1887

Abstract

SUMMARY

Methicillin-resistant staphylococci are characterised by: (a) chromosomal resistance to streptomycin, (b) tetracycline resistance determined by a stable plasmid, (c) production of orange pigment, (d) a similar degree of survival on glass. We consider that all or almost all isolates of methicillin-resistant staphylococci have evolved from a single clone. If so, plasmids determining a variety of antibiotic resistances (penicillinase, penicillinase/fusidic acid, erythromycin, erythromycin/lincomycin, neomycin and chloramphenicol) have probably been acquired in recent years by their transfer from other strains. One isolate (no. 11164) has probably acquired novobiocin resistance by mutation and lost the genes determining pigment and survival.

Strain no. 13136 contains a “penicillinase plasmid” with a molecular weight of about 20 million daltons and a plasmid specifying high-level resistance to tetracycline (MIC 200 μg per ml) of molecular weight about 2.9 million daltons. The latter plasmid was also present in other methicillin-resistant strains. After storage of strain no. 13136, some colonies were resistant to only low levels of tetracycline (MIC 2.5 μg per ml). In these derivatives, the “tetracycline plasmid” of molecular weight 2.9 million daltons was replaced by a plasmid of 2.2 million daltons.

Although methicillin resistance can be lost irreversibly from strains nos. 13136, 2273 and 9463, no discrete CCC-DNA component corresponding to a plasmid determining this resistance was resolved in any of them.

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1973-11-01
2022-07-02
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References

