1887

Abstract

is an oral bacterium that is associated with inflammatory bowel disease (IBD). This study examined the impact of pH and bile on the growth of oral strains isolated from patients with IBD and controls. The growth of 58 strains on horse blood agar (HBA) plates following exposure to media with various pH values for different time points was examined. Furthermore, the growth of strains on HBA plates containing different concentrations of ox bile was investigated. Following exposure to pH 2 for 30 min, none of the 58 oral strains grew on HBA plates. Following exposure to pH 3.5 for 30 min, only four of 20 oral strains examined grew on HBA plates, with a log c.f.u. reduction of 0.7–2.5 compared to the same strains without low pH exposure. Exposure to pH 5 for 120 min had minimal effects on growth. Approximately half of the oral strains (55.2 %, 32/58) grew on HBA containing 2 % bile. Bile inhibited the growth of in a dose- and strain-dependent manner. These data suggest that both bacterial and intestinal environmental factors may play a role in the determination of colonization in the different parts of the gastrointestinal tract and that increased gastric pH and reduced intestinal bile may be risk factors for increased gastric and intestinal colonization.

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2015-04-01
2019-08-25
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References

  1. Chiang J. Y. . ( 2009; ). Bile acids: regulation of synthesis. . J Lipid Res 50:, 1955–1966. [CrossRef] [PubMed]
    [Google Scholar]
  2. Cosnes J. , Gower-Rousseau C. , Seksik P. , Cortot A. . ( 2011; ). Epidemiology and natural history of inflammatory bowel diseases. . Gastroenterology 140:, 1785–1794. [CrossRef] [PubMed]
    [Google Scholar]
  3. Engberg J. , On S. L. W. , Harrington C. S. , Gerner-Smidt P. . ( 2000; ). Prevalence of Campylobacter, Arcobacter, Helicobacter, and Sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for campylobacters . . J Clin Microbiol 38:, 286–291.[PubMed]
    [Google Scholar]
  4. Fox E. M. , Raftery M. , Goodchild A. , Mendz G. L. . ( 2007; ). Campylobacter jejuni response to ox-bile stress. . FEMS Immunol Med Microbiol 49:, 165–172. [CrossRef] [PubMed]
    [Google Scholar]
  5. Garrity G. M. , Bell J. A. , Lilburn T. . ( 2005; ). Class V. Epsilonproteobacteria class. nov. . In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1145–1194. Edited by Brenner D. J. , Krieg N. R. , Staley J. T. . . New York:: Springer;. [CrossRef]
    [Google Scholar]
  6. Gonzalez-Escobedo G. , Marshall J. M. , Gunn J. S. . ( 2011; ). Chronic and acute infection of the gall bladder by Salmonella Typhi: understanding the carrier state. . Nat Rev Microbiol 9:, 9–14. [CrossRef] [PubMed]
    [Google Scholar]
  7. Guarner F. , Malagelada J. R. . ( 2003; ). Gut flora in health and disease. . Lancet 361:, 512–519. [CrossRef] [PubMed]
    [Google Scholar]
  8. Humphrey S. P. , Williamson R. T. . ( 2001; ). A review of saliva: normal composition, flow, and function. . J Prosthet Dent 85:, 162–169. [CrossRef] [PubMed]
    [Google Scholar]
  9. Imhoff J. . ( 2005; ). Enterobacteriales . . In Bergey’s Manual of Systematic Bacteriology, pp. 587–850. Edited by Brenner D. J. , Krieg N. R. , Staley J. T. , Garrity G. M. , Boone D. R. , De Vos P. , Goodfellow M. , Rainey F. A. , Schleifer K.-H. . . New York:: Springer;. [CrossRef]
    [Google Scholar]
  10. Ismail Y. , Mahendran V. , Octavia S. , Day A. S. , Riordan S. M. , Grimm M. C. , Lan R. , Lemberg D. , Tran T. A. T. , Zhang L. . ( 2012; ). Investigation of the enteric pathogenic potential of oral Campylobacter concisus strains isolated from patients with inflammatory bowel disease. . PLoS ONE 7:, e38217. [CrossRef] [PubMed]
    [Google Scholar]
  11. Lastovica A. J. . ( 2009; ). Clinical relevance of Campylobacter concisus isolated from pediatric patients. . J Clin Microbiol 47:, 2360. [CrossRef] [PubMed]
    [Google Scholar]
