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Extraction with 0.4 per cent. rivanol, at pH 9.0, provides a rapid and economical method of removing non-specific inhibitors of rubella virus HA from human serum without loss of specific antibody; natural agglutinins for pigeon erythrocytes are largely removed. Serum HI antibody titres are comparable to those obtained with the dextran sulphate-CaCl2 extraction method.
Possible sources of error in the HI test due to the method of serum extraction are discussed.
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