A defined medium containing mineral salts, glucose and vitamins was developed in which large amounts of enterotoxin were produced by Escherichia coli strain P16 (serotype 09; K9) if the culture was well aerated during incubation for at least 48 hours and the pH was con- trolled to between 7.4 and 8.1.

After gel filtration of the supernates, the fractions with enterotoxic activity obtained from cultures in the defined medium contained considerably less ultra-violet-light absorbent material than did the corresponding fractions from cultures in “syncase” medium.

We are grateful to Mrs H. Steels, Mrs K. Martin and Mr M. Everett for their efficient technical assistance.


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