1887

Abstract

In Japan, publicly subsidized serotype b vaccines became available in 2011; consequently, the incidence of invasive infection in paediatric patients of less than 5 years of age decreased dramatically. In 2013, the first case of serotype f (Hif) meningitis in a Japanese infant was reported, and another case of Hif meningitis in a Japanese infant was observed in 2013. We experienced a fatal paediatric case of Hif bacteraemia in 2004; therefore, we conducted an analysis of the three Hif strains isolated from these three Japanese children with invasive Hif infections. All three strains were β-lactamase-non-producing, ampicillin-sensitive strains, with MICs of 1 µg ml or less. However, one of the three strains showed slightly elevated MICs for ampicillin (1 µg ml), cefotaxime (0.25 µg ml) and meropenem (0.13 µg ml). A molecular analysis by multilocus sequence typing identified all three strains as sequence type (ST) 124, which is a predominant invasive Hif strain in many countries. I-digested PFGE showed variable DNA fragmentation patterns among the strains, suggesting that some highly virulent strains have originated from a single ST124 clone and caused invasive Hif infections in Japan. Additional studies are needed to determine the factors that have led to the clonal expansion of virulent ST124 strains.

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2015-04-01
2019-12-10
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References

  1. Campos J., Román F., Pérez-Vázquez M., Aracil B., Oteo J., Cercenado E..Spanish Study Group for H. influenzae type f ( 2003;). Antibiotic resistance and clinical significance of Haemophilus influenzae type f. . J Antimicrob Chemother 52:, 961–966. [CrossRef][PubMed]
    [Google Scholar]
  2. CLSI (2012). Performance standards for antimicrobial susceptibility testing; 17th informational supplement M100-S21. Wayne, PA: Clinical and Laboratory Standards Institute.
  3. Falla T. J., Crook D. W., Brophy L. N., Maskell D., Kroll J. S., Moxon E. R.. ( 1994;). PCR for capsular typing of Haemophilus influenzae. . J Clin Microbiol 32:, 2382–2386.[PubMed]
    [Google Scholar]
  4. Giufrè M., Cardines R., Accogli M., Pardini M., Cerquetti M.. ( 2013;). Identification of Haemophilus influenzae clones associated with invasive disease a decade after introduction of H. influenzae serotype b vaccination in Italy. . Clin Vaccine Immunol 20:, 1223–1229. [CrossRef][PubMed]
    [Google Scholar]
  5. Hasegawa K., Kobayashi R., Takada E., Ono A., Chiba N., Morozumi M., Iwata S., Sunakawa K., Ubukata K..Nationwide Surveillance for Bacterial Meningitis ( 2006;). High prevalence of type b β-lactamase-non-producing ampicillin-resistant Haemophilus influenzae in meningitis: the situation in Japan where Hib vaccine has not been introduced. . J Antimicrob Chemother 57:, 1077–1082. [CrossRef][PubMed]
    [Google Scholar]
  6. Ishiwada N., Hishiki H., Nagasawa K., Naito S., Sato Y., Chang B., Sasaki Y., Kimura K., Ohnishi M., Shibayama K.. ( 2014;). The incidence of pediatric invasive Haemophilus influenzae and pneumococcal disease in Chiba prefecture, Japan before and after the introduction of conjugate vaccines. . Vaccine 32:, 5425–5431. [CrossRef][PubMed]
    [Google Scholar]
  7. Ladhani S., Slack M. P., Heath P. T., von Gottberg A., Chandra M., Ramsay M. E..European Union Invasive Bacterial Infection Surveillance participants ( 2010;). Invasive Haemophilus influenzae disease, Europe, 1996–2006. . Emerg Infect Dis 16:, 455–463. [CrossRef][PubMed]
    [Google Scholar]
  8. Ladhani S. N., Collins S., Vickers A., Litt D. J., Crawford C., Ramsay M. E., Slack M. P. E.. ( 2012;). Invasive Haemophilus influenzae serotype e and f disease, England and Wales. . Emerg Infect Dis 18:, 725–732. [CrossRef][PubMed]
    [Google Scholar]
  9. MacNeil J. R., Cohn A. C., Farley M., Mair R., Baumbach J., Bennett N., Gershman K., Harrison L. H., Lynfield R. et al. ( 2011;). Current epidemiology and trends in invasive Haemophilus influenzae disease – United States, 1989–2008. . Clin Infect Dis 53:, 1230–1236. [CrossRef][PubMed]
    [Google Scholar]
  10. Meats E., Feil E. J., Stringer S., Cody A. J., Goldstein R., Kroll J. S., Popovic T., Spratt B. G.. ( 2003;). Characterization of encapsulated and noncapsulated Haemophilus influenzae and determination of phylogenetic relationships by multilocus sequence typing. . J Clin Microbiol 41:, 1623–1636. [CrossRef][PubMed]
    [Google Scholar]
  11. Mitsuda T., Kuroki H., Ishikawa N., Imagawa T., Ito S., Miyamae T., Mori M., Uehara S., Yokota S.. ( 1999;). Molecular epidemiological study of Haemophilus influenzae serotype b strains obtained from children with meningitis in Japan. . J Clin Microbiol 37:, 2548–2552.[PubMed]
    [Google Scholar]
  12. Murayama K., Nagasaka H., Tsuruoka T., Omata Y., Horie H., Tregoning S., Thorburn D. R., Takayanagi M., Ohtake A.. ( 2009;). Intractable secretory diarrhea in a Japanese boy with mitochondrial respiratory chain complex I deficiency. . Eur J Pediatr 168:, 297–302. [CrossRef][PubMed]
    [Google Scholar]
  13. Omikunle A., Takahashi S., Ogilvie C. L., Wang Y., Rodriguez C. A., St Geme J. W. III, Adderson E. E.. ( 2002;). Limited genetic diversity of recent invasive isolates of non-serotype b encapsulated Haemophilus influenzae. . J Clin Microbiol 40:, 1264–1270. [CrossRef][PubMed]
    [Google Scholar]
  14. Oohara A., Shimizu H., Hara R., Fukushima R., Sakon T., Funabiki T., Takahashi S., Hishiki H., Ishiwada N.. ( 2014;). [A case of Haemophilus influenzae type f meningitis after three doses of Hib vaccinations]. . J Jpn Pediatr Soc 118:, 1079–1084 (in Japanese).
    [Google Scholar]
  15. Shuel M., Hoang L., Law D. K., Tsang R.. ( 2011;). Invasive Haemophilus influenzae in British Columbia: non-Hib and non-typeable strains causing disease in children and adults. . Int J Infect Dis 15:, e167–e173. [CrossRef][PubMed]
    [Google Scholar]
  16. Sill M. L., Law D. K., Zhou J., Skinner S., Wylie J., Tsang R. S.. ( 2007;). Population genetics and antibiotic susceptibility of invasive Haemophilus influenzae in Manitoba, Canada, from 2000 to 2006. . FEMS Immunol Med Microbiol 51:, 270–276. [CrossRef][PubMed]
    [Google Scholar]
  17. Ubukata K., Chiba N., Morozumi M., Iwata S., Sunakawa K..Working Group of Nationwide Surveillance for Bacterial Meningitis ( 2013;). Longitudinal surveillance of Haemophilus influenzae isolates from pediatric patients with meningitis throughout Japan, 2000–2011. . J Infect Chemother 19:, 34–41. [CrossRef][PubMed]
    [Google Scholar]
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