1887

Abstract

SUMMARY

Antibody to δ-lysin of was produced in rabbits given injections of purified antigen. Removal of normal serum inhibitors was effected by ammonium-sulphate fractionation and ion-exchange chromato-graphy. The purified anti-δ-lysin showed a single line of precipitation with purified antigen in Ouchterlony agar gel-diffusion tests and when subjected to immunoelectrophoresis. The line of precipitation also coincided with the zone of haemolysis caused by δ-lysin when the agar gel-diffusion test was performed in blood-agarose plates.

A plot of the data obtained in a quantitative precipitin test performed with purified antibody and δ-lysin showed a characteristic curve confirming their interaction; in control tests with non-immune sera in place of δ-antibody, no precipitation was evident.

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/content/journal/jmm/10.1099/00222615-7-4-411
1974-11-01
2022-05-21
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References

  1. Cairo J. D., Wiseman G. M. 1970; Purification of the delta toxin of Staphylococcus aureus. Can. J. Microbiol 16:703
    [Google Scholar]
  2. Campbell D. H., Garvey S., Cremer E., Sussdorf H. 1970 Methods in Immunology 2nd ed New York:
    [Google Scholar]
  3. Donahue J. A. 1969; Antistaphylococcal hemolysins and delta hemolysin inhibitor in adult human serum. Can. J. Microbiol 15:957
    [Google Scholar]
  4. Fackrell H. B., Wiseman G. M. 1974; Production and purification of the gamma hemolysin of Staphylococcus aureus “ Smith ” 5R. J. gen. Microbiol in press
    [Google Scholar]
  5. Gladstone G. P., Yoshida A. 1967; The cytopathic action of purified staphylococcal delta haemolysin. Brit. J. exp. Path 48:11
    [Google Scholar]
  6. Gomori G. 1955 In Methods in enzymology edited by Colowick S. P. Kaplan N. O. New York: vol I: p 138
    [Google Scholar]
  7. Hallander H. O. 1963; Fractionation of staphylococcal toxins by gel filtration. Acta, path, microbiol. scand 59:543
    [Google Scholar]
  8. Kantor H. S., Temples B., Shaw W. V. 1972; Staphylococcal delta hemolysin: purification and characterization. Archs Biochem. [Biophys.] 151:142
    [Google Scholar]
  9. Kreger A. S., Kim K. S., Zaboretsky F., Bernheimer A. W. 1971; Purification and properties of staphylococcal delta hemolysin. Infect. Immun 3:449
    [Google Scholar]
  10. Markham R. 1942; A steam distillation apparatus suitable for micro-Kjeldahl analysis. Biochem. J 36:790
    [Google Scholar]
  11. Wiseman G. M., Cairo J. D. 1967; The nature of staphylococcal beta hemolysin. I. Mode of action. Can. J. Microbiol 13:369
    [Google Scholar]
  12. Wiseman G. M., Cairo J. D. 1972; Further observations on the mode of action of the alpha toxin of Staphylococcus aureus “ Wood 46 ”. Can. J. Microbiol 18:987
    [Google Scholar]
  13. Wiseman G. M., Caird J. D., Fackrell H. B. 1975; Trypsin-mediated activation of the alpha hemolysin of Staphylococcus aureus “ Wood ” 46. J. med. Microbiol 8: in press
    [Google Scholar]
  14. Yoshida A. 1963; Staphylococcal delta hemolysin. I. Purification and chemical properties. Biochim. biophys. Acta 71:544
    [Google Scholar]
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