1887

Abstract

This study aimed to determine the occurrence of , and spp., in relation to clinical signs of respiratory disease. Tracheobronchial lavage samples were collected from 96 (healthy and unhealthy) cattle in the State of São Paulo, Brazil. spp. (12.5 %) and (15.50 %) were the most prevalent species. sp., sp., , , and were also isolated. (70.83 %), (2.94 %) and (38.23 %) were identified using conventional PCR. Submassive sound on acoustic percussion of the thorax was associated with the absence of =0.025). Whistling (=0.076) and coarse crackle (=0.046) were associated with the absence of . Clear sound on acoustic percussion of the thorax was associated with the absence of (=0.007). Coughing was associated with the presence of [=0.035; confidence interval (CI), 1.12–26.89], but its absence was associated with mucopurulent (=0.0215; CI, 1.55–34.5) and mucoid nasal discharge (=0.068; CI, 19–28.5), submassive sound (=0.031; CI, 1.23–75.5), fine crackle (=0.058; CI, 1.23–20.1) and coarse crackle (=0.046; CI, 2.38–70.8). The high prevalence of and spp. in unhealthy calves increases the importance of these micro-organisms in the pathogenesis of respiratory diseases. This study increases the information about the role of in respiratory diseases. Differences in some species in relation to clinical signs can be applied as a presumptive diagnosis.

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2016-10-18
2021-08-01
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References

  1. Aebi M., van den Borne B. H., Raemy A., Steiner A., Pilo P., Bodmer M. 2015; Mycoplasma bovis infections in Swiss dairy cattle: a clinical investigation. Acta Vet Scand 57:1–11 [View Article][PubMed]
    [Google Scholar]
  2. Akan M., Babacan O., Torun E., Mustak H. K., Oncel T. 2014; Diagnosis of Mycoplasma bovis infection in cattle by ELISA and PCR. KAFKAS Univ Vet Fak Derg 20:249–252
    [Google Scholar]
  3. Alemayehu G., Leta S., Hailu B. 2015; Sero-prevalence of contagious bovine pleuropneumonia (CBPP) in bulls originated from Borena pastoral area of Southern Ethiopia. Trop Anim Health Prod 47:983–987 [View Article][PubMed]
    [Google Scholar]
  4. Alhaji N. B., Babalobi O. O. 2015; Sero-positivity and associated risk factors for contagious bovine pleuropneumonia under two cattle production systems in North Central Nigeria. Trop Anim Health Prod 48:311–320 [View Article][PubMed]
    [Google Scholar]
  5. Angen O., Thomsen J., Larsen L. E., Larsen J., Kokotovic B., Heegaard P. M. H., Enemark J. M. D. 2009; Respiratory disease in calves: microbiological investigations on trans-tracheally aspirated bronchoalveolar fluid and acute phase protein response. Vet Microbio 137:165–171 [CrossRef]
    [Google Scholar]
  6. Barros M. S. R. M., Castro R. S., Tabosa J. H. C., Brito M. F., Amaral B. 1994; Colheita do fluído brônquico-alveolar de bezerros através da traqueocêntese transcutânea. Arq Bras Med Vet Zootec 46:41–49
    [Google Scholar]
  7. Benesi F. J., Bertagnon H. G., Wachholz L., Leal M. L. R., Fernandes W. R., Benites N. R., Melville P. A. 2013; Microbiota bacteriana e citologia da região traqueobrônquica de bezerros no período neonatal. Pesqui Vet Bras 33:700–704 [View Article]
    [Google Scholar]
  8. Castillo-Alcala F., Bateman K. G., Cai H. Y., Schott C. R., Parker L., Clark M. E., McRaild P., McDowall R. M., Foster R. A. et al. 2012; Prevalence and genotype of Mycoplasma bovis in beef cattle after arrival at a feedlot. Am J Vet Res 73:1932–1943 [View Article][PubMed]
