1887

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Volume 7, Issue 4

Immunogenicity of the δ-Haemolysin of Free

Abstract

SUMMARY

Antibody to δ-lysin of was produced in rabbits given injections of purified antigen. Removal of normal serum inhibitors was effected by ammonium-sulphate fractionation and ion-exchange chromato-graphy. The purified anti-δ-lysin showed a single line of precipitation with purified antigen in Ouchterlony agar gel-diffusion tests and when subjected to immunoelectrophoresis. The line of precipitation also coincided with the zone of haemolysis caused by δ-lysin when the agar gel-diffusion test was performed in blood-agarose plates.

A plot of the data obtained in a quantitative precipitin test performed with purified antibody and δ-lysin showed a characteristic curve confirming their interaction; in control tests with non-immune sera in place of δ-antibody, no precipitation was evident.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1974
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1974-11-01
2024-04-26
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Immunogenicity of the δ-Haemolysin of Staphylococcus Aureus
J. Med. Microbiol. 7, 411 (1974); https://doi.org/10.1099/00222615-7-4-411
/content/journal/jmm/10.1099/00222615-7-4-411
/content/journal/jmm/10.1099/00222615-7-4-411
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