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1887

Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 7, Issue 2

A Probable Example of R-Factor Recombination in the Human Gastro-Intestinal Tract No Access

Abstract

SUMMARY

A previous study described the isolation of multiply-resistant strains of from the gastro-intestinal tract after the ingestion of a strain carrying an R factor (Rl) that confers resistance to ampicillin (A), chloram-phenicol (C), kanamycin (K), streptomycin (S) and sulphadimidine (Su). One such strain carried the R factor R174 (ACKSSuT)–which was in addition resistant to tetracycline (T)–and another carried the factor R157 (AST).

The minimum inhibitory concentrations of these antibiotics for strains, isogenic but for the R factors, were consistent with the formation of factor R174 by recombination of the AST and ACKSSu resistance determinants of the other two R factors. The factor Rl 74 determined a streptomycin-phosphorylase enzyme also present in factor R157, together with the strepto-mycin-spectinomycin resistance determinant of factor Rl, and all three R-factors shared the same type of ampicillin resistance. Factor R174 carried all the other antibiotic-resistance determinants (TCKSu) of the parent plasmids and is thus probably a recombinent of factor Rl and R157.

DNA-DNA hybridisation showed that factor R174 contained almost all of the DNA-base sequences of factors R157 and Rl. There was also approximately 50% homology between the latter two R factors which may account for the occurrence of recombination.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1975
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1974-05-01
2025-01-18
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A Probable Example of R-Factor Recombination in the Human Gastro-Intestinal Tract
J. Med. Microbiol. 7, 251 (1974); https://doi.org/10.1099/00222615-7-2-251
/content/journal/jmm/10.1099/00222615-7-2-251
/content/journal/jmm/10.1099/00222615-7-2-251
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