    [Google Scholar]
  9. Chávez González Y. R., Ros Bascuñana C., Bölske G., Mattsson J. G., Fernández Molina C., Johansson K. E. 1995; In vitro amplification of the 16S rRNA genes from Mycoplasma bovis and Mycoplasma agalactiae by PCR. Vet Microbiol 47:183–190 [View Article][PubMed]
    [Google Scholar]
  10. de Jong A., Thomas V., Simjee S., Moyaert H., El Garch F., Maher K., Morrissey I., Butty P., Klein U. et al. 2014; Antimicrobial susceptibility monitoring of respiratory tract pathogens isolated from diseased cattle and pigs across Europe: the vetpath study. Vet Microbiol 172:202–215 [View Article][PubMed]
    [Google Scholar]
  11. Dedieu L., Mady V., Lefevre P. C. 1994; Development of a selective polymerase chain reaction assay for the detection of Mycoplasma mycoides subsp. mycoides S.C. (contagious bovine pleuropneumonia agent). Vet Microbiol 42:327–339 [View Article][PubMed]
    [Google Scholar]
  12. Francoz D., Buczinski S., Bélanger A. M., Forté G., Labrecque O., Tremblay D., Wellemans V., Dubuc J. 2015; Respiratory pathogens in Québec dairy calves and their relationship with clinical status, lung consolidation, and average daily gain. J Vet Intern Med 29:381–387 [View Article][PubMed]
    [Google Scholar]
  13. Gabinaitiene A., Siugzdaite J., Zilinskas H., Siugzda R. 2011; Mycoplasma bovis and bacterial pathogens in the bovine respiratory tract. Vet Med 56:28–34
    [Google Scholar]
  14. Gonçalves R. C. 1987 Estudo Da Flora Traqueobrônquica Em Bezerros Clinicamente Sadios E Portadores De Pneumonia, Na Região De Botucatu, SP Faculdade de Medicina Veterinária e Zootecnia: Universidade Estadual Paulista, Brazil (in Portuguese);
    [Google Scholar]
  15. Gonçalves R. C. 2014 In Semiologia Do Sistema Respiratório, 3rd edn. pp. 313–331 Edited by Feitosa F. L. F. São Paulo: Roca (in Portuguese);
    [Google Scholar]
  16. Griffin D. 2010; Bovine pasteurellosis and other bacterial infections of the respiratory tract. Vet Clin North Am Food Anim Pract 26:57–71 [View Article][PubMed]
    [Google Scholar]
  17. Griffin D., Chengappa M. M., Kuszak J., McVey D. S. 2010; Bacterial pathogens of the bovine respiratory disease complex. Vet Clin North Am Food Anim Pract 26:381–394 [View Article][PubMed]
    [Google Scholar]
  18. Holman D. B., McAllister T. A., Topp E., Wright A. D., Alexander T. W. 2015; The nasopharyngeal microbiota of feedlot cattle that develop bovine respiratory disease. Vet Microbiol 180:90–95 [View Article][PubMed]
    [Google Scholar]
  19. Horwood P. F., Schibrowski M. I., Fowler E. V., Gibson J. S., Barnes T. S., Mahony T. J. 2014; Is Mycoplasma bovis a missing component of the bovine respiratory disease complex in Australia?. Aust Vet J 92:185–191 [View Article][PubMed]
    [Google Scholar]
  20. Marques L. M., Buzinhani M., Yamaguti M., Oliveira R. C., Ferreira J. B., Mettifogo E., Timenetsky J. 2007a; Use of a polymerase chain reaction for detection of Mycoplasma dispar in the nasal mucus of calves. J Vet Diagn Invest 19:103–106 [View Article]
    [Google Scholar]
  21. Marques L. M., Buzinhani M., Oliveira R. C., Yamaguti M., Ferreira J. B., Neto R. L., Timenetsky J., Mettifogo J. T. 2007b; Prevalence of mycoplasmas in the respiratory tracts of calves in Brazil. Vet Rec 161:699–700 [View Article]
    [Google Scholar]
