Of the total amount of staphylococcal a-toxin produced in batch cultures, 1 per cent. or less appeared to be cell-associated, indicating rapid release of the toxin into the environment. The level of cell-associated haemolysin reached its maximum at the onset of the stationary phase of growth and decreased thereafter. Cell-associated haemolysin was isolated by the combined use of gel filtration and iso-electric focusing and was characterised by several criteria. Purified material closely resembled the extracellular form of staphylococcal a-toxin.

The authors gratefully acknowledge the photographic assistance of Mr Ian McKee. This work was supported in part by grants from The Royal Society and the Scottish Home and Health Department.


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