metallo-β-lactamase (NDM)-producing (NPE) shows prevalence in China. Little is known about the mechanisms related to the spread of NPE. Recently, a total of 51 non-duplicated NPE isolates were collected from a tertiary-care hospital in China and analysed for genetic relatedness by PFGE, antimicrobial susceptibility by Etest and sequence type by multilocus sequence typing. S1-PFGE and Southern blot analysis or PCR amplification were used for plasmid profiling. Between 2014 and 2015, 22 , 10 , 9 , 2 , 3 , 1 , 1 , 1 , 1 and 1 were identified as NPE. Results of PFGE and multilocus sequence typing showed that most strains were genetically unrelated. Among the 45 -carrying plasmids, there were 25 IncX3 plasmids with a size of about 30 to 50 kb, one 100 kb IncX3 plasmid, 11 IncA/C plasmids with a size range from 70 to 300 kb, six 90 to 120 kb IncB/O plasmids, one IncN plasmid with a size of 100 kb and one 140 kb IncFrep plasmid. An NDM-1-producing isolate of was identified, which had not been reported previously. An strain was found acquiring a -carrying IncFrep plasmid during infection. In conclusion, an NDM-1-producing isolate of was identified. An strain acquired a -carrying plasmid . IncX3 and IncA/C plasmids with various sizes might have emerged as the main platforms mediating the spread of the genes in China.

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2016-11-16
2020-01-29
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