1887

Abstract

SUMMARY

The survival of “marked” strains of bearing antibiotic-resistance-transfer (R) factors was studied in the faeces of healthy human volunteers for 7 days after ingestion. Two R factors, determining resistance to ampicillin/chloramphenicol/kanamycin/streptomycin/sulphonamides and to chloramphenicol/streptomycin/sulphonamides/tetracyclines were used in 14 experiments on 12 persons. Strains without R factors served as controls. Both R-factor-bearing and R-factor-free strains were identified by chromosomally determined resistance markers.

The R-factor-bearing organisms were isolated from the faeces in fewer numbers than the controls in 52 out of the 54 samples studied. In the two samples in which the reverse phenomenon was observed the effect was not marked. The fall in the number of R-factor-bearing organisms was generally progressive, and 4 days after ingestion the mean faecal population of R-factor-bearing organisms was only 0.5% of the control organisms. The relative decline in the R-factor population probably resulted from impaired vitality of organisms bearing these plasmids rather than from loss of R factors from the cell.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-7-1-85
1974-02-01
2022-05-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/7/1/medmicro-7-1-85.html?itemId=/content/journal/jmm/10.1099/00222615-7-1-85&mimeType=html&fmt=ahah

References

  1. Anderson J. D. 1973; The effect of resistance-transfer (R) factor carriage upon the survival of Escherichia coli in vitro and experimentally in man. J. med. Microbiol 6:xix
    [Google Scholar]
  2. Anderson J. D., Gillespie W. A., Richmond M. H. 1973a; Chemotherapy and antibiotic resistance transfer between Enterobacteria in the human gastro-intestinal tract. J. med. Microbiol 6:461
    [Google Scholar]
  3. Anderson J. D., Ingram L. C., Richmond M. H., Wiedemann B. 1973b; Studies on the nature of plasmids arising from conjugation in the human gastro-intestinal tract. J. med. Microbiol 6:475
    [Google Scholar]
  4. Clowes R. C. 1972; Molecular structure of bacterial plasmids. Bad. Rev. 36:361
    [Google Scholar]
  5. Cowan S. T., Steel K. J. 1965; Manual for the identification of medical bacteria. London:
    [Google Scholar]
  6. Finland M. 1972; Changing patterns of susceptibility of common bacterial pathogens to antimicrobial agents. Ann. intern. Med. 76:1009
    [Google Scholar]
  7. Gillespie W. A., Lee P. A., Linton K. B., Rowland A. J. 1971; Antibiotic resistance of coliform bacilli in urinary infection acquired by women outside hospital. Lancet 2:675
    [Google Scholar]
  8. Jarolmen H., Kemp G. 1969; R-factor transfer in vivo. J. Bact 99:487
    [Google Scholar]
  9. Lowbury E. J. L., Babb J. R., Roe E. 1972; Clearance from hospital of Gram negative bacilli that transfer carbenicillin resistance to Pseudomonas aeruginosa. Lancet 2:941
    [Google Scholar]
  10. Price D. J., Sleigh J. D. 1970; Control of infection due to Klebsiella aerogenes in a neurosurgical unit by withdrawal of all antibiotics. Lancet 2:1213
    [Google Scholar]
  11. Richmond M. H., Sykes R. B. 1973; The β-lactamases of Gram-negative bacteria and their possible physiological role. Adv. microb. Physiol 9: edited by Rose A. H., Tempest D. W. London & New York: p 31
    [Google Scholar]
  12. Slocombe B., Sutherland R. 1969; Sensitivity of Gram-negative bacilli to ampicillin after six years clinical use. J. clin. Path 22:644
    [Google Scholar]
  13. Smith H. W. 1972; The effect on virulence of transferring R factors to Salmonella typhi-murium in vivo. J. med. Microbiol 5:451
    [Google Scholar]
  14. Smith H. W., Halls S. 1966; Observations on infective drug resistance in Britain. Br. med. J. 1:266
    [Google Scholar]
  15. Thiele E. H. 1970; Virulence of R-factor-bearing Salmonella typhimurium. Infect. Immun 2:516
    [Google Scholar]
  16. Walton J. R. 1972; Loss of antibiotic resistance from bacteria exposed to anthelmintic agents. Vet. Rec. 91:629
    [Google Scholar]
  17. Watanabe T. 1963; Infective heredity of multiple drug resistance in bacteria. Bact. Rev 27:87
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-7-1-85
Loading
/content/journal/jmm/10.1099/00222615-7-1-85
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error