  1. Al Salihy S. M., James A. M. 1972; Loss of methicillin-resistance from resistant strains of Staphylococcus aureus. Lancet, Lond 2:331
    [Google Scholar]
  2. Annear D. I., Grubb W. B. 1973; Spontaneous loss of methicillin resistance in Staphylococcus aureus. Lancet, Lond 1:110
    [Google Scholar]
  3. Asheshov Elizabeth H. 1966; Loss of antibiotic resistance in Staphylococcus aureus resulting from growth at high temperature. J. Gen. Microbiol 42:403
    [Google Scholar]
  4. Barber Mary. 1961; Methicillin-resistant Staph. pyogenes. J. Clin. Path 14:385
    [Google Scholar]
  5. Barber Mary, Waterworth Pamela M. 1962; Antibacterial activity of the penicillins. Br. Med. J 1:1157
    [Google Scholar]
  6. Beck A., Evans I. Lynn. 1963; Resistance to the newer penicillins. Br. Med. J 1:887
    [Google Scholar]
  7. Borowski J., Kamienska K., Rutecka I. 1964; Methicillin-resistant staphylococci. Br. Med. J 1:983
    [Google Scholar]
  8. Bulow P. 1971; Staphylococci in Danish hospitals during the last decade: factors influencing some properties of predominant epidemic strains. Ann. N.Y. Acad. Sci 182:21
    [Google Scholar]
  9. Chabbert Y.-A., Baudens J. G. 1962; Souches de staphylocoques resistant naturelle-ment a la mdthicilline et l5-methyl-3-phenyl-4-iso-oxazolyl-penicilline. Ann. Inst. Pasteur, Paris 103:222
    [Google Scholar]
  10. Cohen S., Sweeney Helen M. 1970; Transduction of methicillin resistance in Staphylococcus aureus dependent on an unusual specificity of the recipient strain. J. Bact 104:1158
    [Google Scholar]
  11. Colley E. W., McNicol M. W., Bracken P. M. 1965; Methicillin-resistant staphylococci in a general hospital. Lancet, Lond 1:595
    [Google Scholar]
  12. Dornbusch Katherine, Hallander H. O., Lofquist F. 1969; Extrachromosomal control of methicillin resistance and toxin production in Staphylococcus aureus. J. Bact 98:351
    [Google Scholar]
  13. Dyke K. G. H. 1969; Penicillinase production and intrinsic resistance to penicillins in methicillin-resistant cultures of Staphylococcus aureus. J. Med. Microbiol 2:261
    [Google Scholar]
  14. Eriksen K. R., Erichsen Ingrid. 1964; Resistance to methicillin, isoxazolyl penicillins and cephalothin in Staphylococcus aureus. Acta path. microbiol. scand 62:255
    [Google Scholar]
  15. Garrod L. P., O’Grady F. 1971 Antibiotic and chemotherapy 3rd ed Edinburgh and London: p 265
    [Google Scholar]
  16. Grinsted J., Lacey R. W. 1973a; Genetic variation of streptomycin-resistance in clinical strains of Staphyloccus aureus. J. Med. Microbiol 6:351
    [Google Scholar]
  17. Grinsted J., Lacey R. W. 1973b; Ecological and genetic implications of pigmentation in Staphylococcus aureus. J. Gen. Microbiol 75:259
    [Google Scholar]
  18. Grubb W. B., Annear D. L. 1972; Spontaneous loss of methicillin resistance in Staphylococcus aureus at room temperature. Lancet, Lond 2:1257
    [Google Scholar]
  19. Harding J. W. 1963; Infections due to methicillin-resistant strains of Staphylococcus pyogenes. J. Clin. Path 16:268
    [Google Scholar]
  20. Harwood J. H., Smith D. H. 1969; Resistance factor-mediated streptomycin resistance. J. Bact 97:1262
    [Google Scholar]
  21. Jacobs S. I., Willis A. T. 1964; Some physiological characteristics of neomycin and kanamycin-resistant strains of Staphylococcus aureus. J. Clin. Path 17:612
    [Google Scholar]
  22. Jessen O., Rosendal Kirsten Bulow P., Faber V., Eriksen K. R. 1969; Changing staphylococci and staphylococcal infections. A ten-year study of bacteria and cases of bacteraemia. New Engl. J. Med 281:627
    [Google Scholar]
  23. Jevons M. Patricia, Coe A. W., Parker M. T. 1963; Methicillin resistance in staphylococci. Lancet, Lond 1:904
    [Google Scholar]
  24. Jevons M. Patricia, John Madeleine, Parker M. T. 1966; Cultural characters of a newly recognised group of hospital staphylococci. J. Clin. Path 19:305
    [Google Scholar]
  25. Jevons M. Patricia, Parker M. T. 1964; The evolution of new hospital strains of Staphylococcus aureus. J. Clin. Path 17:243
    [Google Scholar]
  26. Kayser F. H., Felix M., Wust J. 1972; Extrachromosomal and chromosomal drug resistance in methicillin resistant Staphylococcus aureus. In Bacterial plasmids and antibiotic resistance edited by Krdmery V. Rosival L., Watanabe T. New York: p 371
    [Google Scholar]
  27. Kayser F. H., Wust J., Corrodi P. 1972; Transduction and elimination of resistance determinants in methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother 2:217
    [Google Scholar]
  28. Lacey R. W. 1971; High-frequency transfer of neomycin resistance between naturally occurring strains of Staphylococcus aureus. J. Med. Microbiol 4:73
    [Google Scholar]
  29. Lacey R. W. 1972a; Genetic control in methicillin-resistant strains of Staphylococcus aureus. J. Med. Microbiol 5:497
    [Google Scholar]
  30. Lacey R. W. 1972b; Effect of antibiotic resistance on the survival of Staphylococcus aureus. J. Clin. Path 25:713
    [Google Scholar]
  31. Lacey R. W. 1972c; Transfer of chromosomal genes between staphylococci in mixed cultures. J. Gen. Microbiol 71:399
    [Google Scholar]
  32. Lacey R. W., Chopra I. 1972; Evidence for mutation to streptomycin resistance in clinical strains of Staphylococcus aureus. J. Gen. Microbiol 73:175
    [Google Scholar]
  33. Lacey R. W., Grinsted J. 1972; Linkage of fusidic acid resistance to the penicillinase plasmid in Staphylococcus aureus. J. Gen. Microbiol 73:501
    [Google Scholar]
  34. Lang D., Mttani M. 1970; Simplified quantitative electron microscopy of biopolymers. Biopolymers 9:373
    [Google Scholar]
  35. Novick R. P., Bouanchaud D. 1971; Extrachromosomal nature of drug resistance in Staphylococcus aureus. Ann. N.Y. Acad. Sci 182:279
    [Google Scholar]
  36. Parker M. T., Hewitt J. H. 1970; Methicillin resistance in Staphylococcus aureus. Lancet, Lond 1:800
    [Google Scholar]
  37. Smith Katherine, Novick R. P. 1972; Genetic studies on plasmid-linked cadmium resistance in Staphylococcus aureus. J. Bact 112:761
    [Google Scholar]
  38. Stewart G. T., Holt R. J. 1963; Evolution of natural resistance to the new penicillins. Br. Med. J 1:308
    [Google Scholar]
  39. Tseng J. T., Bryan L. E., van den Eizen H. M. 1972; Mechanisms and spectrum of streptomycin resistance in a natural population of Pseudomonas aeruginosa. Antimicrob. Agents Chemother 2:136
    [Google Scholar]
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