1887

Abstract

Human papillomavirus (HPV) and are pathogens with oncogenic potential associated with persistent infections. Epidemiological data on infection status, /HPV co-infection and the relationship between HPV genotypes in Italian women are only preliminary. The aim of the present study was to characterize the relationship between HPV genotypes and in an extending cohort of asymptomatic immunocompetent women from an area of north-east Italy. A retrospective study was conducted using Luminex technology on cervical swabs from asymptomatic immunocompetent women, comprising 921 attending the prevention centre for the Cervical Cancer Program and 6214 who had been referred to the Sexually Transmitted Infections Center, with clinical indications of HPV and infections. A quantitative real-time PCR was performed to assess chronic infection by heat-shock protein 60 () gene expression. The overall prevalence of the investigated pathogens was 39 % (359/921) for HPV and 4 % (251/6214) for . The gene was detected in 57 % of the women infected with . HPV co-infection was present in 58 % of -infected women. A high prevalence of co-infection was found in women with chronic infection (68 %,  = 0.0002), especially in women ≤ 25 years (72 %) where HPV multiple infections were found in 78 % ( = 0.022). HPV genotype distribution showed that uncommon low-risk genotypes were associated with . These results indicate a high frequency of co-detection of multiple HPV genotypes in chronically infected young women and suggest that the expression of the gene may favour HPV infection.

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2016-05-01
2020-11-30
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References

  1. Agarossi A., Ferrazzi E., Parazzini F., Perno C. F., Ghisoni L.. 2009; Prevalence and type distribution of high-risk human papillomavirus infection in women undergoing voluntary cervical cancer screening in Italy. J Med Virol81:529–535 [CrossRef][PubMed]
    [Google Scholar]
  2. Ault K. A.. 2006; Epidemiology and natural history of human papillomavirus infections in the female genital tract. Infect Dis Obstet Gynecol2006:(Suppl.)40470 [CrossRef][PubMed]
    [Google Scholar]
  3. Bellaminutti S., Seraceni S., De Seta F., Gheit T., Tommasino M., Comar M.. 2014; HPV and Chlamydia trachomatis co-detection in young asymptomatic women from high incidence area for cervical cancer. J Med Virol86:1920–1925 [CrossRef][PubMed]
    [Google Scholar]
  4. Bhatla N., Puri K., Joseph E., Kriplani A., Iyer V. K., Sreenivas V.. 2013; Association of Chlamydia trachomatis infection with human papillomavirus (HPV) & cervical intraepithelial neoplasia – a pilot study. Indian J Med Res137:533–539[PubMed]
    [Google Scholar]
  5. Bianchi S., Frati E. R., Panatto D., Martinelli M., Amicizia D., Zotti C. M., Martinese M., Bonanni P., Boccalini S., other authors. 2013; Detection and genotyping of human papillomavirus in urine samples from unvaccinated male and female adolescents in Italy. PLoS One8:e79719 [CrossRef][PubMed]
    [Google Scholar]
  6. Bosch F. X., Broker T. R., Forman D., Moscicki A. B., Gillison M. L., Doorbar J., Stern P. L., Stanley M., Arbyn M., other authors. 2013; Comprehensive control of human papillomavirus infections and related diseases. Vaccine31:(Suppl. 7)H1–H31 [CrossRef][PubMed]
    [Google Scholar]
  7. Brankatschk R., Bodenhausen N., Zeyer J., Bürgmann H.. 2012; Simple absolute quantification method correcting for quantitative PCR efficiency variations for microbial community samples. Appl Environ Microbiol78:4481–4489 [CrossRef][PubMed]
