1887

Abstract

SUMMARY

Whole bacterial cells of as well as purified staphylococcal peptidoglycan (PG) have been demonstrated to stimulate human monocytes to release cytokines. Hypothesising that the phenomenon of changes induced by β-lactam antibiotics in cell-wall composition may alter the immunological properties of the intact cell wall as well as of purified cell-wall components, this study assessed whether cytokine release by human monocytes is altered if cells from strains grown in the presence or absence of sub-minimal inhibitory concentrations of oxacillin are used as stimuli. Whole bacterial cells and isolated PG from these strains, grown in the presence of oxacillin, showed a significantly reduced stimulation of tumour necrosis factor-α, interleukin (IL)-1β and IL-6 release by human monocytes in a concentration-dependent fashion. The serum-induced potentiation of cytokine production by human monocytes in response to PG with modified cross-linking was also reduced. These observations may have particular relevance for staphylococcal infections, in which clinically achievable β-lactam concentrations do not suppress staphylococcal growth yet may alter and possibly enhance virulence.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-47-6-533
1998-06-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/47/6/medmicro-47-6-533.html?itemId=/content/journal/jmm/10.1099/00222615-47-6-533&mimeType=html&fmt=ahah

References

  1. Mattsson E., Verhage L., Rollof J., Fleer A., Verhoef J., van Dijk H. Peptidoglycan and teichoic acid from Staphylococcus epidermidis stimulate human monocytes to release tumor necrosis factor-α, interleukin-1β and interleukin-6. FEMS Immunol Med Microbiol 1993; 7:281–287
    [Google Scholar]
  2. Timmerman C. P., Mattsson E., Martinez-Martinez L. Induction of tumor necrosis factor from human monocytes by staphylococci and staphylococcal peptidoglycans. Infect Immun 1993; 61:4167–4172
    [Google Scholar]
  3. Vacheron F., Guenounou M., Nauciel C. Adjuvant active peptidoglycans induce the secretion of a cytotoxic factor by macrophages. In Seidl P. H., Schleifer K. H. (eds) Biological properties of peptidoglycan New York: W de Gruyter and Co; 1986395–400
    [Google Scholar]
  4. Wakabayashi G., Gelfand J. A., Jung W. K., Connolly R. J., Burke J. F., Dinarello C. A. Staphylococcus epidermidis induces complement activation, tumor necrosis factor and interleukin-1, a shock-like state and tissue injury in rabbits without endotoxemia. Comparison to Escherichia coli. J Clin Invest 1991; 87:1925–1935
    [Google Scholar]
  5. Mattsson E., Rollof J., Verhoef J., Van Dijk H., Fleer A. Serum-induced potentiation of tumor necrosis factor alpha production by human monocytes in response to staphylococcal peptidoglycan: involvement of different serum factors. Infect Immun 1994; 62:3837–3843
    [Google Scholar]
  6. Heumann D., Barras C., Severin A., Glauser M. P., Tomasz A. Gram-positive cell walls stimulate synthesis of tumor necrosis factor alpha and interleukin-6 by human monocytes. Infect Immun 1994; 62:2715–2721
    [Google Scholar]
  7. De Kimpe S. J., Kengatharan M., Thiemermann C., Vane J. R. The cell wall components peptidoglycan and lipoteichoic acid from Staphylococcus aureus act in synergy to cause shock and multiple organ failure. Proc Natl Acad Sci USA 1995; 92:10359–10363
    [Google Scholar]
  8. Martinez-Martinez L., Timmermann C. P., Fleer A., Verhoef J. Chemiluminescence of human polymorphonuclear leukocytes after stimulation with whole cells and cell-wall components of Staphylococcus epidermidis. J Med Microbiol 1993; 39:196–203
    [Google Scholar]
  9. Wilkinson B. J. Biology. In Crossley K. B., Archer G. L. (eds) The staphylococci in human disease New York: Churchill Livingstone; 19971–38
    [Google Scholar]
  10. Zeiger A. R., Wong W., Chatterjee A. N., Young F. E., Tuazon C. U. Evidence for the secretion of soluble peptidoglycans by clinical isolates of Staphylococcus aureus. Infect Immun 1982; 37:1112–1118
    [Google Scholar]
  11. Gold M. R., Miller C. L., Mishell R. I. Soluble non-cross-linked peptidoglycan polymers stimulate monocyte-macrophage inflammatory functions. Infect Immun 1985; 49:731–741
    [Google Scholar]
  12. De Jonge B. L. M., Chang Y.-S., Gage D., Tomasz A. Peptidoglycan composition of a highly methicillin-resistant Staphylococcus aureus strain. J Biol Chem 1992; 267:11248–11254
    [Google Scholar]
  13. De Jonge B. L. M., Chang Y.-S., Gage D., Tomasz A. Peptidoglycan composition in heterogeneous Tn551 mutants of a methicillin-resistant Staphylococcus aureus strain. J Biol Chem 1992; 267:11255–11259
    [Google Scholar]
