1887

Abstract

Summary

Electrofocusing resolved six distinct haemolytic components in crude ammonium sulphate-precipitated staphylococcal a-toxin. Four of these had the properties of staphylococcal a-toxin as judged by several criteria and were designated a (I = 8·55±0·12); a (I = 9·15±0·07); a (I = 7·36±0·03); a (I = 6·28±0·11); 85·95 per cent. of the recovered activity was found as the a component. Reversible interconversion of a and a was demonstrated and these forms showed similar patterns in disk-gel electrophoresis; each showed a main band having a molecular weight of 36,000 and a second band having a molecular weight of 170,000. In the presence of 6M urea a yielded a single haemolytic component having a I of 8·50, and electrophoresis of a and a in urea revealed a single main band.

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/content/journal/jmm/10.1099/00222615-5-1-113
1972-02-01
2024-12-10
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