    [Google Scholar]
  8. Cappello F., Conway de Macario E., Di Felice V., Zummo G., Macario A. J.. 2009; Chlamydia trachomatis infection and anti-Hsp60 immunity: the two sides of the coin. PLoS Pathog5:e1000552 [CrossRef][PubMed]
    [Google Scholar]
  9. Carozzi F., De Marco L., Gillio-Tos A., Del Mistro A., Girlando S., Baboci L., Trevisan M., Burroni E., Grasso S., other authors. 2014; Age and geographic variability of human papillomavirus high-risk genotype distribution in a large unvaccinated population and of vaccination impact on HPV prevalence. J Clin Virol60:257–263 [CrossRef][PubMed]
    [Google Scholar]
  10. Castle P. E., Escoffery C., Schachter J., Rattray C., Schiffman M., Moncada J., Sugai K., Brown C., Cranston B., other authors. 2003; Chlamydia trachomatis, herpes simplex virus 2, and human T-cell lymphotrophic virus type 1 are not associated with grade of cervical neoplasia in Jamaican colposcopy patients. Sex Transm Dis30:575–580 [CrossRef][PubMed]
    [Google Scholar]
  11. Comar M., Iannacone M. R., Casalicchio G., McKay-Chopin S., Tommasino M., Gheit T.. 2012; Comparison of hybrid capture II, linear array, and a bead-based multiplex genotyping assay for detection of human papillomavirus in women with negative pap test results and atypical squamous cells of undetermined significance. J Clin Microbiol50:4041–4046 [CrossRef][PubMed]
    [Google Scholar]
  12. Comparetto C., Borruto F.. 2015; Cervical cancer screening: a never-ending developing program. World J Clin Cases3:614–624[PubMed]
    [Google Scholar]
  13. Contini C., Seraceni S.. 2012; Chlamydial disease: a crossroad between chronic infection and development of cancer. [CrossRef] In Bacteria and Cancer pp79–116 Edited by Khan A. A.. Switzerland: Springer Science+Business Media BV;
    [Google Scholar]
  14. Contini C., Seraceni S., Cultrera R., Incorvaia C., Sebastiani A., Picot S.. 2005; Evaluation of a Real-time PCR-based assay using the lightcycler system for detection of Toxoplasma gondii bradyzoite genes in blood specimens from patients with toxoplasmic retinochoroiditis. Int J Parasitol35:275–283 [CrossRef][PubMed]
    [Google Scholar]
  15. Contini C., Grilli A., Badia L., Guardigni V., Govoni M., Seraceni S.. 2011; Detection of Chlamydophila pneumoniae in patients with arthritis: significance and diagnostic value. Rheumatol Int31:1307–1313 [CrossRef][PubMed]
    [Google Scholar]
  16. de Paula F. D., Fernandes A. P., Carmo B. B., Vieira D. C., Dutra M. S., Santos C. G., Souza M. C., Andrade T. C., Vago A. R., Fernandes P. A.. 2007; Molecular detection of Chlamydia trachomatis and HPV infections in cervical samples with normal and abnormal cytopathological findings. Diagn Cytopathol35:198–202 [CrossRef][PubMed]
    [Google Scholar]
  17. de Sanjosé S., Diaz M., Castellsagué X., Clifford G., Bruni L., Muñoz N., Bosch F. X.. 2007; Worldwide prevalence and genotype distribution of cervical human papillomavirus DNA in women with normal cytology: a meta-analysis. Lancet Infect Dis7:453–459 [CrossRef][PubMed]
    [Google Scholar]
  18. Dean D., Kandel R. P., Adhikari H. K., Hessel T.. 2008; Multiple Chlamydiaceae species in trachoma: implications for disease pathogenesis and control. PLoS Med5:e14 [CrossRef][PubMed]
    [Google Scholar]
  19. Deluca G. D., Basiletti J., Schelover E., Vásquez N. D., Alonso J. M., Marín H. M., Lucero R. H., Picconi M. A.. 2011; Chlamydia trachomatis as a probable cofactor in human papillomavirus infection in aboriginal women from northeastern Argentina. Braz J Infect Dis15:567–572 [CrossRef][PubMed]
    [Google Scholar]
