1887

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Volume 47, Issue 7

Comparison of two serological methods and a polymerase chain reaction-enzyme immunoassay for the diagnosis of acute respiratory infections with in adults Free

Abstract

is a common respiratory tract pathogen. Serological methods currently used for the diagnosis of infection lack specificity, give ambiguous results from a single serum sample and often provide only a retrospective diagnosis. A prospective study was undertaken to assess whether PCR could be a useful addition to the serological techniques routinely practised for diagnosis. This study investigated 68 adult patients with a diagnosis of acute respiratory infection. Acute and convalescent serological determination of antibodies to were performed by means of an rELISA test and a micro-immunofluorescence (MIF) test. Nasopharyngeal aspirates or bronchoalveolar lavage specimens and bronchial aspirates obtained from the 68 patients were evaluated by PCR-enzyme immunoassay (PCR-EIA) for the presence of and by immunofluorescence assay and cell culture for virus identification. serology was also performed. Eight patients (11.8%) were positive by either rELISA or PCR-EIA, or both, with an infection rate of 5 (18.5%) of 27 in patients with community-acquired pneumonia, 2 (9%) of 22 in asthmatic patients and 1 (5%) of 19 in patients with an exacerbation of chronic obstructive pulmonary disease. Serological evidence of acute infection was found in four of these patients with the rELISA test and in three others with the MIF test. PCR-EIA detected DNA in four specimens, but there were concordant results with both rELISA and PCR-EIA in only one patient. A positive PCR-EIA was also obtained in a patient who did not show an antibody response in acute serum. The discrepancy between serological and PCR-EIA results reflects the difficulties in routine laboratory diagnosis of infection and the necessity for further studies with optimised techniques.

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© 1998 The Pathological Society of Great Britain and Ireland
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1998-07-01
2024-04-26
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Comparison of two serological methods and a polymerase chain reaction-enzyme immunoassay for the diagnosis of acute respiratory infections with Chlamydia pneumoniae in adults
J. Med. Microbiol. 47, 615 (1998); https://doi.org/10.1099/00222615-47-7-615
/content/journal/jmm/10.1099/00222615-47-7-615
/content/journal/jmm/10.1099/00222615-47-7-615
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