1887

Abstract

A method was developed to detect in urine by the polymerase chain reaction (PCR). A 457-bp fragment of the urease gene of was amplified by PCR. Before PCR, components disturbing the amplification had to be reduced. This was possible by diluting the urine 1 in 10 with distilled water and by the extraction of the DNA. Urine specimens from 41 patients with systemic lupus erythematosus (SLE) and 21 healthy individuals were treated by the dilution method and investigated by PCR for DNA. The results were compared with those obtained by culture and the detection rates of PCR and culture were found to be identical. Also there was no difference in the detection rates of from urine of SLE patients and healthy individuals; 10 (24.4%) of the 41 urine specimens from SLE patients and five (23.8%) of the 21 urine specimens from healthy individuals gave positive results for . The results of this study do not indicate a decisive role for in SLE.

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1997-05-01
2022-12-01
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References

  1. Shepard M. C., Lunceford C. D., Ford D. K. Ureaplasma urealyticum gen. nov., sp. nov.: proposed nomenclature for the human T (T-strain) mycoplasmas. Int J Syst Bacteriol 1974; 24:160–171
    [Google Scholar]
  2. Robertson J. A., Stemke G. W. Expanded serotyping scheme for Ureaplasma urealyticum strains isolated from humans. J Clin Microbiol 1982; 15:873–878
    [Google Scholar]
  3. Robertson J. A., Vekris A., Bebear C., Stemke G. W. Polymerase chain reaction using 16S rRNA gene sequences distinguishes the two biovars of Ureaplasma urealyticum. J Clin Microbiol 1993; 31:824–830
    [Google Scholar]
  4. McCormack W. M. Ureaplasma urealyticum: ecologic niche and epidemiologic considerations. Pediatr Infect Dis 1986; 5: Suppl 6S232–S233
    [Google Scholar]
  5. Harrison H. R. Cervical colonization with Ureaplasma urealyticum pregnancy outcome: prospective studies. Pediatr Infect Dis 1986; 5:266–269
    [Google Scholar]
  6. Horowitz S. Ureaplasma colonization of amniotic fluid in early mid-trimester and in premature rupture of membranes. IOM Lett 1992; 2:83
    [Google Scholar]
  7. Robertson J. A., Honore L. H., Stemke G. W., Jenkins H. J. The association of the parvo biotype of Ureaplasma urealyticum with cases of reproductive failure. IOM Lett 1992; 2:63
    [Google Scholar]
  8. Ginsburg K. S., Kundsin R. B., Walter C. W., Schur P. H. Ureaplasma urealyticum and Mycoplasma hominis in women with systemic lupus erythematosus. Arthritis Rheum 1992; 35:429–433
    [Google Scholar]
  9. Tan E. M., Cohen A. S., Fies J. F. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 1982; 25:1271–1277
    [Google Scholar]
  10. Robertson J. A. Bromothymol blue broth: improved medium for detection of Ureaplasma urealyticum (T-strain mycoplasma). J Clin Microbiol 1978; 7:127–132
    [Google Scholar]
  11. Shepard M. C., Lunceford C. D. Differential agar medium (A7) for identification of Ureaplasma urealyticum (human T mycoplasmas) in primary cultures of clinical material. J Clin Microbiol 1976; 3:613–625
    [Google Scholar]
  12. Albers A. C., Fletcher R. D. Simple method for quantitation of viable mycoplasmas. Appl Environ Microbiol 1982; 43:958–960
    [Google Scholar]
  13. Schleif R. F., Wensingk P. C. Practical methods in molecular biology. New York: Springer; 198198–99
    [Google Scholar]
  14. Blanchard A. Ureaplasma urealyticum urease genes; use of a UGA tryptophan codon. Mol Microbiol 1990; 4:669–676
    [Google Scholar]
  15. Blum H., Beier H., Gross H. J. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 1987; 8:93–99
    [Google Scholar]
  16. Taylor-Robinson D. The male reservoir of Ureaplasma urealyticum. Pediatr Infect Dis 1986; 5: Suppl 6S234–S235
    [Google Scholar]
  17. Cassell G. F. L., Waites K. B., Watson H. L., Crouse D. T., Harasawa R. R. Ureaplasma urealyticum intrauterine infection; role in prematurity and disease in newborns. Clin Microbiol Rev 1993; 6:69–87
    [Google Scholar]
  18. Taylor-Robinson D., Thomas B. J., Furr P. M., Keat A. C. The association of Mycoplasma hominis with arthritis. Sex Transm Dis 1983; 10: Suppl 4341–344
    [Google Scholar]
  19. Taylor-Robinson D., Furr P. M., Webster A. D. B. Ureaplasma urealyticum in immunocompromised host. Pediatr Infect Dis 1986; 5: Suppl 6S236–S238
    [Google Scholar]
  20. Lo S.-C., Tsai S., Benish J. R., Shih J. W.-K., Wear D. J., Wong D. M. Enhancement of HIV-1 cytocidal effects in CD4+ lymphocytes by the AIDS-associated mycoplasma. Science 1991; 251:1074–1076
    [Google Scholar]
  21. Davis J. W., Espinosa D., Williams K., Kissel V., Cabello F. C. Ureaplasma proteins react with a monoclonal antibody produced against an HLA-B27 antigen. IOM Lett 19901–283
    [Google Scholar]
  22. Kirchhoff H., Binder A., Runge N., Meier B., Jacobs R., Busche K. Pathogenic mechanisms in the Mycoplasma arthritidis polyarthritis of rats. Rheumatol Int 1989; 9:193–196
    [Google Scholar]
  23. Cole B. C., Atkin C. L. The Mycoplasma arthritidis T-cell mitogen, MAM: a model superantigen. Immunol Today 1991; 12:271–276
    [Google Scholar]
  24. Simecka J. W., Davis J. K., Davidson M. K., Ross S. E., Stadtlander C. T. K.-H., Cassell G. H. Mycoplasma diseases of animals. In Maniloff J., McElhaney R. N., Finch L. R., Baseman J. B. (eds) Mycoplasmas. Molecular biology and pathogenesis Washington, DC: American Society for Microbiology; 1992391–415
    [Google Scholar]
  25. Cole B. C., Griffiths M. M. Triggering and exacerbation of autoimmune arthritis by the Mycoplasma arthritidis superantigen MAM. Arthritis Rheum 1993; 36:994–1002
    [Google Scholar]
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