  20. Eggleston E., Rogers S. M., Turner C. F., Miller W. C., Roman A. M., Hobbs M. M., Erbelding E., Tan S., Villarroel M. A., Ganapathi L.. 2011; Chlamydia trachomatis infection among 15- to 35-year-olds in Baltimore, MD. Sex Transm Dis38:743–749[PubMed]
    [Google Scholar]
  21. Giorgi Rossi P., Chini F., Bisanzi S., Burroni E., Carillo G., Lattanzi A., Angeloni C., Scalisi A., Macis R., other authors. 2011; Distribution of high and low risk HPV types by cytological status: a population based study from Italy. Infect Agent Cancer6:2–8 [CrossRef][PubMed]
    [Google Scholar]
  22. IARC Working Group on the Evaluation of Carcinogenic Risks to Humans 2012; Biological agents: a review of human carcinogens. In IARC Monographs on the Evaluation of Carcinogenic Risks to Humansvol. 100B1–441 Lyon, France: International Agency for Research on Cancer;
    [Google Scholar]
  23. Idahl A., Lundin E., Elgh F., Jurstrand M., Møller J. K., Marklund I., Lindgren P., Ottander U.. 2010; Chlamydia trachomatis, Mycoplasma, genitalium, Neisseria gonorrhoeae, human papillomavirus, and polyomavirus are not detectable in human tissue with epithelial ovarian cancer, borderline tumor, or benign conditions. Am J Obstet Gynecol202:e1–e6[PubMed][CrossRef]
    [Google Scholar]
  24. Jensen K. E., Thomsen L. T., Schmiedel S., Frederiksen K., Norrild B., van den Brule A., Iftner T., Kjær S. K.. 2014; Chlamydia trachomatis and risk of cervical intraepithelial neoplasia grade 3 or worse in women with persistent human papillomavirus infection: a cohort study. Sex Transm Infect90:550–555 [CrossRef][PubMed]
    [Google Scholar]
  25. Lehtinen M., Ault K. A., Lyytikainen E., Dillner J., Garland S. M., Ferris D. G., Koutsky L. A., Sings H. L., Lu S., other authors. 2011; Chlamydia trachomatis infection and risk of cervical intraepithelial neoplasia. Sex Transm Infect87:372–376 [CrossRef][PubMed]
    [Google Scholar]
  26. Ljubin-Sternak S., Meštrović T.. 2014; Chlamydia trachomatis and genital mycoplasmas: pathogens with an impact on human reproductive health. J Pathogens2014:183167 [CrossRef][PubMed]
    [Google Scholar]
  27. Luostarinen T., Namujju P. B., Merikukka M., Dillner J., Hakulinen T., Koskela P., Paavonen J., Surcel H. M., Lehtinen M.. 2013; Order of HPV/Chlamydia infections and cervical high-grade precancer risk: a case–cohort study. Int J Cancer133:1756–1759 [CrossRef][PubMed]
    [Google Scholar]
  28. Malinverni R.. 1996; The role of cytokines in chlamydial infections. Curr Opin Infect Dis9:150–155 [CrossRef]
    [Google Scholar]
  29. Naucler P., Chen H. C., Persson K., You S. L., Hsieh C. Y., Sun C. A., Dillner J., Chen C. J.. 2007; Seroprevalence of human papillomaviruses and Chlamydia trachomatis and cervical cancer risk: nested case–control study. J Gen Virol88:814–822 [CrossRef][PubMed]
    [Google Scholar]
  30. Oakeshott P., Aghaizu A., Reid F., Howell-Jones R., Hay P. E., Sadiq S. T., Lacey C. J., Beddows S., Soldan K.. 2012; Frequency and risk factors for prevalent, incident, and persistent genital carcinogenic human papillomavirus infection in sexually active women: community based cohort study. BMJ344:e4168 [CrossRef][PubMed]
    [Google Scholar]
  31. Paavonen J.. 2012; Chlamydia trachomatis infections of the female genital tract: state of the art. Ann Med44:18–28 [CrossRef][PubMed]
    [Google Scholar]
  32. Panatto D., Amicizia D., Bianchi S., Frati E. R., Zotti C. M., Lai P. L., Domnich A., Colzani D., Gasparini R., Tanzi E.. 2015; Chlamydia trachomatis prevalence and chlamydial/HPV co-infection among HPV-unvaccinated young Italian females with normal cytology. Hum Vaccin Immunother11:270–276 [CrossRef][PubMed]
