1887

Abstract

A new selective medium (CBU agar) was compared with blood agar (BA) medium for primary isolation of from urine and skin samples of hospitalised patients. Overall, the CBU agar detected in 14 (4.6%) of 302 urine samples and the BA medium detected the organism in four (1.3%), but most cultures which were positive only on CBU agar had < 10 cfu/ml. Six strains of were isolated from 60 skin samples with CBU agar, whereas none was detected with BA. Although most skin samples had heavy inocula, the selective agar facilitated the recognition of low colony counts (≤ 10 cfu/plate) of by reducing the growth of competing flora. Challenge of the selective medium with reference and clinical strains showed that CBU agar was inhibitory for gram-negative bacteria and reduced the gram-positive flora, allowing the growth of strains. The new selective medium appears to be a useful epidemiological tool to study urinary and skin colonisation by .

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1998-01-01
2022-08-11
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References

  1. Aguado J. M., Salto E., Morales J. M. Corynebacterium urealyticum. a new and threatening pathogen for the renal transplant patient. Transplant Proc 1993; 25:1493–1494
    [Google Scholar]
  2. Coyle M. B., Lipsky B. A. Coryneform bacteria in infectious diseases: clinical and laboratory aspects. Clin Microbiol Rev 1990; 3:227–246
    [Google Scholar]
  3. Soriano F., Femandez-Roblas R. Infections caused by antibiotic-resistant Corynebacterium group D2. Eur J Clin Microbiol Infect Dis 1988; 7:337–341
    [Google Scholar]
  4. Soriano F., Aguado J. M., Ponte M. C., Femandez-Roblas R., Rodriguez-Tudela J. L. Urinary tract infection caused by Corynebacterium group D2: report of 82 cases and review. Rev Infect Dis 1990; 12:1019–1034
    [Google Scholar]
  5. Soriano F., Ponte C., Ruiz P., Zapardiel J. Non-urinary tract infections caused by multiply antibiotic-resistant Corynebacterium urealyticum. Clin Infect Dis 1993; 17:890–891
    [Google Scholar]
  6. Wood C. A., Pepe R. Bacteremia in a patient with non-urinary tract infection due to Corynebacterium urealyticum. Clin Infect Dis 1994; 19:367–368
    [Google Scholar]
  7. Zapardiel J., Nieto E., Soriano F. Urinary tract infections by β-lactam-sensitive Corynebacterium urealyticum strains. Eur J Clin Microbiol Infect Dis 1997; 16:174–176
    [Google Scholar]
  8. De Briel D., Lang J. C., Rougeron G., Chabot P., Le Faou A. Multiresistant corynebacteria in bacteriuria: a comparative study of the role of Corynebacterium group D2 and Corynebacterium jeikeium. J Hosp Infect 1991; 17:35–43
    [Google Scholar]
  9. Munoz J. L., Nebreda T., Garcia-Rodriguez J. A. [Development of a selective differential medium for the isolation of Corynebacterium urealyticum (group D-2).] Desarrollo de un medio selectivo diferencial para el aislamiento de Corynebacterium urealyticum (grupo D-2). Enferm Infecc Microbiol Clin 1994; 12:246–250
    [Google Scholar]
  10. Ryan M., Murray P. R. Prevalence of Corynebacterium urealyticum in urine specimens collected at a university-affiliated medical center. J Clin Microbiol 1994; 32:1395–1396
    [Google Scholar]
  11. Walkden D., Klugmann K. P., Vally S., Naidoo P. Urinary tract infection with Corynebacterium urealyticum in South Africa. Eur J Clin Microbiol Infect Dis 1993; 12:18–24
    [Google Scholar]
  12. Jacobs N. F., Perlino C. A. “Diphtheroid” pneumonia. South Med J 1979; 72:475–476
    [Google Scholar]
  13. Riegel P., Grimont P. A. D., De Briel D. Corynebacterium group D2 (“Corynebacterium urealyticum”) constitutes a new genomic species. Res Microbiol 1992; 143:307–313
    [Google Scholar]
  14. Garcia-Bravo M., Aguado J. M., Morales J. M., Noriega A. R. Influence of external factors in resistance of Corynebacterium urealyticum to antimicrobial agents. Antimicrob Agents Che mother 1996; 40:497–499
    [Google Scholar]
  15. Nieto E., Zapardiel J., Soriano F. Environmental contamination by Corynebacterium urealyticum in a teaching hospital. J Hosp Infect 1996; 32:78–79
    [Google Scholar]
  16. National Committee for Clinical Laboratory Standards Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved Standard M7-A3. Vilanova, PA: National Committee for Clinical Laboratory Standards; 1993
    [Google Scholar]
  17. Clarridge J. E., Spiegel C. A. Corynebacterium and miscellaneous irregular Gram-positive rods, Erysipelothrix, and Gardnerella. In Murray P. R., Baron E. J., Pfaller M. A., Tenover F. C., Yolken R. H. (eds) Manual of clinical microbiology 6th edn Washington, DC: ASM Press; 1995357–378
    [Google Scholar]
  18. Van Bosterhaut B., Claeys G., Gigi J., Wauters G. Isolation of Corynebacterium group D2 from clinical specimens. Eur J Clin Microbiol Infect Dis 1987; 6:418–419
    [Google Scholar]
  19. Larson E. L., McGinley K. J., Leyden J. J., Cooley M. E., Talbot G. H. Skin colonization with antibiotic-resistant (JK group) and antibiotic-sensitive lipophilic diphtheroids in hospitalized and normal adults. J Infect Dis 1986; 153:701–706
    [Google Scholar]
  20. Hollis D. G., Weaver R. E. Gram-positive organisms: a guide to identification. Atlanta, GA: Centers for Diseases Control; 1981
    [Google Scholar]
  21. Soriano F., Zapardiel J., Ponte C. Use of selective media for isolating Corynebacterium urealyticum from urine specimens. J Clin Microbiol 1994; 32:2630–2631
    [Google Scholar]
  22. Soriano F., Zapardiel J., Nieto E. Antimicrobial susceptibilities of Corynebacterium species and other non-spore-forming gram-positive bacilli to 18 antimicrobial agents. Antimicrob Agents Chemother 1995; 39:208–214
    [Google Scholar]
  23. Garcia-Rodriguez J. A., Garcia Sanchez J. E., Munoz-Bellido J. L., Nebreda Mayoral T., Garcia Sanchez E., Garcia I. In vitro activity of 79 antimicrobial agents against Corynebacterium group D2. Antimicrob Agents Chemother 1991; 35:2140–2143
    [Google Scholar]
  24. Soriano F., Ponte C., Santamaria M. Corynebacterium group D2 as a cause of alkaline-encrusted cystitis: report of four cases and characterization of the organisms. J Clin Microbiol 1985; 21:788–792
    [Google Scholar]
  25. Wichmann S., Wirsing von Koenig C. H., Becker-Boost E., Finger H. Isolation of Corynebacterium group JK from clinical specimens with a semiselective medium. J Clin Microbiol 1983; 19:204–206
    [Google Scholar]
  26. Soriano F., Rodriquez-Tudela J. L., Femandez-Roblas R., Aguado J. M., Santamaria M. Skin colonization by Corynebacterium groups D2 and JK in hospitalized patients. J Clin Microbiol 1988; 26:1878–1880
    [Google Scholar]
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