The 16 serovar Enteritidis (. Enteritidis) typing phages (SETPs) used in the Laboratory of Enteric Pathogens (Health Protection Agency, London, UK) phage-typing scheme have not previously been characterized in detail. We have examined the adsorption properties of the phages with respect to a number of serovars and defined phage morphology with electron microscopy. PFGE was used to estimate overall genome size and banding patterns generated by electrophoresis following restriction endonuclease digestion of the genome with dIII were compared. PCR amplification and sequencing of selected genes was performed. The 16 phages comprise three morphotypes, (SETP1, 8, 10, 14, 15 and 16), (SETP3, 5, 7, 11, 12 and 13) and (SETP2, 4, 6 and 9). All and , but not adsorbed to the O12 lipopolysaccharide antigen of serogroups B (4,12) and D (9,12). The genome sizes for the and (PFGE-A) were approximately 42 kb. The genome size for SETP2, 4 and 9 was 36.5 kb, and for myovirus SETP6 was 27 kb. dIII digestion of phage DNA produced 9 distinct patterns of 8 to 11 bands. Relationships between phages based on digest patterns were consistent with those defined by morphology. The had homologues of several P22 genes while the had homologues of several genes present in the sequenced siphovirus SETP3 (EF177456). This study represents an initial step in characterizing the molecular basis that underlies the widely used . Enteritidis typing scheme.


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vol. , part 1, pp. 86 - 93

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Probes used to detect putative P22 gene homologues.

Consensus primers used to amplify P22 gene homologues.

Consensus primers used to amplify putative SETP3 gene homologues.

Phage typing of non-Enteritidis serovars.

Nucleotide sequence accession numbers.

Summary of P22 BLASTresults.

Prophage induction from various Enteritidis phage types.

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