1887

Abstract

We previously reported a shift in the electrophoretic type (ET) of invasive MenC in Canada from predominantly ET-15 to ET-37 in the post-MenC conjugate vaccine period. This study sought to confirm this trend by examining all culture-confirmed invasive MenC case isolates in Canada in the period from 1 January 2009 to 31 December 2013. Of the 50 MenC isolates, 18 belonged to ET-15, 28 belonged to ET-37 (but not ET-15), and four belonged to other clonal types. Analysis of the serotype and serosubtype antigens, and gene sequences provided data to show that invasive MenC belonging to ET-15 and ET-37 were two very different subpopulations within the ST-11 clonal complex. Sequence analysis of the genes suggested that 12 different types of factor H-binding protein were found among the ET-15 isolates while 86 % of ET-37 isolates were found to have genes predicted to encode peptide 22. The gene in 12 MenC isolates was disrupted due to IS insertion and 11 of these 12 isolates belonged to ET-15. Ten per cent of the invasive MenC were found to have a frame-shift mutation in their genes that predicted no fHbp produced. Significant diversity and frame-shift mutations of genes were found in invasive MenC strains in Canada.

Erratum
This article contains a correction applying to the following content:
Erratum
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000006
2015-02-01
2019-12-14
Loading full text...

Full text loading...

/deliver/fulltext/jmm/64/2/174.html?itemId=/content/journal/jmm/10.1099/jmm.0.000006&mimeType=html&fmt=ahah

