1887

Abstract

Infections and outbreaks caused by multidrug-resistant (MDR-AB) are prevalent and have been reported worldwide over the past 20 or more years. Class 1 integron in MDR-AB plays an important role in the spread of antibiotic resistance in clinical settings. This study has been conducted to evaluate the detection of metallo-β-lactamase, characterization of class 1 integron and determination of clonal relatedness among hospital isolates. Sixty-five clinical isolates of MDR-AB were recovered from two Iranian hospital’s intensive care units from February to August 2013. Integrase () and genes were detected in 70.8 % (=46/65) and 9.23 % (=6/65) of the isolates using PCR assay, respectively. No other metallo-β-lactamase genes ( , and ) were detected. PCR sequencing of integron gene cassette revealed the following arrays: - - (as a novel array was designated In), and . Analysis of gene revealed a new allele designated as . Gene transfer experiment by conjugation showed the 36 kb conjugative plasmid harbouring In The clonal assessment by repetitive extragenic palindromic PCR demonstrated a high-degree relatedness among the strains, but strains harbouring In displayed a different repetitive extragenic palindromic PCR profile. In this study, we found that a novel class 1 integron (In) that encoded a new allele resided on a transferable plasmid in non-clonal strains of MDR-AB.

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2016-09-01
2020-01-18
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