1887

Abstract

is an important opportunistic bacterial pathogen responsible for serious infections in hospitalized patients. From a total of 78 consecutive non-repetitive spp. isolates from patients with blood infections, 61 were carbapenem resistant, which were positive for (96.7 %), (77 %), (8.1 %) and genes (32.8 %) by multiplex PCR. The isolates were identified as ( = 59) and ( = 2). Also, we found a case of , causing bacteraemia, that possessed the IMP gene. High levels of resistance were observed to fluoroquinolones, aminoglycosides, tigecycline and to the beta-lactam antibiotics, including piperacillin/tazobactam and ampicillin/sulbactam. IS was present in 96.7 % of all complex (Acb) isolates. Also, 33 (54.1 %) and 23 (37.7 %) isolates harboured IS upstream of and genes, respectively, though this was not observed in isolates. No relationship was observed between the presence of IS upstream of oxacillinase genes and the level of carbapenem resistance in all Acb isolates. Only two genes encoding metallo-beta-lactamase (VIM, SPM) were detected in all Acb isolates. This suggests that carbapenem resistance in blood-isolate Acb is mostly due to the presence of acquired carbapenemases. This is the first report from Iran on the identification of isolates that possess multiple oxacillinase genes and lack upstream IS

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2016-03-01
2019-10-15
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References