    [Google Scholar]
  33. Piana A., Sotgiu G., Cocuzza C., Musumeci R., Marras V., Pischedda S., Deidda S., Muresu E., Castiglia P.. 2013; High HPV-51 prevalence in invasive cervical cancers: results of a pre-immunization survey in North Sardinia, Italy. PLoS One8:e63395 [CrossRef][PubMed]
    [Google Scholar]
  34. Quint K. D., de Koning M. N., Geraets D. T., Quint W. G., Pirog E. C.. 2009; Comprehensive analysis of human papillomavirus and Chlamydia trachomatis in in-situ and invasive cervical adenocarcinoma. Gynecol Oncol114:390–394 [CrossRef][PubMed]
    [Google Scholar]
  35. Seraceni S., De Seta F., Colli C., Del Savio R., Pesel G., Zanin V., D'Agaro P., Contini C., Comar M.. 2014; High prevalence of HPV multiple genotypes in women with persistent Chlamydia trachomatis infection. Infect Agent Cancer9:30 [CrossRef][PubMed]
    [Google Scholar]
  36. Shew M. L., Ermel A. C., Weaver B. A., Tong Y., Tu W., Kester L. M., Denski C., Fortenberry J. D., Brown D. R.. 2013; Association of Chlamydia trachomatis infection with redetection of human papillomavirus after apparent clearance. J Infect Dis208:1416–1421 [CrossRef][PubMed]
    [Google Scholar]
  37. Silins I., Ryd W., Strand A., Wadell G., Törnberg S., Hansson B. G., Wang X., Arnheim L., Dahl V., other authors. 2005; Chlamydia trachomatis infection and persistence of human papillomavirus. Int J Cancer116:110–115 [CrossRef][PubMed]
    [Google Scholar]
  38. Silva J., Cerqueira F., Ribeiro J., Sousa H., Osório T., Medeiros R.. 2013; Is Chlamydia trachomatis related to human papillomavirus infection in young women of southern European population? A self-sampling study. Arch Gynecol Obstet288:627–633 [CrossRef][PubMed]
    [Google Scholar]
  39. Silva J., Cerqueira F., Medeiros R.. 2014; Chlamydia trachomatis infection: implications for HPV status and cervical cancer. Arch Gynecol Obstet289:715–723 [CrossRef][PubMed]
    [Google Scholar]
  40. Simonetti A. C., Humberto de Lima Melo J., Eleutério de Souza P. R., Bruneska D., Luiz de Lima Filho J.. 2009; Immunological's host profile for HPV and Chlamydia trachomatis, a cervical cancer cofactor. Microbes Infect11:435–442 [CrossRef][PubMed]
    [Google Scholar]
  41. Soper D. E.. 2010; Pelvic inflammatory disease. Obstet Gynecol116:419–428 [CrossRef][PubMed]
    [Google Scholar]
  42. Tavares M. C., de Macêdo J. L., de Lima Júnior S. F., de Andrade Heráclio S., Amorim M. M., de Mascena Diniz Maia M., de Souza P. R.. 2014; Chlamydia trachomatis infection and human papillomavirus in women with cervical neoplasia in Pernambuco-Brazil. Mol Biol Rep41:865–874 [CrossRef][PubMed]
    [Google Scholar]
  43. Tommasino M.. 2014; The human papillomavirus family and its role in carcinogenesis. Semin Cancer Biol26:13–21 [CrossRef] (Review).[PubMed]
    [Google Scholar]
  44. Vidhani S., Mehta S., Bhalla P., Bhalla R., Sharma V. K., Batra S.. 2005; Seroprevalence of Chalmydia trachomatis infection amongst patients with pelvic inflammatory diseases and infertility. J Commun Dis37:233–238[PubMed]
    [Google Scholar]
  45. Vriend H. J., Bogaards J. A., van Bergen J. E., Brink A. A., van den Broek I. V., Hoebe C. J., King A. J., van der Sande M. A., Wolffs P. F., de Melker H. E., Medical Microbiological Laboratories and the CSI group. 2015; Incidence and persistence of carcinogenic genital human papillomavirus infections in young women with or without Chlamydia trachomatis co-infection. Cancer Med4:1589–1598 [CrossRef][PubMed]
    [Google Scholar]
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