1887

Abstract

The copy number and genetic location of IS have provided evidence of strain relatedness in many serotypes of . In this study, 100 isolates of the related serotypes Livingstone (6,7:d:l, w) and Eimsbuettel (6,7,14:d:l, w), representing 10 ribotype/biotype (RT/BT) groups isolated from human and non-human sources in seven countries over a 26-year period, were examined for their IS profiles. The distribution of IS in strains of these serotypes was limited, being present in all 53 isolates of ribotype 1 (RT1) and its variant type RT6, in one of five isolates of RT5 but in none of 42 isolates of RTs 2, 3 or 4. Although the seven IS profiles identified in RT1 isolates were of little value for further discrimination within different biotype groups, they were extremely valuable for confirming serotype: isolates of RT1/BT8/IS profile A (or its variants) and those of RT1/BT3/IS profile B (or its variants) were almost invariably associated with serotypes Livingstone and Eimsbuettel, respectively.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-47-9-791
1998-09-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/47/9/medmicro-47-9-791.html?itemId=/content/journal/jmm/10.1099/00222615-47-9-791&mimeType=html&fmt=ahah

References

  1. Lam S., Roth J. R. IS200: a Salmonella-specific insertion sequence. Cell 1983; 34:951–960
    [Google Scholar]
  2. Gibert I., Barbé J., Casadesus J. Distribution of insertion sequence IS200 in Salmonella and Shigella. J Gen Microbiol 1990; 136:2555–2560
    [Google Scholar]
  3. Gibert I., Carroll K., Hillyard D. R., Barbé J., Casadesus J. IS200 is not a member of the IS600 family of insertion sequences. Nucleic Acids Res 1991; 19:13–43
    [Google Scholar]
  4. Threlfall E. J., Powell N. G., Rowe B. Differentiation of salmonellas by molecular methods. PHLS Microbiology’ Digest 1994; 11:199–202
    [Google Scholar]
  5. Stanley J., Saunders N. DNA insertion sequences and the molecular epidemiology of Salmonella and Mycobacterium. J Med Microbiol 1996; 45:236–251
    [Google Scholar]
  6. McAllister T. A., Roud J. A., Marshall A., Flolland B. M., Turner T. L. Outbreak of Salmonella eimsbuettel in newborn infants spread by rectal thermometers. Lancet 1986; 1:1262–1264
    [Google Scholar]
  7. Old D. C., Porter-Boveri M., Munro D. S. Human infection in Tayside, Scotland due to Salmonella serotype Livingstone. J Med Microbiol 1994; 40:134–140
    [Google Scholar]
  8. Crichton P. B., Old D. C., Taylor A., Rankin S. C. Characterisation of strains of Salmonella serotype Livingstone by multiple typing. J Med Microbiol 1996; 44:325–331
    [Google Scholar]
  9. Old D. C., Crichton P. B., Taylor A., Rankin S. C. Phenotypic and genotypic discrimination of strains of Salmonella serotype Eimsbuettel from human and animal sources. J Med Microbiol 1997; 46:617–622
    [Google Scholar]
  10. Wilson K. Preparation of genomic DNA from bacteria. In Ausubel F. M., Brent R., Kingston R. E. (eds) Current protocols in molecular biology 1987–1988 vol 1 New York: Wiley; 19872.4.1–2.4.5
    [Google Scholar]
  11. Miller J. H. Experiments in molecular genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 1972433
    [Google Scholar]
  12. Stanley J., Bumens A., Powell N., Chowdry N., Jones C. The insertion sequence IS200 fingerprints chromosomal genotypes and epidemiological relationships in Salmonella heidelberg. J Gen Microbiol 1992; 138:2329–2336
    [Google Scholar]
  13. Sambrook J., Fritsch E. F., Maniatis T. Molecular cloning: a laboratory manual. 2nd edn, vol 3 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 1989 B.13
    [Google Scholar]
  14. Stanley J., Baquar N., Bumens A. Molecular subtyping scheme for Salmonella panama. J Clin Microbiol 1995; 33:1206–1211
    [Google Scholar]
  15. Le Minor L. Conversions antigéniques chez les Salmonella. VII. Acquisition du facteur 14 par les Salmonella du sous-groupe C1 (6,7), aprés lysogénisation par un phage tempéré isolé de cultures du sous-groupe C4 [6, (7), (14)]. Ann Inst Pasteur 1965; 109:505–515
    [Google Scholar]
  16. Popoff M. Y., Le Minor L. [Antigenic formulas of the Salmonella serovars, 6th revision.]. WHO Collaborating Center for Reference and Research on Salmonella Paris: Institut Pasteur; 1992
    [Google Scholar]
  17. Anon Salmonella serotypes recorded in the PHLS Salmonella data sets. Communicable Disease Report CDR Wkly 1997; 7:338
    [Google Scholar]
  18. Ezquerra E., Bumens A. P., Frith K., Costas M., Stanley J. Molecular genotype analysis of Salmonella bovismorbificans. Mol Cell Probes 1993; 7:45–54
    [Google Scholar]
  19. Torre E., Threlfall E. J., Hampton M. D., Ward L. R., Gibert I., Rowe B. Characterization of Salmonella virchow phage types by plasmid profile and IS200 distribution. J Appl Bacteriol 1993; 75:435–440
    [Google Scholar]
  20. Pelkonen S., Romppanen E.-L., Siitonen A., Pelkonen J. Differentiation of Salmonella serovar Infantis isolates from human and animal sources by fingerprinting IS200 and 16S rrn loci. J Clin Microbiol 1994; 32:2128–2133
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-47-9-791
Loading
/content/journal/jmm/10.1099/00222615-47-9-791
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error