  1. Bergogne-Bérézin E., Towner K. J.. ( 1996;). Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin Microbiol Rev 9: 148–165 [PubMed].
    [Google Scholar]
  2. Bonnin R. A., Docobo-Pérez F., Poirel L., Villegas M. V., Nordmann P.. ( 2014;). Emergence of OXA-72-producing Acinetobacter pittii clinical isolates. Int J Antimicrob Agents 43: 195–196 [CrossRef] [PubMed].
    [Google Scholar]
  3. Boo T. W., Walsh F., Crowley B.. ( 2006;). First report of OXA-23 carbapenemase in clinical isolates of Acinetobacter species in the Irish Republic. J Antimicrob Chemother 58: 1101–1102 [CrossRef] [PubMed].
    [Google Scholar]
  4. Boo T. W., Walsh F., Crowley B.. ( 2009;). Molecular characterization of carbapenem-resistant Acinetobacter species in an Irish university hospital: predominance of Acinetobacter genomic species 3. J Med Microbiol 58: 209–216 [CrossRef] [PubMed].
    [Google Scholar]
  5. CLSI ( 2014;). Performance Standards for Antimicrobial Susceptibility Testing Twenty-Fourth Informational Supplement Wayne, PA: Clinical and Laboratory Standards Institute;.
    [Google Scholar]
  6. Ellington M. J., Kistler J., Livermore D. M., Woodford N.. ( 2007;). Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases. J Antimicrob Chemother 59: 321–322 [CrossRef] [PubMed].
    [Google Scholar]
  7. Evans B. A., Hamouda A., Towner K. J., Amyes S. G.. ( 2010;). Novel genetic context of multiple bla OXA-58 genes in Acinetobacter genospecies 3. J Antimicrob Chemother 65: 1586–1588 [CrossRef] [PubMed].
    [Google Scholar]
  8. Gerner-Smidt P., Tjernberg I., Ursing J.. ( 1991;). Reliability of phenotypic tests for identification of Acinetobacter species. J Clin Microbiol 29: 277–282 [PubMed].
    [Google Scholar]
  9. Gundi V. A., Dijkshoorn L., Burignat S., Raoult D., La Scola B.. ( 2009;). Validation of partial rpoB gene sequence analysis for the identification of clinically important and emerging Acinetobacter species. Microbiology 155: 2333–2341 [CrossRef] [PubMed].
    [Google Scholar]
  10. Higgins P. G., Lehmann M., Wisplinghoff H., Seifert H.. ( 2010;). gyrB multiplex PCR to differentiate between Acinetobacter calcoaceticus and Acinetobacter genomic species 3. J Clin Microbiol 48: 4592–4594 [CrossRef] [PubMed].
    [Google Scholar]
  11. Japoni S., Farshad S., Abdi Ali A., Japoni A.. ( 2011;). Antibacterial susceptibility patterns and cross-resistance of acinetobacter, isolated from hospitalized patients, southern iran. Iran Red Crescent Med J 13: 832–836 [PubMed].
    [Google Scholar]
  12. Kim D. H., Choi J. Y., Jung S. I., Thamlikitkul V., Song J. H., Ko K. S.. ( 2012;). AbaR4-type resistance island including the blaOXA-23 gene in Acinetobacter nosocomialis isolates. Antimicrob Agents Chemother 56: 4548–4549 [CrossRef] [PubMed].
    [Google Scholar]
  13. Lee Y. T., Kuo S. C., Chiang M. C., Yang S. P., Chen C. P., Chen T. L., Fung C. P.. ( 2012;). Emergence of carbapenem-resistant non-baumannii species of Acinetobacter harboring a blaOXA-51-like gene that is intrinsic to A. baumannii. Antimicrob Agents Chemother 56: 1124–1127 [CrossRef] [PubMed].
    [Google Scholar]
  14. Lim Y. M., Shin K. S., Kim J.. ( 2007;). Distinct antimicrobial resistance patterns and antimicrobial resistance-harboring genes according to genomic species of Acinetobacter isolates. J Clin Microbiol 45: 902–905 [CrossRef] [PubMed].
    [Google Scholar]
  15. Magiorakos A. P., Srinivasan A., Carey R. B., Carmeli Y., Falagas M. E., Giske C. G., Harbarth S., Hindler J. F., Kahlmeter G., other authors. ( 2012;). Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect 18: 268–281 [CrossRef] [PubMed].
    [Google Scholar]
  16. Martín-Lozano D., Cisneros J. M., Becerril B., Cuberos L., Prados T., Ortíz-Leyba C., Cañas E., Pachón J.. ( 2002;). Comparison of a repetitive extragenic palindromic sequence-based PCR method and clinical and microbiological methods for determining strain sources in cases of nosocomial Acinetobacter baumannii bacteremia. J Clin Microbiol 40: 4571–4575 [CrossRef] [PubMed].
    [Google Scholar]
  17. Mendes R. E., Farrell D. J., Sader H. S., Jones R. N.. ( 2010;). Comprehensive assessment of tigecycline activity tested against a worldwide collection of Acinetobacter spp. (2005–2009). Diagn Microbiol Infect Dis 68: 307–311 [CrossRef] [PubMed].
    [Google Scholar]
  18. Navon-Venezia S., Ben-Ami R., Carmeli Y.. ( 2005;). Update on Pseudomonas aeruginosa and Acinetobacter baumannii infections in the healthcare setting. Curr Opin Infect Dis 18: 306–313 [CrossRef] [PubMed].
    [Google Scholar]
  19. Nemec A., Krizova L., Maixnerova M., van der Reijden T.J.K., Deschaght P., Passet V., Vaneechoutte M., Brisse S., Dijkshoorn L.. ( 2011;). Genotypic and phenotypic characterization of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex with the proposal of Acinetobacter pittii sp. nov. (formerly Acinetobacter genomic species 3) and Acinetobacter nosocomialis sp. nov. (formerly Acinetobacter genomic species 13TU). Res Microbiol 162: 393–404 [CrossRef] [PubMed].
    [Google Scholar]
  20. Peleg A. Y., Franklin C., Walters L. J., Bell J. M., Spelman D. W.. ( 2006;). OXA-58 and IMP-4 carbapenem-hydrolyzing beta-lactamases in an Acinetobacter junii blood culture isolate from Australia. Antimicrob Agents Chemother 50: 399–400 [CrossRef] [PubMed].
    [Google Scholar]
  21. Poirel L., Nordmann P.. ( 2006;). Carbapenem resistance in Acinetobacter baumannii: mechanisms and epidemiology. Clin Microbiol Infect 12: 826–836 [CrossRef] [PubMed].
    [Google Scholar]
  22. Segal H., Garny S., Elisha B. G.. ( 2005;). Is IS(ABA-1) customized for Acinetobacter. FEMS Microbiol Lett 243: 425–429 [CrossRef] [PubMed].
    [Google Scholar]
  23. Teixeira A. B., Martins A. F., Barin J., Hermes D. M., Pitt C. P., Barth A. L.. ( 2013;). First report of carbapenem-resistant Acinetobacter nosocomialis isolates harboring ISAba1-blaOXA-23 genes in Latin America. J Clin Microbiol 51: 2739–2741 [CrossRef] [PubMed].
    [Google Scholar]
  24. Turton J. F., Ward M. E., Woodford N., Kaufmann M. E., Pike R., Livermore D. M., Pitt T. L.. ( 2006;). The role of ISAba1 in expression of OXA carbapenemase genes in Acinetobacter baumannii. FEMS Microbiol Lett 258: 72–77 [CrossRef] [PubMed].
    [Google Scholar]
  25. van den Broek P. J., van der Reijden T. J., van Strijen E., Helmig-Schurter A. V., Bernards A. T., Dijkshoorn L.. ( 2009;). Endemic and epidemic acinetobacter species in a university hospital: an 8-year survey. J Clin Microbiol 47: 3593–3599 [CrossRef] [PubMed].
    [Google Scholar]
  26. Wang H., Guo P., Sun H., Wang H., Yang Q., Chen M., Xu Y., Zhu Y.. ( 2007;). Molecular epidemiology of clinical isolates of carbapenem-resistant Acinetobacter spp. from Chinese hospitals. Antimicrob Agents Chemother 51: 4022–4028 [CrossRef] [PubMed].
    [Google Scholar]
  27. Wisplinghoff H., Edmond M. B., Pfaller M. A., Jones R. N., Wenzel R. P., Seifert H.. ( 2000;). Nosocomial bloodstream infections caused by Acinetobacter species in United States hospitals: clinical features, molecular epidemiology, and antimicrobial susceptibility. Clin Infect Dis 31: 690–697 [CrossRef] [PubMed].
    [Google Scholar]
  28. Wisplinghoff H., Paulus T., Lugenheim M., Stefanik D., Higgins P. G., Edmond M. B., Wenzel R. P., Seifert H.. ( 2012;). Nosocomial bloodstream infections due to Acinetobacter baumannii, Acinetobacter pittii and Acinetobacter nosocomialis in the United States. J Infect 64: 282–290 [CrossRef] [PubMed].
    [Google Scholar]
  29. Woodford N., Ellington M. J., Coelho J. M., Turton J. F., Ward M. E., Brown S., Amyes S. G., Livermore D. M.. ( 2006;). Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp. Int J Antimicrob Agents 27: 351–353 [CrossRef] [PubMed].
    [Google Scholar]
  30. Zarrilli R., Giannouli M., Tomasone F., Triassi M., Tsakris A.. ( 2009;). Carbapenem resistance in Acinetobacter baumannii: the molecular epidemic features of an emerging problem in health care facilities. J Infect Dev Ctries 3: 335–341 [CrossRef] [PubMed].
    [Google Scholar]
  31. Zavascki A. P., Carvalhaes C. G., Picão R. C., Gales A. C.. ( 2010;). Multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii: resistance mechanisms and implications for therapy. Expert Rev Anti Infect Ther 8: 71–93 [CrossRef] [PubMed].
    [Google Scholar]
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