1887

Abstract

Summary

Electrofocusing resolved six distinct haemolytic components in crude ammonium sulphate-precipitated staphylococcal a-toxin. Four of these had the properties of staphylococcal a-toxin as judged by several criteria and were designated a (I = 8·55±0·12); a (I = 9·15±0·07); a (I = 7·36±0·03); a (I = 6·28±0·11); 85·95 per cent. of the recovered activity was found as the a component. Reversible interconversion of a and a was demonstrated and these forms showed similar patterns in disk-gel electrophoresis; each showed a main band having a molecular weight of 36,000 and a second band having a molecular weight of 170,000. In the presence of 6M urea a yielded a single haemolytic component having a I of 8·50, and electrophoresis of a and a in urea revealed a single main band.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-5-1-113
1972-02-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/5/1/medmicro-5-1-113.html?itemId=/content/journal/jmm/10.1099/00222615-5-1-113&mimeType=html&fmt=ahah

References

  1. Arbuthnott J. P., Freer J. H., Bernheimer A. W. 1967; Physical states of staphylococcal α-toxin. J Bact 94:1170
    [Google Scholar]
  2. Bernheimer A. W. 1970 In Microbial toxins. ed. by Ajl S. J., Kadis S., Montie T. C. New York 1:183
    [Google Scholar]
  3. Bernheimer A. W., Grushoff Phyllis, Avigad Lois S. 1968; Isoelectric analysis of cytolytic bacterial proteins. J Bact 95:2439
    [Google Scholar]
  4. Bernheimer A. W., Schwartz Lois L. 1963; Isolation and composition of staphylococcal alpha toxin. J Gen Microbiol 30:455
    [Google Scholar]
  5. Davis B. J. 1964; Disc electrophoresis II. Method and application to human serum proteins. Ann N Y Acad Sci 121:404
    [Google Scholar]
  6. De Vito E., SantomÉ J. A. 1966; Disc electrophoresis of proteins in the presence of sodium dodecyl sulphate. Experientia 22:124
    [Google Scholar]
  7. Freer J. H., Arbuthnott J. P., Bernheimer A. W. 1968; Interaction of staphylococcal α-toxin with artificial and natural membranes. J Bact 95:1153
    [Google Scholar]
  8. Kreger A. S., Kim K., Zaboretzky F., Bernheimer A. W. 1971; Purification and properties of staphylococcal delta haemolysin. Infection Immun 3:449
    [Google Scholar]
  9. Lowry O. H., Rosebrough Nira J., Farr A. L., Randall Rose J. 1951; Protein measurement with the Folin phenol reagent. J Biol Chem 193:265
    [Google Scholar]
  10. MÖllby R., Wadstrom T. 1970 Studies on haemolysins from Staphylococcus aureus by the method of isoelectric focusing . In Protides of the biological fluids. Proceedings of the 17th colloquium, Bruges, 1969, ed. by Peeters H. Oxford:465
    [Google Scholar]
  11. Neville D. M. Jr 1967; Fractionation of cell membrane protein by disc electrophoresis.. Biochim biophys Acta 133:168
    [Google Scholar]
  12. Reynolds Jacqueline A., Tanford C. 1970; Binding of dodecyl sulfate to proteins at high binding ratios. Possible implications for the state of proteins in biological membranes. Proc Natn Acad Sci USA 66:1002
    [Google Scholar]
  13. Shapiro A. L., Vinuela E., Maizel J. V. Jr 1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun 28:815
    [Google Scholar]
  14. Smyth C. J., Arbuthnott J. P. 1969; Isoelectric focusing of Clostridium perfringens α- and θ-toxins. J Gen Microbiol 59: Pv
    [Google Scholar]
  15. Sugahara T., Ohsaka A. 1970; Two molecular forms of Clostridium perfringens α-toxin associated with lethal, hemolytic and enzymatic activities. Jap J Med Sci Biol 23:61
    [Google Scholar]
  16. Wadstrom T. 1968; Studies on extracellular proteins from Staphylococcus aureus. IV. Separation of α-toxin by isoelectric focusing. Biochim biophys Acta 168:228
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-5-1-113
Loading
/content/journal/jmm/10.1099/00222615-5-1-113
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error