1887

Abstract

The extragenital manifestation of infection frequently results in non-specific conjunctivitis among sexually active adults. The aims of the present study were to determine the prevalence of , to describe the distribution of serovars among patients with conjunctivitis and to characterize the relationship between the prevalence and patient demographics such as age and gender. A total of 245 conjunctival specimens were screened for DNA targeting the plasmid gene. Serovar determination of the -positive specimens was carried out by an PCR-based RFLP analysis method. Statistical analysis was done using a generalized linear model. was detected in 53 cases (21.6 %) of adult conjunctivitis. Molecular genotyping differentiated seven distinct urogenital serovars, the most prevalent being serovar E (16/53), followed by F (15/53), D (6/53), K (6/53), G (4/53), H (4/53) and J (2/53). Statistical analysis showed higher prevalence in the younger age groups, and this peaked at younger age in women than in men. The high prevalence of this pathogen found in ocular samples should alert ophthalmologists to focus on the role of in adult conjunctivitis. The serovar distribution indicated that ocular chlamydial infections usually have a genital source. Nevertheless, conjunctivitis might be the only sign of this sexually transmitted infection. Further comparative genotyping of in ocular and genital specimens might give more detailed epidemiological information about the aetiology of the disease.

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2015-09-01
2019-12-15
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References

