1887

Abstract

A competitive polymerase chain reaction (cPCR) assay for the quantitative evaluation of growth was developed based on co-amplification of genomic DNA and a modified DNA fragment derived from a well-conserved region of the 16S rRNA gene. There was a good correlation between the number of DNA copies in the sample, indicated by competitive PCR, and the number of colony forming units determined by conventional culture methods.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-47-10-933
1998-10-01
2022-12-04
Loading full text...

Full text loading...

/deliver/fulltext/jmm/47/10/medmicro-47-10-933.html?itemId=/content/journal/jmm/10.1099/00222615-47-10-933&mimeType=html&fmt=ahah

References

  1. Chevrier D., Rasmussen S. R., Guesdon J. L. PCR product quantification by non-radioactive hybridization procedures using an oligonucleotide covalently bound to microwells. Mol Cell Probes 1993; 7:187–197
    [Google Scholar]
  2. Kolk A. H. J., Noordhoek G. T., De Leeuw O., Kuijper S., van Embden J. D. A. Mycobacterium smegmatis strain for detection of Myco bacterium tuberculosis by PCR used as internal control for inhibition of amplification and for quantification of bacteria. J Clin Microbiol 1994; 32:1354–1356
    [Google Scholar]
  3. An Q., Buxton D., Hendricks A. Comparison of amplified Q beta replicase and PCR assays for detection of Mycobacterium tuberculosis. J Clin Microbiol 1995; 33:860–867
    [Google Scholar]
  4. Boddinghaus B., Rogall T., Flohr T., Blocker H., Böttger E. C. Detection and identification of mycobacteria by amplification of rRNA. J Clin Microbiol 1990; 28:1751–1759
    [Google Scholar]
  5. Edwards U., Rogall T., Blocker H., Emde M., Böttger E. C. Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 1989; 17:7843–7852
    [Google Scholar]
  6. Kempsell K. E., Ji Y., Estrada G. I. C. E., Colston M. J., Cox R. A. The nucleotide sequence of the promoter, 16S rRNA and spacer region of the ribosomal RNA operon of Mycobacterium tuberculosis and comparison with Mycobacterium leprae precursor rRNA. J Gen Microbiol 1992; 138:1717–1727
    [Google Scholar]
  7. Nolte F. S., Metchock B. Mycobacterium. In Murray P. R., Baron E. J., Pfaller M. A., Tenover F. C., Yolken R. H. (eds) Manual of clinical microbiology 6th edn Washington, DC: American Society for Microbiology; 1997400–437
    [Google Scholar]
  8. van de Lest C. H. A., Veerkamp J. H., van Kuppevelt T. H. Image-Calc: a MicrosoftR Windows™ application for quantitative image analysis and comparison. Biotechniques 1995; 18:1050–1055
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-47-10-933
Loading
/content/journal/jmm/10.1099/00222615-47-10-933
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error