  12. Lee H. , Ma R. , Grimm M. C. , Riordan S. M. , Lan R. , Zhong L. , Raftery M. , Zhang L. . ( 2014; ). Examination of the anaerobic growth of Campylobacter concisus strains. . Int J Microbiol 2014:, 476047. [CrossRef] [PubMed]
    [Google Scholar]
  13. Mahendran V. , Riordan S. M. , Grimm M. C. , Tran T. A. T. , Major J. , Kaakoush N. O. , Mitchell H. , Zhang L. . ( 2011; ). Prevalence of Campylobacter species in adult Crohn’s disease and the preferential colonization sites of Campylobacter species in the human intestine. . PLoS ONE 6:, e25417. [CrossRef] [PubMed]
    [Google Scholar]
  14. Mahendran V. , Tan Y. S. , Riordan S. M. , Grimm M. C. , Day A. S. , Lemberg D. A. , Octavia S. , Lan R. , Zhang L. . ( 2013; ). The prevalence and polymorphisms of zonula occluden toxin gene in multiple Campylobacter concisus strains isolated from saliva of patients with inflammatory bowel disease and controls. . PLoS ONE 8:, e75525. [CrossRef] [PubMed]
    [Google Scholar]
  15. Man S. M. , Zhang L. , Day A. S. , Leach S. T. , Lemberg D. A. , Mitchell H. . ( 2010; ). Campylobacter concisus and other Campylobacter species in children with newly diagnosed Crohn’s disease. . Inflamm Bowel Dis 16:, 1008–1016. [CrossRef] [PubMed]
    [Google Scholar]
  16. Mukhopadhya I. , Thomson J. M. , Hansen R. , Berry S. H. , El-Omar E. M. , Hold G. L. . ( 2011; ). Detection of Campylobacter concisus and other Campylobacter species in colonic biopsies from adults with ulcerative colitis. . PLoS ONE 6:, e21490. [CrossRef] [PubMed]
    [Google Scholar]
  17. Nielsen H. L. , Ejlertsen T. , Engberg J. , Nielsen H. . ( 2013; ). High incidence of Campylobacter concisus in gastroenteritis in North Jutland, Denmark: a population-based study. . Clin Microbiol Infect 19:, 445–450. [CrossRef] [PubMed]
    [Google Scholar]
  18. Press A. G. , Hauptmann I. A. , Hauptmann L. , Fuchs B. , Fuchs M. , Ewe K. , Ramadori G. . ( 1998; ). Gastrointestinal pH profiles in patients with inflammatory bowel disease. . Aliment Pharmacol Ther 12:, 673–678. [CrossRef] [PubMed]
    [Google Scholar]
  19. Stiehl A. , Raedsch R. , Rudolph G. . ( 1988; ). Ileal excretion of bile acids: comparison with biliary bile composition and effect of ursodeoxycholic acid treatment. . Gastroenterology 94:, 1201–1206.[PubMed]
    [Google Scholar]
  20. Tanner A. C. R. , Badger S. , Lai C.-H. , Listgarten M. A. , Visconti R. A. , Socransky S. S. . ( 1981; ). Wolinella gen. nov., Wolinella succinogenes (Vibrio succinogenes Wolin et al.) comb. nov., and description of Bacteroides gracilis sp. nov., Wolinella recta sp. nov., Campylobacter concisus sp. nov., and Eikenella corrodens from humans with periodontal disease. . Int J Syst Bacteriol 31:, 432–445. [CrossRef]
    [Google Scholar]
  21. Vandamme P. , Dewhirst F. E. , Paster B. J. , On S. L. . ( 2005; ). Genus I. Campylobacter . . In Bergey’s Manual of Systematic Bacteriology. vol. 2:, pp. 1147–1160. Edited by Garrity G. M. , Brenner D. J. , Krieg N. R. , Staley J. T. . . New York:: Springer;. [CrossRef]
    [Google Scholar]
  22. Xu H.-S. , Roberts N. , Singleton F. L. , Attwell R. W. , Grimes D. J. , Colwell R. R. . ( 1982; ). Survival and viability of nonculturable Escherichia coli and Vibrio cholerae in the estuarine and marine environment. . Microb Ecol 8:, 313–323. [CrossRef] [PubMed]
    [Google Scholar]
  23. Zhang L. , Man S. M. , Day A. S. , Leach S. T. , Lemberg D. A. , Dutt S. , Stormon M. , Otley A. , O’Loughlin E. V. et al. ( 2009; ). Detection and isolation of Campylobacter species other than C. jejuni from children with Crohn’s disease. . J Clin Microbiol 47:, 453–455. [CrossRef] [PubMed]
    [Google Scholar]
  24. Zhang L. , Budiman V. , Day A. S. , Mitchell H. , Lemberg D. A. , Riordan S. M. , Grimm M. , Leach S. T. , Ismail Y. . ( 2010; ). Isolation and detection of Campylobacter concisus from saliva of healthy individuals and patients with inflammatory bowel disease. . J Clin Microbiol 48:, 2965–2967. [CrossRef] [PubMed]
    [Google Scholar]
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