  1. ECDC ( 2015;). Annual Epidemiological Report 2014 – Sexually Transmitted Infections, Including HIV and Blood-borne Viruses Stockholm: European Centre for Disease Prevention and Control;.
    [Google Scholar]
  2. Gao X., Chen X. S., Yin Y. P., Zhong M. Y., Shi M. Q., Wei W. H., Chen Q., Peeling R. W., Mabey D.. ( 2007;). Distribution study of Chlamydia trachomatis serovars among high-risk women in China performed using PCR-restriction fragment length polymorphism genotyping. J Clin Microbiol 45: 1185–1189 [CrossRef] [PubMed].
    [Google Scholar]
  3. Garland S. M., Malatt A., Tabrizi S., Grando D., Lees M. I., Andrew J. H., Taylor H. R.. ( 1995;). Chlamydia trachomatis conjunctivitis. Prevalence and association with genital tract infection. Med J Aust 162: 363–366.
    [Google Scholar]
  4. Haller-Schober E. M., El-Shabrawi Y.. ( 2002;). Chlamydial conjunctivitis (in adults), uveitis, and reactive arthritis, including SARA. Best Pract Res Clin Obstet Gynaecol 16: 815–828 [CrossRef] [PubMed].
    [Google Scholar]
  5. Harrell F. E. Jr, Lee K. L., Mark D. B.. ( 1996;). Multivariable prognostic models: issues in developing models, evaluating assumptions and adequacy, and measuring and reducing errors. Stat Med 15: 361–387 [CrossRef] [PubMed].
    [Google Scholar]
  6. HPA ( 2008;). Sexually Transmitted Infections Surveillance in Europe Annual Report No. 3 London: Health Protection Agency Centre for Infections;.
    [Google Scholar]
  7. Isobe K., Aoki K., Itoh N., Ohno S., Takashima I., Hashimoto N.. ( 1996;). Serotyping of Chlamydia trachomatis from inclusion conjunctivitis by polymerase chain reaction and restriction fragment length polymorphism analysis. Jpn J Ophthalmol 40: 279–285.
    [Google Scholar]
  8. Johnson A. M., Mercer C. H., Erens B., Copas A. J., McManus S., Wellings K., Fenton K. A., Korovessis C., Macdowall W., other authors. ( 2001;). Sexual behaviour in Britain: partnerships, practices, and HIV risk behaviours. Lancet 358: 1835–1842 [CrossRef] [PubMed].
    [Google Scholar]
  9. Kese D., Potocnik M., Maticic M., Kogoj R.. ( 2011;). Genotyping of Chlamydia trachomatis directly from urogenital and conjunctiva samples using an ompA gene pyrosequencing-based assay. FEMS Immunol Med Microbiol 63: 210–216 [CrossRef] [PubMed].
    [Google Scholar]
  10. Lan J., Walboomers J. M., Roosendaal R., van Doornum G. J., MacLaren D. M., Meijer C. J., van den Brule A. J.. ( 1993;). Direct detection and genotyping of Chlamydia trachomatis in cervical scrapes by using polymerase chain reaction and restriction fragment length polymorphism analysis. J Clin Microbiol 31: 1060–1065.
    [Google Scholar]
  11. Lima H. E., Oliveira M. B., Valente B. G., Afonso D. A., Darocha W. D., Souza M. C., Alvim T. C., Barbosa-Stancioli E. F., Noronha F. S.. ( 2007;). Genotyping of Chlamydia trachomatis from endocervical specimens in Brazil. Sex Transm Dis 34: 709–717 [CrossRef] [PubMed].
    [Google Scholar]
  12. Lysén M., Österlund A., Rubin C. J., Persson T., Persson I., Herrmann B.. ( 2004;). Characterization of ompA genotypes by sequence analysis of DNA from all detected cases of Chlamydia trachomatis infections during 1 year of contact tracing in a Swedish county. J Clin Microbiol 42: 1641–1647 [CrossRef] [PubMed].
    [Google Scholar]
  13. Mahony J. B., Luinstra K. E., Sellors J. W., Chernesky M. A.. ( 1993;). Comparison of plasmid- and chromosome-based polymerase chain reaction assays for detecting Chlamydia trachomatis nucleic acids. J Clin Microbiol 31: 1753–1758.
    [Google Scholar]
  14. Petrovay F., Balla E., Németh I., Gönczöl E.. ( 2009;). Genotyping of Chlamydia trachomatis from the endocervical specimens of high-risk women in Hungary. J Med Microbiol 58: 760–764 [CrossRef] [PubMed].
    [Google Scholar]
  15. Postema E. J., Remeijer L., van der Meijden W. I.. ( 1996;). Epidemiology of genital chlamydial infections in patients with chlamydial conjunctivitis; a retrospective study. Genitourin Med 72: 203–205.
    [Google Scholar]
  16. Quirke M., Cullinane A.. ( 2008;). Recent trends in chlamydial and gonococcal conjunctivitis among neonates and adults in an Irish hospital. Int J Infect Dis 12: 371–373 [CrossRef] [PubMed].
    [Google Scholar]
  17. R Core Team ( 2011;). R: A Language and Environment for Statistical Computing version 2.13.0 Vienna, Austria: R Foundation for Statistical Computing;.
    [Google Scholar]
  18. Rao S. K., Madhavan H. N., Padmanabhan P., Lakshmi G. S., Natarajan K., Garg D.. ( 1996;). Ocular chlamydial infections. Clinicomicrobiological correlation. Cornea 15: 62–65 [CrossRef] [PubMed].
    [Google Scholar]
  19. Reischl U., Straube E., Unemo M.. ( 2009;). The Swedish new variant of Chlamydia trachomatis (nvCT) remains undetected by many European laboratories as revealed in the recent PCR/NAT ring trial organised by INSTAND e.V. Germany. Euro Surveill 14: 19302.
    [Google Scholar]
  20. Rours I. G., Hammerschlag M. R., Ott A., De Faber T. J., Verbrugh H. A., de Groot R., Verkooyen R. P.. ( 2008;). Chlamydia trachomatis as a cause of neonatal conjunctivitis in Dutch infants. Pediatrics 121: e321–e326 [CrossRef] [PubMed].
    [Google Scholar]
  21. Simms I., Talebi A., Rhia J., Horner P., French R. S., Sarah R., Macintosh M.. ( 2009;). The English National Chlamydia Screening Programme: variations in positivity in 2007/2008. Sex Transm Dis 36: 522–527 [CrossRef] [PubMed].
    [Google Scholar]
  22. Stenberg K., Mårdh P. A.. ( 1990;). Chlamydial conjunctivitis in neonates and adults. History, clinical findings and follow-up. Acta Ophthalmol (Copenh) 68: 651–657 [CrossRef] [PubMed].
    [Google Scholar]
  23. Stenberg K., Mårdh P. A.. ( 1991;). Genital infection with Chlamydia trachomatis in patients with chlamydial conjunctivitis: unexplained results. Sex Transm Dis 18: 1–4 [CrossRef] [PubMed].
    [Google Scholar]
  24. Taylor H. R., Burton M. J., Haddad D., West S., Wright H.. ( 2014;). Trachoma. Lancet 384: 2142–2152 [CrossRef] [PubMed].
    [Google Scholar]
  25. Wadsworth J., Johnson A. M., Wellings K., Field J.. ( 1996;). What's in a mean? – An examination of the inconsistency between men and women in reporting sexual partnerships. J R Stat Soc Ser A Stat Soc 159: 111–123 [CrossRef].
    [Google Scholar]
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