  22. Miles D. G. 2009; Overview of the North American beef cattle industry and the incidence of bovine respiratory disease (BRD). Anim Health Res Rev 10:101–103 [View Article][PubMed]
    [Google Scholar]
  23. Moore S. J., O'Dea M. A., Perkins N., O'Hara A. J. 2015; Estimation of nasal shedding and seroprevalence of organisms known to be associated with bovine respiratory disease in Australian live export cattle. J Vet Diagn Invest 27:6–17 [View Article][PubMed]
    [Google Scholar]
  24. Nanduri B., Rai A., Epperson W. 2015; Application of functional genomics for bovine respiratory disease diagnostics. Bioinform Biol Insights 9:13–23 [CrossRef]
    [Google Scholar]
  25. OIE 2014; Contagious bovine pleuropneumonia. In Terr Man Online pp 1–16 Organizaçao Internacional de Epizootias;
    [Google Scholar]
  26. Pardon B., De Bleecker K., Dewulf J., Callens J., Boyen F., Catry B., Deprez P. 2011; Prevalence of respiratory pathogens in diseased, non-vaccinated, routinely medicated veal calves. Vet Rec 169:278 [View Article][PubMed]
    [Google Scholar]
  27. Radostits O. M., Gay C. C., Blood D. C., Hinchcliff K. W. 2007 Veterinary Medice: A Textbook of the Diseases of Cattle, Horses, Sheep, Pigs and Goats, 10th edn. Philadelphia: Elsevier;
    [Google Scholar]
  28. Rosenberger G. 1993 Exame Clínico Dos Bovinos, 3rd edn. Rio de Janeiro: Guanabara Koogan S.A;
    [Google Scholar]
  29. Séry A., Sidibé C. A., Cissé O., Diallo M., Koné M., Waret-Szkuta A., Roger F., Thiaucourt F., Niang M. 2014; Seroprevalence of contagious bovine pleuropneumonia (CBPP) in Mali. Trop Anim Health Prod 47:395–402 [View Article][PubMed]
    [Google Scholar]
  30. Šiugždaitė J., Gabinaitienė A., Šiugžda R. 2015; Mycoplasma dispar prevalence in the upper respiratory tract of cattle and the antimicrobial susceptibility of the isolates. Chem Technol 65:doi: 10.5755/j01.ct.65.1.8716 [CrossRef]
    [Google Scholar]
  31. Soehnlen M. K., Aydin A., Murthy K. S., Lengerich E. J., Hattel A. L., Houser B. A., Fenton G. D., Lysczek H. R., Burns C. M. et al. 2012; Epidemiology of Mycoplasma bovis in Pennsylvania veal calves. J Dairy Sci 95:247–254 [View Article][PubMed]
    [Google Scholar]
  32. Srivastava N. C., Thiaucourt F., Singh V. P., Sunder J., Singh V. P. 2000; Isolation of Mycoplasma mycoides small colony type from contagious caprine pleuropneumonia in India. Vet Rec 147:520–521 [View Article][PubMed]
    [Google Scholar]
  33. Tully J. G. 1995; Culture medium formulation for primary isolation and maintenance of mollicutes. In Molecular and Diagnostic Procedures in Mycoplasmology pp 33–40 . Edited by Tully J. G., Razin S. San Diego: Academic Press Inc; [CrossRef]
    [Google Scholar]
  34. USDA 2013; Feedlot 2001. Part IV: Health and Health Management on U.S. Feedlots with a capacity of 1000 or more head. In APHIS Fort Collins: National Animal Health Monitoring System;
    [Google Scholar]
  35. van Kuppeveld F. J., van der Logt J. T., Angulo A. F., van Zoest M. J., Quint W. G., Niesters H. G., Galama J. M., Melchers W. J. 1992; Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification. Appl Environ Microbiol 58:2606–2615[PubMed]
    [Google Scholar]
  36. Viana L., Gonçalves R. C., Oliveira Filho J. P., Paes A. C., Amorim R. M. 2007; Ocorrência de Mannheimia haemolytica e de Pasteurella multocida em ovinos sadios e com enfermidade respiratória. Arquivo Brasileiro De Medicina Veterinária E Zootecnia 59:1579–1582 [View Article]
    [Google Scholar]
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