  14. De Jonge B. L. M., Tomasz A. Abnormal peptidoglycan produced in a methicillin-resistant strain of Staphylococcus aureus grown in the presence of methicillin: functional role for penicillin-binding protein 2A in cell wall synthesis. Antimicrob Agents Chemother 1993; 37:342–346
    [Google Scholar]
  15. Snowden M. A., Perkins H. R. Cross-linking and O-acetylation of peptidoglycan in Staphylococcus aureus (strains H and MR-1) grown in the presence of sub-growth-inhibitory concentration of β-lactam antibiotics. J Gen Microbiol 1991; 137:1661–1666
    [Google Scholar]
  16. Peterson P. K., Wilkinson B. J., Kim Y. The key role of peptidoglycan in the opsonization of Staphylococcus aureus. J Clin Invest 1978; 61:597–609
    [Google Scholar]
  17. Wilkinson B. J., Kim Y., Peterson P. K., Quie P. G., Michael A. F. Activation of complement by cell surface components of Staphylococcus aureus. Infect Immun 1978; 20:388–392
    [Google Scholar]
  18. Bartlett G. R. Phosphorus assay in column chromatography. J Biol Chem 1959; 234:466–468
    [Google Scholar]
  19. Neoh S. H., Gordon T. P., Roberts-Thomson P. J. A simple one-step procedure for preparation of Cl-deficient human serum. J Immunol Methods 1984; 69:277–280
    [Google Scholar]
  20. Jessen T. E., Barkholt Y., Welinder K. G. A simple alternative pathway for hemolytic assay of human complement component C3 using methylamine-treated plasma. J Immunol Methods 1983; 60:89–100
    [Google Scholar]
  21. Boyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of mononuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl 1968; 97:77–89
    [Google Scholar]
  22. Lorian Y., Gemmell C. G. Effect of low antibiotic concentrations on bacteria: effects on ultrastructure, virulence, and susceptibility to immunodefenses. In Lorian V. (ed) Antibiotics in laboratory medicine 3rd edn Baltimore: Williams and Wilkins; 1991493–555
    [Google Scholar]
  23. Kemodle D. S., McGraw P. A., Barg N. L., Menzies B. E., Voladri R. K. R., Harshman S. Growth of Staphylococcus aureus with nafcillin in vitro induces α-toxin production and increases the lethal activity of sterile broth filtrates in a murine model. J Infect Dis 1995; 172:410–419
    [Google Scholar]
  24. Mattsson E., Van Dijk H., Van Kessel K., Verhoef J., Fleer A., Rollof J. Intracellular pathways involved in tumor necrosis factor-alpha release by human monocytes on stimulation with lipopolysaccharide or staphylococcal peptidoglycan are partly similar. J Infect Dis 1996; 173:212–218
    [Google Scholar]
  25. Verbrugh H. A., van Dijk W. C., Peters R. Opsonic recognition of staphylococci mediated by cell wall peptidoglycan: antibody-independent activation of human complement and opsonic activity of peptidoglycan antibodies. J Immunol 1980; 124:1167–1173
    [Google Scholar]
  26. Verbrugh H. A., van Dijk W. C., Peters R., Van Der Tol M. E., Verhoef J. The role of Staphylococcus aureus cell-wall peptidoglycan, teichoic acid and protein A in the processes of complement activation and opsonization. Immunology 1979; 37:615–621
    [Google Scholar]
  27. Barton P. A., Warren J. S. Complement component C5 modulates the systemic tumor necrosis factor response in murine endotoxic shock. Infect Immun 1993; 61:1474–1481
    [Google Scholar]
  28. Spika J. S., Peterson P. K., Wilkinson B. J. Role of peptidoglycan from Staphylococcus aureus in leukopenia, thrombocytopenia, and complement activation associated with bacteremia. J Infect Dis 1982; 146:227–234
    [Google Scholar]
  29. Takahashi I., Kotani S., Takada H., Shiba T., Kusumoto S. Structural requirements of endotoxic lipopolysaccharides and bacteria cell walls in induction of interleukin-1. Blood Purif 1988; 6:188–206
    [Google Scholar]
  30. Ahmed A. J., Kruse J. A., Haupt M. T., Chandrasekar P. H., Carlson R. W. Hemodynamic responses to gram-positive versus gramnegative sepsis in critically ill patients with and without circulatory shock. Crit Care Med 1991; 19:1520–1525
    [Google Scholar]
  31. Nakane A., Mingawa T., Kato K. Endogenous tumor necrosis factor (cachectin) is essential to host resistance against Listeria monocytogenes infection. Infect Immun 1988; 56:2563–2569
    [Google Scholar]
  32. Mattsson E., Van Dijk H., Verhoef J., Norrby R., Rollof J. Supernatants from Staphylococcus epidermidis grown in the presence of different antibiotics induce differential release of tumor necrosis factor alpha from human monocytes. Infect Immun 1996; 64:4351–4355
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-47-6-533
Loading
/content/journal/jmm/10.1099/00222615-47-6-533
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error