1887

Abstract

strains associated with diarrhoeal disease have been classified into several types according to the pathogenic mechanism. Among these, enteroaggregative strains (EAggEC) have been associated with persistent childhood diarrhoea. Some strains of EAggEC produce a heat-stable toxin (EAST-1) that differs from others described previously. The main goal of this case-control study was to determine the prevalence of EAggEC and EAST-1-producing strains as a cause of diarrhoea in children in Spain and to study their in-vitro susceptibility to 21 antimicrobial agents. In the case group (115 children) 22 (19%) isolates and four (3.5%) isolates were EAST-1-producing and EAggEC, respectively, whereas in the control group (79 children) four (5%) isolates produced EAST-1 (p = 0.005) and three (3.8%) isolates were EAggEC. The present study suggests that EAST-1-producing strains are associated with diarrhoeal diseases in Spanish children, whereas EAggEC strains are not. Moreover, EAST-1-producing strains showed a high susceptibility to all the antimicrobial agents tested except for ampicillin.

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1998-10-01
2022-05-28
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References

  1. Hosalot A., Corretger J., Falcó M., Rios M., Lambruschini N. Gastroenteritis aguda. Estudio prospectivo en niños de 1 a 24 meses. Arch Ped 1995; 46:268–273
    [Google Scholar]
  2. Savarino S. J. Diarrhoeal disease: current concepts and future challenges Enteroadherent Escherichia coli: a heterogeneous group of E. coli implicated as diarrhoeal pathogens. Trans R Soc Trop Med Hyg 1993; 87: (Suppl 3) 49–53
    [Google Scholar]
  3. Nataro J. P., Kaper J. B., Robins-Browne R., Pardo V., Vial P., Levine M. M. Patterns of adherence of diarrheagenic Escherichia coli to HEp-2 cells. Pediatr Infect Dis J 1987; 6:829–831
    [Google Scholar]
  4. Savarino S. J., Fasano A., Robertson D. C., Levine M. M. Entero-aggregative Escherichia coli elaborate a heat-stable enterotoxin demonstrable in an in vitro rabbit intestinal model. J Clin Invest 1991; 87:1450–1455
    [Google Scholar]
  5. Chan K. N., Phillips A. D., Knutton S., Smith H. R., Walker-Smith J. A. Enteroaggregative Escherichia coli: another cause of acute and chronic diarrhoea in England?. J Pediatr Gastroenterol Nutr 1994; 18:87–91
    [Google Scholar]
  6. Forestier C., Meyer M., Fabre-Bonte S. Enteroadherent Escherichia coli and diarrhea in children: a prospective case-control study. J Clin Microbiol 1996; 34:2897–2903
    [Google Scholar]
  7. Savarino S. J., Fasano A., Watson J. Enteroaggregative Escherichia coli heat-stable enterotoxin 1 represents another subfamily of E. coli heat-stable toxin. Proc Natl Acad Sci USA 1993; 90:3093–3097
    [Google Scholar]
  8. Savarino S. J., McVeigh A., Watson J. Enteroaggregative Escherichia coli heat-stable enterotoxin is not restricted to enteroaggregative E. coli. J Infect Dis 1996; 173:1019–1022
    [Google Scholar]
  9. Murray P. R., Baron E. J., Pfaller M. A., Tenover F. C., Yolken R. H. (eds) Manual of clinical microbiology 6th edn Washington, DC: American Society for Microbiology; 1995
    [Google Scholar]
  10. Schmidt H., Knop C., Franke S., Aleksic S., Heesemann J., Karch H. Development of PCR for screening of enteroaggregative Escherichia coli. J Clin Microbiol 1995; 33:701–705
    [Google Scholar]
  11. Vial P. A., Robins-Browne R., Lior H. Characterization of enteroadherent-aggregative Escherichia coli, a putative agent of diarrheal disease. J Infect Dis 1988; 158:70–79
    [Google Scholar]
  12. Echeverria P., Serichantalerg O., Changchawalit S. Tissue culture-adherent Escherichia coli in infantile diarrhea. J Infect Dis 1992; 165:141–143
    [Google Scholar]
  13. Bhan M. K., Raj P., Levine M. M. Enteroaggregative Escherichia coli associated with persistent diarrhea in a cohort of rural children in India. J Infect Dis 1989; 159:1061–1064
    [Google Scholar]
  14. Cravioto A., Tello A., Navarro A. Association of Escherichia coli HEp-2 adherence patterns with type and duration of diarrhoea. Lancet 1991; 337:262–264
    [Google Scholar]
  15. Gomes T. A. T., Blake P. A., Trabulsi L. R. Prevalence of Escherichia coli strains with localized, diffuse, and aggregative adherence to HeLa cells in infants with diarrhea and matched controls. J Clin Microbiol 1989; 27:266–269
    [Google Scholar]
  16. Baqui A. H., Sack R. B., Black R. E. Enteropathogens associated with acute and persistent diarrhea in Bangladeshi children < 5 years of age. J Infect Dis 1992; 166:792–796
    [Google Scholar]
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