References

  1. Abdillahi H., Poolman J. T.. ( 1987;). Whole-cell ELISA for typing Neisseria meningitidis with monoclonal antibodies. . FEMS Microbiol Lett 48:, 367–371. [CrossRef]
    [Google Scholar]
  2. Ashton F. E., Ryan J. A., Borczyk A., Caugant D. A., Mancino L., Huang D.. ( 1991;). Emergence of a virulent clone of Neisseria meningitidis serotype 2a that is associated with meningococcal group C disease in Canada. . J Clin Microbiol 29:, 2489–2493.[PubMed]
    [Google Scholar]
  3. Caugant D. A., Bøvre K., Gaustad P., Bryn K., Holten E., Høiby E. A., Frøholm L. O.. ( 1986;). Multilocus genotypes determined by enzyme electrophoresis of Neisseria meningitidis isolated from patients with systemic disease and from healthy carriers. . J Gen Microbiol 132:, 641–652.[PubMed]
    [Google Scholar]
  4. Claus H., Elias J., Meinhardt C., Frosch M., Vogel U.. ( 2007;). Deletion of the meningococcal fetA gene used for antigen sequence typing of invasive and commensal isolates from Germany: frequencies and mechanisms. . J Clin Microbiol 45:, 2960–2964. [CrossRef][PubMed]
    [Google Scholar]
  5. De Wals P., Deceuninck G., Lefebvre B., Boulianne N., De Serres G.. ( 2011;). Effectiveness of serogroup C meningococcal conjugate vaccine: a 7-year follow-up in Quebec, Canada. . Pediatr Infect Dis J 30:, 566–569. [CrossRef][PubMed]
    [Google Scholar]
  6. Elias J., Vogel U.. ( 2007;). IS1301 fingerprint analysis of Neisseria meningitidis strains belonging to the ET-15 clone. . J Clin Microbiol 45:, 159–167. [CrossRef][PubMed]
    [Google Scholar]
  7. Frasch C. E., Zollinger W. D., Poolman J. T.. ( 1985;). Serotype antigens of Neisseria meningitidis and a proposed scheme for designation of serotypes. . Rev Infect Dis 7:, 504–510. [CrossRef][PubMed]
    [Google Scholar]
  8. Harrison O. B., Brueggemann A. B., Caugant D. A., van der Ende A., Frosch M., Gray S., Heuberger S., Krizova P., Olcen P. et al. ( 2011;). Molecular typing methods for outbreak detection and surveillance of invasive disease caused by Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae, a review. . Microbiology 157:, 2181–2195. [CrossRef][PubMed]
    [Google Scholar]
  9. Hill A. B.. ( 1977;). A Short Textbook of Medical Statistics. London:: Hodder & Stoughton;.
    [Google Scholar]
  10. Hong E., Giorgini D., Deghmane A. E., Taha M. K.. ( 2012;). Functional impacts of the diversity of the meningococcal factor H binding protein. . Vaccine 31:, 183–189. [CrossRef][PubMed]
    [Google Scholar]
  11. Jamieson F. B., Rawte P., Deeks S. L., Zhou J., Law D. K. S., Deng S., Tsang R. S. W.. ( 2013;). Genetic and antigenic characterization of invasive endemic serogroup B Neisseria meningitidis from Ontario, Canada, in 2001–2010. . J Med Microbiol 62:, 46–55. [CrossRef][PubMed]
    [Google Scholar]
  12. Jelfs J., Munro R., Ashton F. E., Caugant D. A.. ( 2000;). Genetic characterization of a new variant within the ET-37 complex of Neisseria meningitidis associated with outbreaks in various parts of the world. . Epidemiol Infect 125:, 285–298. [CrossRef][PubMed]
    [Google Scholar]
  13. Kinlin L. M., Jamieson F., Brown E. M., Brown S., Rawte P., Dolman S., Drews S. J., Fisman D. N.. ( 2009;). Rapid identification of herd effects with the introduction of serogroup C meningococcal conjugate vaccine in Ontario, Canada, 2000–2006. . Vaccine 27:, 1735–1740. [CrossRef][PubMed]
    [Google Scholar]
  14. Law D. K. S., Stoltz J., Henderson A. M., Tsang R. S. W.. ( 2005;). Antigenic and genetic characterization of serogroup C meningococci isolated from invasive meningococcal disease cases in Canada from 1999 to 2003. . Can J Microbiol 51:, 523–530. [CrossRef][PubMed]
    [Google Scholar]
  15. Law D. K. S., Zhou J., Deng S., Hoang L., Tyrrell G., Horsman G., Wylie J., Tsang R. S. W.. ( 2014;). Determination of serotyping antigens, clonal analysis and genetic characterization of the 4CMenB vaccine antigen genes in invasive Neisseria meningitidis from western Canada, 2009 to 2013. . J Med Microbiol 63:, 1490–1499. [CrossRef][PubMed]
    [Google Scholar]
  16. Li Y. A., Tsang R., Desai S., Deehan H.. ( 2014;). Enhanced surveillance of invasive meningococcal disease in Canada, 2006–2011. . Can Commun Dis Rep 40:, 160–169.
    [Google Scholar]
  17. Lucidarme J., Comanducci M., Findlow J., Gray S. J., Kaczmarski E. B., Guiver M., Vallely P. J., Oster P., Pizza M. et al. ( 2010;). Characterization of fHbp, nhba (gna2132), nadA, porA, and sequence type in group B meningococcal case isolates collected in England and Wales during January 2008 and potential coverage of an investigational group B meningococcal vaccine. . Clin Vaccine Immunol 17:, 919–929. [CrossRef][PubMed]
    [Google Scholar]
  18. Lucidarme J., Tan L., Exley R. M., Findlow J., Borrow R., Tang C. M.. ( 2011;). Characterization of Neisseria meningitidis isolates that do not express the virulence factor and vaccine antigen factor H binding protein. . Clin Vaccine Immunol 18:, 1002–1014. [CrossRef][PubMed]
    [Google Scholar]
  19. Maiden M. C. J., Bygraves J. A., Feil E., Morelli G., Russell J. E., Urwin R., Zhang Q., Zhou J., Zurth K. et al. ( 1998;). Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. . Proc Natl Acad Sci U S A 95:, 3140–3145. [CrossRef][PubMed]
    [Google Scholar]
  20. Marsh J. W., O’Leary M. M., Shutt K. A., Harrison L. H.. ( 2007;). Deletion of fetA gene sequences in serogroup B and C Neisseria meningitidis isolates. . J Clin Microbiol 45:, 1333–1335. [CrossRef][PubMed]
    [Google Scholar]
  21. NACI ( 2013;). An Advisory Committee Statement (ACS) update on the use of quadrivalent conjugate meningococcal vaccines. . Can Commun Dis Rep 39:, 1–40. [CrossRef]
    [Google Scholar]
  22. Pollard A. J., Tam T. W. S..National Immunization Committee on Immunization ( 2011;). An Advisory Committee Statement on the recommended use of meningococcal vaccines. . Can Commun Dis Rep 27:, 2–36.
    [Google Scholar]
  23. Richmond P. C., Marshall H. S., Nissen M. D., Jiang Q., Jansen K. U., Garcés-Sánchez M., Martinón-Torres F., Beeslaar J., Szenborn L. et al. ( 2012;). Safety, immunogenicity, and tolerability of meningococcal serogroup B bivalent recombinant lipoprotein 2086 vaccine in healthy adolescents: a randomised, single-blind, placebo-controlled, phase 2 trial. . Lancet Infect Dis 12:, 597–607. [CrossRef][PubMed]
    [Google Scholar]
  24. Rosenstein N. E., Perkins B. A., Stephens D. S., Popovic T., Hughes J. M.. ( 2001;). Meningococcal disease. . N Engl J Med 344:, 1378–1388. [CrossRef][PubMed]
    [Google Scholar]
  25. Santolaya M. E., O’Ryan M. L., Valenzuela M. T., Prado V., Vergara R., Muñoz A., Toneatto D., Graña G., Wang H. et al. ( 2012;). Immunogenicity and tolerability of a multicomponent meningococcal serogroup B (4CMenB) vaccine in healthy adolescents in Chile: a phase 2b/3 randomised, observer-blind, placebo-controlled study. . Lancet 379:, 617–624. [CrossRef][PubMed]
    [Google Scholar]
  26. Sloan A. M., Henderson A. M., Tsang R. S. W.. ( 2008;). Characterization of serogroup A Neisseria meningitidis from invasive meningococcal disease cases in Canada between 1979 and 2006: epidemiological links to returning travellers. . Can J Infect Dis Med Microbiol 19:, 227–232.[PubMed]
    [Google Scholar]
  27. Vogel U., Claus H., Frosch M., Caugant D. A.. ( 2000;). Molecular basis for distinction of the ET-15 clone within the ET-37 complex of Neisseria meningitidis.. J Clin Microbiol 38:, 941–942.[PubMed]
    [Google Scholar]
  28. Zhou J., Jamieson F., Dolman S., Hoang L. M., Rawte P., Tsang R. S. W.. ( 2012a;). Genetic and antigenic analysis of invasive serogroup C Neisseria meningitidis in Canada: a decrease in the electrophoretic type (ET)-15 clonal type and an increase in the proportion of isolates belonging to the ET-37 (but not ET-15) clonal type during the period from 2002 to 2009. . Can J Infect Dis Med Microbiol 23:, e55–e59.[PubMed]
    [Google Scholar]
  29. Zhou J., Lefebvre B., Deng S., Gilca R., Deceuninck G., Law D. K. S., De Wals P., Tsang R. S. W.. ( 2012b;). Invasive serogroup B Neisseria meningitidis in Quebec, Canada, 2003 to 2010: persistence of the ST-269 clone since it first emerged in 2003. . J Clin Microbiol 50:, 1545–1551. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000006
Loading
/content/journal/jmm/10.1099/jmm.0.000006
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error